Microplastics act as vectors for antibiotic resistance genes in landfill leachate: The enhanced roles of the long-term aging process

AbstractObjective:Cohorting patients who are colonized or infected with multidrug-resistant organisms (MDROs) protects uncolonized patients from acquiring MDROs in healthcare settings. The potential for cross transmission within the cohort and the possibility of colonized patients acquiring secondary isolates with additional antibiotic resistance traits is often neglected. We searched for evidence of cross transmission of KPC+ Klebsiella pneumoniae (KPC-Kp) colonization among cohorted patients in a long-term acute-care hospital (LTACH), and we evaluated the impact of secondary acquisitions on resistance potential.Design:Genomic epidemiological investigation.Setting:A high-prevalence LTACH during a bundled intervention that included cohorting KPC-Kp–positive patients.Methods:Whole-genome sequencing (WGS) and location data were analyzed to identify potential cases of cross transmission between cohorted patients.Results:Secondary KPC-Kp isolates from 19 of 28 admission-positive patients were more closely related to another patient’s isolate than to their own admission isolate. Of these 19 cases, 14 showed strong genomic evidence for cross transmission (<10 single nucleotide variants or SNVs), and most of these patients occupied shared cohort floors (12 patients) or rooms (4 patients) at the same time. Of the 14 patients with strong genomic evidence of acquisition, 12 acquired antibiotic resistance genes not found in their primary isolates.Conclusions:Acquisition of secondary KPC-Kp isolates carrying distinct antibiotic resistance genes was detected in nearly half of cohorted patients. These results highlight the importance of healthcare provider adherence to infection prevention protocols within cohort locations, and they indicate the need for future studies to assess whether multiple-strain acquisition increases risk of adverse patient outcomes.

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Soil types influence the characteristic of antibiotic resistance genes in greenhouse soil with long-term manure application

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2020.122334 ◽

2020 ◽

Vol 392 ◽

pp. 122334 ◽

Cited By ~ 5

Author(s):

Lanjun Wang ◽

Jinhua Wang ◽

Jun Wang ◽

Lusheng Zhu ◽

Jeremy L. Conkle ◽

...

Keyword(s):

Antibiotic Resistance ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

Soil Types ◽

Greenhouse Soil ◽

Manure Application

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Prevalence of Antibiotic Resistance Genes and Bacterial Pathogens in Long-Term Manured Greenhouse Soils As Revealed by Metagenomic Survey

Environmental Science & Technology ◽

10.1021/es504157v ◽

2014 ◽

Vol 49 (2) ◽

pp. 1095-1104 ◽

Cited By ~ 142

Author(s):

Hua Fang ◽

Huifang Wang ◽

Lin Cai ◽

Yunlong Yu

Keyword(s):

Antibiotic Resistance ◽

Resistance Genes ◽

Bacterial Pathogens ◽

Antibiotic Resistance Genes ◽

Greenhouse Soils

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Evolution of Antibiotic Resistance and the Relationship between the Antibiotic Resistance Genes and Microbial Compositions under Long-Term Exposure to Tetracycline and Sulfamethoxazole

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16234681 ◽

2019 ◽

Vol 16 (23) ◽

pp. 4681 ◽

Cited By ~ 1

Author(s):

Bingbing Du ◽

Qingxiang Yang ◽

Ruifei Wang ◽

Ruimin Wang ◽

Qiang Wang ◽

...

Keyword(s):

Antibiotic Resistance ◽

Wastewater Treatment ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

The Core ◽

Aerobic Wastewater Treatment ◽

The Relationship ◽

Bacterial Groups ◽

Wastewater Treatment Systems

The removal of antibiotics and widespread of antibiotic resistance genes (ARGs) have received continuous attention due to the possible threats to environment. However, little information is available on the evolution of antibiotic resistance and the relationship between ARGs and microbial communities under long-term exposure to sub-inhibitory concentrations of antibiotics. In our study, two laboratory-scale anoxic-aerobic wastewater treatment systems were established and operated for 420 days to investigate the evolution of antibiotic resistance under exposure of 5 mg·L−1 tetracycline (TC) or 5 mg·L−1 TC and 1 mg·L−1 sulfamethoxazole (SMX). The average removal rates of TC and SMX were about 59% and 72%, respectively. The abundance of the main ARGs responsible for resistance to TC and SMX increased obviously after antibiotics addition, especially when TC and SMX in combination (increased 3.20-fold). The tetC and sul1 genes were the predominant genes in the development of TC and SMX resistance, in which gene sul1 had the highest abundance among all the detected ARGs. Network analysis revealed that under antibiotic pressure, the core bacterial groups carrying multiple ARGs formed and concentrated in about 20 genera such as Dechloromonas, Candidatus Accumulibacter, Aeromonas, Rubrivivax, in which intI1 played important roles in transferring various ARGs except sul3.

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Long-term application of swine manure and sewage sludge differently impacts antibiotic resistance genes in soil and phyllosphere

Geoderma ◽

10.1016/j.geoderma.2022.115698 ◽

2022 ◽

Vol 411 ◽

pp. 115698

Author(s):

Xue-Mei Han ◽

Hang-Wei Hu ◽

Jin-Yang Li ◽

He-Lian Li ◽

Fang He ◽

...

Keyword(s):

Antibiotic Resistance ◽

Sewage Sludge ◽

Resistance Genes ◽

Swine Manure ◽

Antibiotic Resistance Genes

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Community structure explains antibiotic resistance gene dynamics over a temperature gradient in soil

10.7287/peerj.preprints.3484 ◽

2017 ◽

Author(s):

Taylor K Dunivin ◽

Ashley Shade

Keyword(s):

Antibiotic Resistance ◽

Community Structure ◽

Temperature Gradient ◽

Resistance Genes ◽

Antibiotic Resistance Gene ◽

Antibiotic Resistance Genes ◽

Reference Surface ◽

Ambient Temperatures ◽

Concomitant Reduction

Soils are reservoirs of antibiotic resistance genes, but dynamics of antibiotic resistance genes in the environment are largely unknown. Long-term disturbances offer extended opportunities to examine microbiome responses at scales relevant for both ecological and evolutionary processes, and therefore can be insightful for studying the dynamics of antibiotic resistance genes in the environment. We examined antibiotic resistance genes in soils overlying the underground coal seam fire in Centralia, PA, which has been burning since 1962. As the fire progresses, previously hot soils can recover to ambient temperatures, which creates a gradient of contemporary and historical fire impact. We examined metagenomes from fire-affected, recovered, and reference surface soils to examine gene-resolved dynamics of antibiotic resistance using a gene-targeted assembler. We targeted 35 distinct types of clinically-relevant antibiotic resistance genes and two horizontal gene transfer-related genes (intI and repA). We detected 17 antibiotic resistance genes in Centralia, including AAC6-Ia, adeB, bla_A, bla_B, bla_C, cmlA, dfra12, intI, sul2, tetA, tetW, tetX, tolC, vanA, vanH, vanX, and vanZ. The diversity and abundance of several antibiotic resistance genes (bla_A, bla_B, dfra12, tolC) decreased with soil temperature, and changes in ARGs could largely be explained by associated changes in community structure. We also observed sequence-specific dynamics along the temperature gradient and observed compositional shifts in bla_A, dfra12, and intI. These results suggest that increased temperatures can reduce soil antibiotic resistance genes but that this is largely due to a concomitant reduction in community-level diversity.

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Community structure explains antibiotic resistance gene dynamics over a temperature gradient in soil

10.7287/peerj.preprints.3484v1 ◽

2017 ◽

Cited By ~ 1

Author(s):

Taylor K Dunivin ◽

Ashley Shade

Keyword(s):

Antibiotic Resistance ◽

Community Structure ◽

Temperature Gradient ◽

Resistance Genes ◽

Antibiotic Resistance Gene ◽

Antibiotic Resistance Genes ◽

Reference Surface ◽

Ambient Temperatures ◽

Concomitant Reduction

Soils are reservoirs of antibiotic resistance genes, but dynamics of antibiotic resistance genes in the environment are largely unknown. Long-term disturbances offer extended opportunities to examine microbiome responses at scales relevant for both ecological and evolutionary processes, and therefore can be insightful for studying the dynamics of antibiotic resistance genes in the environment. We examined antibiotic resistance genes in soils overlying the underground coal seam fire in Centralia, PA, which has been burning since 1962. As the fire progresses, previously hot soils can recover to ambient temperatures, which creates a gradient of contemporary and historical fire impact. We examined metagenomes from fire-affected, recovered, and reference surface soils to examine gene-resolved dynamics of antibiotic resistance using a gene-targeted assembler. We targeted 35 distinct types of clinically-relevant antibiotic resistance genes and two horizontal gene transfer-related genes (intI and repA). We detected 17 antibiotic resistance genes in Centralia, including AAC6-Ia, adeB, bla_A, bla_B, bla_C, cmlA, dfra12, intI, sul2, tetA, tetW, tetX, tolC, vanA, vanH, vanX, and vanZ. The diversity and abundance of several antibiotic resistance genes (bla_A, bla_B, dfra12, tolC) decreased with soil temperature, and changes in ARGs could largely be explained by associated changes in community structure. We also observed sequence-specific dynamics along the temperature gradient and observed compositional shifts in bla_A, dfra12, and intI. These results suggest that increased temperatures can reduce soil antibiotic resistance genes but that this is largely due to a concomitant reduction in community-level diversity.

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Cohorting KPC+ Klebsiella pneumoniae (KPC-Kp) positive patients – a genomic exposé of cross-colonization hazards in a long-term acute care hospital (LTACH)

10.1101/2020.02.07.20020669 ◽

2020 ◽

Author(s):

Shawn E. Hawken ◽

Mary K. Hayden ◽

Karen Lolans ◽

Rachel D. Yelin ◽

Robert A. Weinstein ◽

...

Keyword(s):

Antibiotic Resistance ◽

Klebsiella Pneumoniae ◽

Acute Care ◽

Resistance Genes ◽

Acute Care Hospital ◽

Antibiotic Resistance Genes ◽

Care Hospital ◽

Cross Transmission ◽

The Impact

AbstractObjectiveCohorting patients who are colonized or infected with multidrug-resistant organisms (MDROs) has been demonstrated to protect uncolonized patients from acquiring MDROs in healthcare settings. A neglected aspect of cohorting is the potential for cross-transmission within the cohort and the possibility of colonized patients acquiring secondary isolates with additional antibiotic resistance traits. We searched for evidence of cross-transmission of KPC+ Klebsiella pneumoniae (KPC-Kp) colonization among cohorted patients in a long-term acute care hospital (LTACH), and evaluated the impact of secondary acquisitions on resistance potential.DesignGenomic epidemiological investigationSettingA high-prevalence LTACH during a bundled intervention that included cohorting KPC-Kp-positive patients.MethodsWhole-genome sequencing (WGS) and location data were analyzed to identify potential cases of cross-transmission between cohorted patients.ResultsSecondary KPC-Kp isolates from 19 of 28 admission-positive patients were more closely related to another patient’s isolate than to their own admission isolate. In 14 of these 19 cases there was strong genomic evidence for cross-transmission (<10 SNVs) and the majority of these patients occupied shared cohort floors (12 cases) or rooms (5 cases) at the same time. Of the 14 patients with strong genomic evidence of acquisition, 12 acquired antibiotic resistance genes not found in their primary isolates.ConclusionsAcquisition of secondary KPC-Kp isolates carrying distinct antibiotic resistance genes was detected in nearly half of cohorted patients. These results highlight the importance of healthcare provider adherence to infection prevention protocols within cohort locations, and motivate future studies to assess whether multiple-strain acquisition increases risk of adverse patient outcomes.

Download Full-text