Quorum sensing regulates transcription of the pilin gene mshA1 of MSHA pilus in Vibrio parahaemolyticus

Gene ◽  
2021 ◽  
pp. 145961
Author(s):  
Junfang Sun ◽  
Xue Li ◽  
Yue Qiu ◽  
Xingfan Xue ◽  
Miaomiao Zhang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Vibrio Parahaemolyticus ◽  
Pilin Gene

scholarly journals Expression of the Type VI Secretion System 1 Component Hcp1 Is Indirectly Repressed by OpaR inVibrio parahaemolyticus

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Lizhi Ma ◽  
Yiquan Zhang ◽  
Xiaojuan Yan ◽  
Liping Guo ◽  
Li Wang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Stress Responses ◽  
Structural Component ◽  
Vibrio Parahaemolyticus ◽  
Secretion System ◽  
Upstream Region ◽  
Bacterial Protein ◽  
Type Vi Secretion System ◽  
Type Vi Secretion ◽  
System 1

The type VI secretion system (T6SS) is bacterial protein injection machinery with roles in virulence, symbiosis, interbacterial interaction, antipathogenesis, and environmental stress responses. There are two T6SS loci, T6SS1 and T6SS2, in the two chromosomes ofVibrio parahaemolyticus, respectively. This work disclosed that the master quorum sensing (QS) regulator OpaR repressed the transcription ofhcp1encoding the structural component Hcp1 of T6SS1 inV. parahaemolyticus, indicating that QS had a negative regulatory action on T6SS1. A singleσ54-dependent promoter was transcribed forhcp1inV. parahaemolyticus, and its activity was repressed by the OpaR regulator. Since the OpaR protein could not bind to the upstream region ofhcp1, OpaR would repress the transcription ofhcp1in an indirect manner.

scholarly journals Transcriptional Regulation of opaR, qrr2–4 and aphA by the Master Quorum-Sensing Regulator OpaR in Vibrio parahaemolyticus

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34622 ◽  
Author(s):  
Yiquan Zhang ◽  
Yefeng Qiu ◽  
Yafang Tan ◽  
Zhaobiao Guo ◽  
Ruifu Yang ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Quorum Sensing ◽  
Vibrio Parahaemolyticus

Quorum sensing modulates transcription of cpsQ-mfpABC and mfpABC in Vibrio parahaemolyticus

2013 ◽  
Vol 166 (3) ◽  
pp. 458-463 ◽  
Author(s):  
Dongsheng Zhou ◽  
Xiaojuan Yan ◽  
Fen Qu ◽  
Li Wang ◽  
Yiquan Zhang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Vibrio Parahaemolyticus

scholarly journals SKRINING DAN IDENTIFIKASI BAKTERI ANTI QUORUM SENSING ASAL TAMBAK UDANG VANAME PENGHAMBAT VIRULENSI Vibrio parahaemolyticus

2021 ◽  
Vol 16 (1) ◽  
pp. 61
Author(s):  
Emei Widiyastuti ◽  
Iman Rusmana ◽  
Munti Yuhana
Keyword(s):  
Quorum Sensing ◽  
16S Rrna ◽  
Bacillus Cereus ◽  
Vibrio Parahaemolyticus ◽  
Bacillus Amyloliquefaciens ◽  
Chromobacterium Violaceum ◽  
Pathogenicity Test ◽  
Bacillus Sp ◽  
Communication Signals

Vibriosis dapat dicegah dan dikendalikan dengan memanfaatkan mekanisme anti quorum sensing (AQS). Salah satu strategi anti quorum sensing dalam menghambat ekspresi faktor virulen dari Vibrio parahaemolyticus yaitu dengan mendegradasi sinyal komunikasi sel bakteri menggunakan AHL laktonase. Penelitian ini bertujuan untuk menyeleksi dan mengindentifikasi bakteri penghasil AHL laktonase yang berpotensi mampu menghambat virulensi bakteri patogen V. parahaemolyticus. Isolasi bakteri dilakukan dari sampel saluran pencernaan udang vaname, air, dan sedimen tambak. Sebanyak 18 dari 111 isolat yang diisolasi menunjukkan adanya aktivitas AQS terhadap bioindikator Chromobacterium violaceum. Hasil uji patogenitas secara in vitro pada agar darah didapatkan tiga isolat yang tidak menunjukkan aktivitas hemolisis yaitu B5, K4, dan S12. Hasil konfirmasi dan analisis gen aiiA menggunakan Blast-X menunjukkan bahwa isolat B5 dan S12 memiliki kesamaan dengan AHL laktonase pada Bacillus cereus, sedangkan K4 memiliki similaritas dengan AHL laktonase pada multispesies Bacillus sp. Hasil pensejajaran sekuen gen 16S rRNA ketiga isolat tersebut dengan data pada GenBank, teridentifikasi sebagai Bacillus siamensis (B5), Bacillus cereus (K4), dan Bacillus amyloliquefaciens (S12). Berdasarkan hasil uji antagonis dan uji kultur bersama disimpulkan bahwa isolat K4 bekerja dengan mekanisme AQS sedangkan isolat B5 dan S12 diduga berjalan dua mekanisme secara bersama yaitu antibiosis dan anti quorum sensing. Hasil penelitian ini menunjukkan bahwa ketiga isolat tersebut memiliki potensi sebagai kandidat agen biokontrol pada akuakultur sehingga perlu dilakukan uji lanjutan.Vibriosis can be prevented and controlled by utilizing the anti-quorum sensing (AQS) mechanism. One of the anti-quorum sensing mechanisms to inhibit the expression of virulent factors of Vibrio parahaemolyticus is by degrading the quorum sensing communication signals using AHL lactonase. The study aimed to select and identify AHL lactonase-producing bacteria that have the potentials to inhibit the virulence of V. parahaemolyticus. Several batches of bacteria were isolated from the digestive tract of vannamei shrimp, water, and sediment of shrimp ponds. There were 18 out of 111 isolates that showed AQS activity against Chromobacterium violaceum used as a bioindicator. In vitro pathogenicity test on blood agar showed that B5, K4, and S12 isolates showed gamma hemolysis activity. The results of confirmation and analysis of aiiA genes using Blast-X showed that B5 and S12 isolates have AHL lactonase similarities with Bacillus cereus, whereas K4 has similarities with multispecies Bacillus sp. Alignment results of the 16S rRNA gene sequences with GenBank data showed that B5, K4, and S12 isolates were identified as Bacillus siamensis, Bacillus cereus, and Bacillus amyloliquefaciens, respectively. The follow up antagonistic and coculture tests revealed that K4 uses the AQS mechanism, while B5 and S12 likely use antibiotic mechanism and anti quorum sensing to inhibit the virulent expression of V. parahaemolyticus. This study concludes that the three isolates have the potential to be used as biocontrol agents in brackishwater aquaculture. Further research is needed to determine the pathogenicity of AQS bacteria to vannamei shrimp and the effective concentration of AQS bacteria to inhibit the virulence of V. parahaemolyticus to vannamei shrimp by in vivo treatment.  

Quorum sensing regulates the transcription of lateral flagellar genes in Vibrio parahaemolyticus

2019 ◽  
Vol 14 (12) ◽  
pp. 1043-1053 ◽  
Author(s):  
Renfei Lu ◽  
Hao Tang ◽  
Yue Qiu ◽  
Wenhui Yang ◽  
Huiying Yang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Cell Density ◽  
Vibrio Parahaemolyticus ◽  
High Cell Density ◽  
High Cell ◽  
Regulatory Effect ◽  
Swarming Motility ◽  
Dnase I Footprinting ◽  
Regulation Mechanisms ◽  
Low Cell Density

Aim: Investigation of the lateral flagellar (Laf) genes transcription by the quorum sensing (QS) regulators AphA and OpaR in Vibrio parahaemolyticus. Materials & methods: Regulation mechanisms were assessed by combined utilization of swarming motility assay, qPCR, LacZ fusion, EMSA and DNase I footprinting. Results: AphA and OpaR oppositely regulate swarming motility and Laf genes. At high cell density, OpaR bound to the regulatory regions of motY-lafK-fliEFGHIJ, fliMNPQR-flhBA, fliDSTKLA-motAB and lafA to repress their transcription. At low cell density, AphA indirectly activated their transcription. Conclusion: OpaR repression of swarming motility was via its direct repression of Laf genes, while AphA exerted its regulatory effect on swarming motility through unknown regulator(s).

scholarly journals Quorum Sensing Regulators AphA and OpaR Control Expression of the Operon Responsible for Biosynthesis of the Compatible Solute Ectoine

2019 ◽  
Vol 85 (22) ◽  
Author(s):  
Gwendolyn J. Gregory ◽  
Daniel P. Morreale ◽  
Megan R. Carpenter ◽  
Sai S. Kalburge ◽  
E. Fidelma Boyd
Keyword(s):  
Quorum Sensing ◽  
Vibrio Parahaemolyticus ◽  
De Novo ◽  
Turgor Pressure ◽  
Compatible Solutes ◽  
Compatible Solute ◽  
Feed Forward ◽  
Content Type ◽  
Feed Forward Loop ◽  
Ectoine Biosynthesis

ABSTRACT To maintain the turgor pressure of the cell under high osmolarity, bacteria accumulate small organic compounds called compatible solutes, either through uptake or biosynthesis. Vibrio parahaemolyticus, a marine halophile and an important human and shellfish pathogen, has to adapt to abiotic stresses such as changing salinity. Vibrio parahaemolyticus contains multiple compatible solute biosynthesis and transporter systems, including the ectABC-asp_ect operon required for de novo ectoine biosynthesis. Ectoine biosynthesis genes are present in many halotolerant bacteria; however, little is known about the mechanism of regulation. We investigated the role of the quorum sensing master regulators OpaR and AphA in ect gene regulation. In an opaR deletion mutant, transcriptional reporter assays demonstrated that ect expression was induced. In an electrophoretic mobility shift assay, we showed that purified OpaR bound to the ect regulatory region indicating direct regulation by OpaR. In an aphA deletion mutant, expression of the ect genes was repressed, and purified AphA bound upstream of the ect genes. These data indicate that AphA is a direct positive regulator. CosR, a Mar-type regulator known to repress ect expression in V. cholerae, was found to repress ect expression in V. parahaemolyticus. In addition, we identified a feed-forward loop in which OpaR is a direct activator of cosR, while AphA is an indirect activator of cosR. Regulation of the ectoine biosynthesis pathway via this feed-forward loop allows for precise control of ectoine biosynthesis genes throughout the growth cycle to maximize fitness. IMPORTANCE Accumulation of compatible solutes within the cell allows bacteria to maintain intracellular turgor pressure and prevent water efflux. De novo ectoine production is widespread among bacteria, and the ect operon encoding the biosynthetic enzymes is induced by increased salinity. Here, we demonstrate that the quorum sensing regulators AphA and OpaR integrate with the osmotic stress response pathway to control transcription of ectoine biosynthesis genes in V. parahaemolyticus. We uncovered a feed-forward loop wherein quorum sensing regulators also control transcription of cosR, which encodes a negative regulator of the ect operon. Moreover, our data suggest that this mechanism may be widespread in Vibrio species.

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