scholarly journals Identification of biofilm-stage specific proteins associated with multidrug resistance and quorum sensing pathway in a pandemic strain of Vibrio parahaemolyticus isolated from India

2016 ◽  
Vol 45 ◽  
pp. 45-46 ◽  
Author(s):  
A. Dharmaprakash ◽  
S. Thomas
Keyword(s):  
Multidrug Resistance ◽  
Quorum Sensing ◽  
Vibrio Parahaemolyticus ◽  
Pandemic Strain ◽  
Specific Proteins

Elucidating the anti-biofilm and anti-quorum sensing potential of selenocystine against respiratory tract infections causing bacteria: in vitro and in silico studies

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Bharti Patel ◽  
Subrata Mishra ◽  
Indira K. Priyadarsini ◽  
Sirisha L. Vavilala
Keyword(s):  
Quorum Sensing ◽  
Respiratory Tract ◽  
Protein Function ◽  
Docking Studies ◽  
Klebsiella Pneumonia ◽  
Potential Candidate ◽  
In Silico Studies ◽  
Specific Proteins ◽  
Tract Infections

Abstract Bacteria are increasingly relying on biofilms to develop resistance to antibiotics thereby resulting in their failure in treating many infections. In spite of continuous research on many synthetic and natural compounds, ideal anti-biofilm molecule is still not found thereby warranting search for new class of molecules. The current study focuses on exploring anti-biofilm potential of selenocystine against respiratory tract infection (RTI)-causing bacteria. Anti-bacterial and anti-biofilm assays demonstrated that selenocystine inhibits the growth of bacteria in their planktonic state, and formation of biofilms while eradicating preformed-biofilm effectively. Selenocystine at a MIC50 as low as 42 and 28 μg/mL effectively inhibited the growth of Klebsiella pneumonia and Pseudomonas aeruginosa. The antibacterial effect is further reconfirmed by agar cup diffusion assay and growth-kill assay. Selenocystine showed 30–60% inhibition of biofilm formation in K. pneumonia, and 44–70% in P. aeruginosa respectively. It also distorted the preformed-biofilms by degrading the eDNA component of the Extracellular Polymeric Substance matrix. Molecular docking studies of selenocystine with quorum sensing specific proteins clearly showed that through the carboxylic acid moiety it interacts and inhibits the protein function, thereby confirming its anti-biofilm potential. With further validation selenocystine can be explored as a potential candidate for the treatment of RTIs.

Quorum sensing regulates transcription of the pilin gene mshA1 of MSHA pilus in Vibrio parahaemolyticus

Gene ◽  
2021 ◽  
pp. 145961
Author(s):  
Junfang Sun ◽  
Xue Li ◽  
Yue Qiu ◽  
Xingfan Xue ◽  
Miaomiao Zhang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Vibrio Parahaemolyticus ◽  
Pilin Gene

scholarly journals Expression of the Type VI Secretion System 1 Component Hcp1 Is Indirectly Repressed by OpaR inVibrio parahaemolyticus

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Lizhi Ma ◽  
Yiquan Zhang ◽  
Xiaojuan Yan ◽  
Liping Guo ◽  
Li Wang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Stress Responses ◽  
Structural Component ◽  
Vibrio Parahaemolyticus ◽  
Secretion System ◽  
Upstream Region ◽  
Bacterial Protein ◽  
Type Vi Secretion System ◽  
Type Vi Secretion ◽  
System 1

The type VI secretion system (T6SS) is bacterial protein injection machinery with roles in virulence, symbiosis, interbacterial interaction, antipathogenesis, and environmental stress responses. There are two T6SS loci, T6SS1 and T6SS2, in the two chromosomes ofVibrio parahaemolyticus, respectively. This work disclosed that the master quorum sensing (QS) regulator OpaR repressed the transcription ofhcp1encoding the structural component Hcp1 of T6SS1 inV. parahaemolyticus, indicating that QS had a negative regulatory action on T6SS1. A singleσ54-dependent promoter was transcribed forhcp1inV. parahaemolyticus, and its activity was repressed by the OpaR regulator. Since the OpaR protein could not bind to the upstream region ofhcp1, OpaR would repress the transcription ofhcp1in an indirect manner.

scholarly journals Enhancement of UV Light Sensitivity of a Vibrio parahaemolyticus O3:K6 Pandemic Strain Due to Natural Lysogenization by a Telomeric Phage

2009 ◽  
Vol 75 (6) ◽  
pp. 1697-1702 ◽  
Author(s):  
Beatriz Zabala ◽  
Katherine García ◽  
Romilio T. Espejo
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Uv Light ◽  
Bacterial Pathogen ◽  
Light Sensitivity ◽  
Alkaline Extraction ◽  
Clonal Population ◽  
Pandemic Strain ◽  
Clonal Strain ◽  
Similar Restriction

ABSTRACT The Vibrio parahaemolyticus O3:K6 pandemic clonal strain was first observed in southern Chile in 2004 and has since caused approximately 8,000 seafood-related diarrhea cases in this region. The massive proliferation of the original clonal population offers a unique opportunity to study the evolution of a bacterial pathogen in its natural environment by detection and characterization of emerging bacterial variants. Here, we describe a group of pandemic variants characterized by the presence of a 42-kb extrachromosomal DNA that can be recovered by alkaline extraction. Upon treatment with mitomycin C, these variants lyse with production of a myovirus containing DNA of equal size to the plasmid but which cannot be recovered by alkaline extraction. Plasmid and phage DNAs show similar restriction patterns corresponding to enzyme sites in a circular permutation. Sequenced regions showed 81 to 99% nucleotide similarity to bacteriophage VHML of Vibrio harveyi. Altogether these observations indicate that the 42-kb plasmid corresponds to a prophage, consisting of a linear DNA with terminal hairpins of a telomeric temperate phage with a linear genome. Bacteria containing the prophage were 7 to 15 times more sensitive to UV radiation, likely due to phage induction by UV irradiation as plasmid curing restored the original sensitivity. The enhanced UV sensitivity could have a significant role in reducing the survival and propagation capability of the V. parahaemolyticus pandemic strain in the ocean.

scholarly journals The first outbreak of acute diarrhea due to a pandemic strain of Vibrio parahaemolyticus O3:K6 in Kolkata, India

2007 ◽  
Vol 11 (2) ◽  
pp. 185-187 ◽  
Author(s):  
B. Sen ◽  
B. Dutta ◽  
S. Chatterjee ◽  
M.K. Bhattacharya ◽  
R.K. Nandy ◽  
...  
Keyword(s):  
Acute Diarrhea ◽  
Vibrio Parahaemolyticus ◽  
Pandemic Strain

Inhibitory activity of Thai condiments on pandemic strain of Vibrio parahaemolyticus

2007 ◽  
Vol 24 (4) ◽  
pp. 413-418 ◽  
Author(s):  
Varaporn Vuddhakul ◽  
Phuangthip Bhoopong ◽  
Fadeeya Hayeebilan ◽  
Sanan Subhadhirasakul
Keyword(s):  
Inhibitory Activity ◽  
Vibrio Parahaemolyticus ◽  
Pandemic Strain

scholarly journals LuxS-Mediated Quorum Sensing in Borrelia burgdorferi, the Lyme Disease Spirochete

2002 ◽  
Vol 70 (8) ◽  
pp. 4099-4105 ◽  
Author(s):  
Brian Stevenson ◽  
Kelly Babb
Keyword(s):  
Lyme Disease ◽  
Quorum Sensing ◽  
Borrelia Burgdorferi ◽  
Cell Communication ◽  
Factor H ◽  
Autoinducer 2 ◽  
Specific Proteins ◽  
Complement Regulator ◽  
Regulate Protein ◽  
Infection Processes

ABSTRACT The establishment of Borrelia burgdorferi infection involves numerous interactions between the bacteria and a variety of vertebrate host and arthropod vector tissues. This complex process requires regulated synthesis of many bacterial proteins. We now demonstrate that these spirochetes utilize a LuxS/autoinducer-2 (AI-2)-based quorum-sensing mechanism to regulate protein expression, the first system of cell-cell communication to be described in a spirochete. The luxS gene of B. burgdorferi was identified and demonstrated to encode a functional enzyme by complementation of an Escherichia coli luxS mutant. Cultured B. burgdorferi responded to AI-2 by altering the expression levels of a large number of proteins, including the complement regulator factor H-binding Erp proteins. Through this mechanism, a population of Lyme disease spirochetes may synchronize production of specific proteins needed for infection processes.

A new group of cosmopolitan bacteriophages induce a carrier state in the pandemic strain of Vibrio parahaemolyticus

2010 ◽  
Vol 12 (4) ◽  
pp. 990-1000 ◽  
Author(s):  
Roberto Bastías ◽  
Gastón Higuera ◽  
Walter Sierralta ◽  
Romilio T. Espejo
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Carrier State ◽  
Pandemic Strain
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