Long-term application of swine manure and sewage sludge differently impacts antibiotic resistance genes in soil and phyllosphere

Geoderma ◽  
2022 ◽  
Vol 411 ◽  
pp. 115698
Author(s):  
Xue-Mei Han ◽  
Hang-Wei Hu ◽  
Jin-Yang Li ◽  
He-Lian Li ◽  
Fang He ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Sewage Sludge ◽  
Resistance Genes ◽  
Swine Manure ◽  
Antibiotic Resistance Genes

scholarly journals Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil

2016 ◽  
Vol 92-93 ◽  
pp. 1-10 ◽  
Author(s):  
Qinglin Chen ◽  
Xinli An ◽  
Hu Li ◽  
Jianqiang Su ◽  
Yibing Ma ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Sewage Sludge ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Field Application ◽  
Term Field

Dispersion of Antibiotic Resistance Genes (ARGs) from stored swine manure biogas digestate to the atmosphere

2021 ◽  
Vol 761 ◽  
pp. 144108
Author(s):  
Yu Zhang ◽  
Yunhao Zheng ◽  
Zhiping Zhu ◽  
Yongxing Chen ◽  
Hongmin Dong
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Swine Manure ◽  
Antibiotic Resistance Genes ◽  
Biogas Digestate

Enrichment of antibiotic resistance genes in soil receiving composts derived from swine manure, yard wastes, or food wastes, and evidence for multiyear persistence of swine Clostridium spp.

2018 ◽  
Vol 64 (3) ◽  
pp. 201-208 ◽  
Author(s):  
Andrew Scott ◽  
Yuan-Ching Tien ◽  
Craig F. Drury ◽  
W. Daniel Reynolds ◽  
Edward Topp
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Swine Manure ◽  
Organic Amendments ◽  
Antibiotic Resistance Genes ◽  
Gene Target ◽  
Soil Dna ◽  
Yard Waste ◽  
The Impact ◽  
Clostridium Spp

The impact of amendment with swine manure compost (SMC), yard waste compost (YWC), or food waste compost (FWC) on the abundance of antibiotic resistance genes in soil was evaluated. Following a commercial-scale application of the composts in a field experiment, soils were sampled periodically for a decade, and archived air-dried. Soil DNA was extracted and gene targets quantified by qPCR. Compared with untreated control soil, all 3 amendment types increased the abundance of gene targets for up to 4 years postapplication. The abundance of several gene targets was much higher in soil amended with SMC than in soil receiving either YWC or FWC. The gene target ermB remained higher in the SMC treatment for a decade postapplication. Clostridia were significantly more abundant in the SMC-amended soil throughout the decade following application. Eight percent of Clostridium spp. isolates from the SMC treatment carried ermB. Overall, addition of organic amendments to soils has the potential to increase the abundance of antibiotic resistance genes. Amendments of fecal origin, such as SMC, will in addition entrain bacteria carrying antibiotic resistance genes. Environmentally recalcitrant clostridia, and the antibiotic resistance genes that they carry, will persist for many years under field conditions following the application of SMC.

Metagenomic analysis of effects of oxytetracycline and copper on antibiotic resistance genes and associated pathogenic hosts in swine manure compost

2020 ◽  
Author(s):  
Honghong Guo ◽  
jie gu ◽  
Xiaojuan Wang ◽  
Zilin Song ◽  
Xun Qian ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Swine Manure ◽  
Antibiotic Resistance Genes ◽  
Feed Additives ◽  
Poultry Feed ◽  
Risk Scores ◽  
Composting Process ◽  
Positive Effects

Abstract Background: The proliferation of antibiotic resistance genes (ARGs) in compost and their horizontal transfer to human pathogenic bacteria (HPB) may lead to the failure of human antibiotics. However, the antibiotic resistome in compost has not been comprehensively characterized. This study used a metagenomic approach to obtain new insights into the effects of oxytetracycline (OTC) and copper (Cu) on the antibiotic resistome during swine manure composting and the risks posed to human health. Results: The results showed that composting reduced the abundances and diversity of ARGs and HPB in swine manure. In total, 289 ARG subtypes and 19 ARG types were detected in the samples with abundances ranging from 1.08 ´ 10 –1 to 9.39 ´ 10 –1 copies/16S rRNA, which mainly encoded tetracycline, aminoglycoside, and macrolide–lincosamide–streptogramin (MLS) resistance genes. The application of OTC and Cu, especially the combined application, exacerbated the compost resistome risk scores and specific ARG subtypes responded differently. Tetracycline, multidrug, and MLS resistance genes mainly affected resistance profiles of HPB throughout the composting process. HPB and intI1 had significant positive effects on determining the ARG profiles during the composting process, and the co-selective effect of heavy metals may increase the abundances of ARGs via strong positive effects on intI1 . In addition, the effect of mobile genetic elements on the horizontal gene transfer of ARGs should not be ignored. Conclusions: This study of the antibiotic resistome in compost indicates the need for effective regulation of the misuse of livestock and poultry feed additives in order to minimize the spread of the antibiotic resistome in agro-ecosystems and decrease the potential risk to public health. Keywords: Antibiotic resistome; Composting; Metagenome; Pathogenic host; Swine manure

Microplastics act as vectors for antibiotic resistance genes in landfill leachate: The enhanced roles of the long-term aging process

2021 ◽  
Vol 270 ◽  
pp. 116278
Author(s):  
Yinglong Su ◽  
Zhongjian Zhang ◽  
Jundong Zhu ◽  
Jianhong Shi ◽  
Huawei Wei ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Landfill Leachate ◽  
Aging Process ◽  
Antibiotic Resistance Genes

scholarly journals Cohorting KPC+ Klebsiella pneumoniae (KPC-Kp)–positive patients: A genomic exposé of cross-colonization hazards in a long-term acute-care hospital (LTACH)

2020 ◽  
Vol 41 (10) ◽  
pp. 1162-1168
Author(s):  
Shawn E. Hawken ◽  
Mary K. Hayden ◽  
Karen Lolans ◽  
Rachel D. Yelin ◽  
Robert A. Weinstein ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Acute Care ◽  
Resistance Genes ◽  
Acute Care Hospital ◽  
Antibiotic Resistance Genes ◽  
Care Hospital ◽  
Cross Transmission ◽  
The Impact

AbstractObjective:Cohorting patients who are colonized or infected with multidrug-resistant organisms (MDROs) protects uncolonized patients from acquiring MDROs in healthcare settings. The potential for cross transmission within the cohort and the possibility of colonized patients acquiring secondary isolates with additional antibiotic resistance traits is often neglected. We searched for evidence of cross transmission of KPC+ Klebsiella pneumoniae (KPC-Kp) colonization among cohorted patients in a long-term acute-care hospital (LTACH), and we evaluated the impact of secondary acquisitions on resistance potential.Design:Genomic epidemiological investigation.Setting:A high-prevalence LTACH during a bundled intervention that included cohorting KPC-Kp–positive patients.Methods:Whole-genome sequencing (WGS) and location data were analyzed to identify potential cases of cross transmission between cohorted patients.Results:Secondary KPC-Kp isolates from 19 of 28 admission-positive patients were more closely related to another patient’s isolate than to their own admission isolate. Of these 19 cases, 14 showed strong genomic evidence for cross transmission (<10 single nucleotide variants or SNVs), and most of these patients occupied shared cohort floors (12 patients) or rooms (4 patients) at the same time. Of the 14 patients with strong genomic evidence of acquisition, 12 acquired antibiotic resistance genes not found in their primary isolates.Conclusions:Acquisition of secondary KPC-Kp isolates carrying distinct antibiotic resistance genes was detected in nearly half of cohorted patients. These results highlight the importance of healthcare provider adherence to infection prevention protocols within cohort locations, and they indicate the need for future studies to assess whether multiple-strain acquisition increases risk of adverse patient outcomes.

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