SCCmec typing of PVL-positive community-acquired Staphylococcus aureus (CA-MRSA) at a Japanese hospital

Abstract Objectives: Molecular typing such as spa typing is used to control and prevent of Staphylococcus. aureus widespread in hospitals and community acquired. Hence, the aim of this study was to find the common types of S. aureus strains circulating in Shiraz via spa and SCCmec typing methods. Results: Total of 159 S. aureus isolates were collected from two tertiary hospitals in Shiraz. Isolates were identified by biochemical tests. Antimicrobial susceptibility tests were performed by standard disk diffusion method and then genetic analysis of bacteria was performed using SCCmec and spa typing. In this study 31.4% of the isolates were methicillin-resistant S. aureus. The majority of isolates were SSC mec type ІІІ. Spa type t030 was the most prominent type among MRSA strains. In this study, for the first time in Iran, spa003, t386, t1877, t314, t186, t1816, t304, t325, t345 were reported. It was shown that there is a possibility that these spa types are natives to this region. Our findings showed that SCCmec ІІ, ІІІ and IV disseminate from hospital to community and vice versa. Thus, effective monitoring of MRSA in hospital and community is desirable. Keyword: Staphylococcus aureus , methicillin-resistant S. aureus (MRSA), SCCmec typing, spa typing

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SCCmec Typing and the Association of pvl, ACME, sea and seb Genes in Staphylococcus aureus Isolates From Burn Wound Infections

Avicenna Journal of Clinical Microbiology and Infection ◽

10.34172/ajcmi.2018.19 ◽

2018 ◽

Vol 5 (4) ◽

pp. 91-96

Author(s):

Hossein Motamedi ◽

Elahe Soltani Fard ◽

Mahshid Aria ◽

Seyyed Mojtaba Moosavian

Keyword(s):

Staphylococcus Aureus ◽

Wound Infections ◽

Burn Wound ◽

Sccmec Typing

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Characterization of SCCmec, spa types and Multi Drug Resistant of meticillin-resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran.

10.21203/rs.2.10702/v1 ◽

2019 ◽

Author(s):

zahra hashemizadeh ◽

nahal hadi ◽

samane mohebi ◽

davood kalantar-Neyestanaki ◽

abdolla bazargani

Keyword(s):

Staphylococcus Aureus ◽

Diffusion Method ◽

Biochemical Tests ◽

Spa Typing ◽

Disk Diffusion Method ◽

Methicillin Resistant ◽

Sccmec Typing ◽

Tertiary Hospitals ◽

Antimicrobial Susceptibility Tests ◽

Spa Types

Abstract Objectives: Molecular typing such as spa typing is used to control and prevent of Staphylococcus. aureus widespread in hospitals and community acquired. Hence, the aim of this study was to find the common types of S. aureus strains circulating in Shiraz via spa and SCCmec typing methods. Results: Total of 159 S. aureus isolates were collected from two tertiary hospitals in Shiraz. Isolates were identified by biochemical tests. Antimicrobial susceptibility tests were performed by standard disk diffusion method and then genetic analysis of bacteria was performed using SCCmec and spa typing. In this study 31.4% of the isolates were methicillin-resistant S. aureus. The majority of isolates were SSC mec type ІІІ. Spa type t030 was the most prominent type among MRSA strains. In this study, for the first time in Iran, spa003, t386, t1877, t314, t186, t1816, t304, t325, t345 were reported. It was shown that there is a possibility that these spa types are natives to this region. Our findings showed that SCCmec ІІ, ІІІ and IV disseminate from hospital to community and vice versa. Thus, effective monitoring of MRSA in hospital and community is desirable. Keyword: Staphylococcus aureus , methicillin-resistant S. aureus (MRSA), SCCmec typing, spa typing

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Staphylococcal cassette chroromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients attending Tengku Ampuan Afzan hospital (HTAA) in Kuantan, Pahang, Malaysia

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2012.05.594 ◽

2012 ◽

Vol 16 ◽

pp. e426 ◽

Cited By ~ 1

Author(s):

M.I. Mustafa ◽

N.A. Alarosi ◽

N. Amjad

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistant ◽

Sccmec Typing

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Methicillin-Resistant Staphylococcus aureus in Raw Milk: Prevalence, SCCmec Typing, Enterotoxin Characterization, and Antimicrobial Resistance Patterns

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-531 ◽

2015 ◽

Vol 78 (6) ◽

pp. 1142-1146 ◽

Cited By ~ 32

Author(s):

ALESSANDRA RIVA ◽

ELISA BORGHI ◽

DANIELA CIRASOLA ◽

SILVIA COLMEGNA ◽

FRANCESCA BORGO ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Dairy Products ◽

Raw Milk ◽

Antimicrobial Drugs ◽

Methicillin Resistant ◽

Sccmec Typing ◽

Multiplex Pcr Assay ◽

Genes Encoding ◽

Resistance Patterns ◽

Mrsa Strains

Staphylococcus aureus is a known major cause of foodborne illnesses, and raw milk and dairy products are often contaminated by enterotoxigenic and antimicrobial-resistant S. aureus strains. In the present study, 35 S. aureus strains were isolated from 383 raw milk samples collected from various dairy herds in the province of Milan (northern Italy). The isolates were characterized based on their antimicrobial susceptibility patterns and the presence of genes encoding staphylococcal enterotoxins (sea, seb, sec, sed, and see). About half (45.7%) of the strains were enterotoxigenic, and 37.1% were resistant to at least one of the antimicrobial drugs tested. Seven (20%) of 35 isolates were identified as methicillin-resistant S. aureus (MRSA), and SCCmec typing performed with a multiplex PCR assay revealed the presence of gene cassettes IV and V, typical of community-acquired MRSA, and I and II, characteristic of health care–associated MRSA. The MRSA strains were evaluated for the presence of the Panton-Valentine leukocidin gene, but this gene was not found. The results of the present study revealed the presence of toxin-producing S. aureus and MRSA strains in raw milk. MRSA and enterotoxigenic S. aureus in dairy farms are an important risk factor for the spread of staphylococcal infections; therefore, further studies are needed to find strategies for monitoring and controlling the presence of S. aureus, especially MRSA, in dairy products.

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SCCmec Genotypes of Methicillin-Resistant Staphylococcus aureus in Nasal Carriage of Multiple Sclerosis Patients in Iran

Iranian Journal of Public Health ◽

10.18502/ijph.v48i12.3560 ◽

2020 ◽

Author(s):

Yasaman JAMSHIDI ◽

Mohammad Reza POURMAND ◽

Zahra PAKBAZ ◽

Amirhossein POURMAND ◽

Abbas RAHIMI FOROUSHANI ◽

...

Keyword(s):

Multiple Sclerosis ◽

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Sccmec Type ◽

Nasal Carriage ◽

Control Group ◽

Sccmec Typing ◽

Multiple Sclerosis Patients ◽

And Control ◽

Sccmec Types

Background: Asymptomatic nasal colonization of Methicillin-Resistant Staphylococcus aureus is common in Multiple Sclerosis patients. SCCmec types I to III are mainly attributed to HA-MRSA strains whereas SCCmec types IV and V have commonly been reported in CA-MRSA infections. Here, we assessed the frequency of nasal carriage of MRSA in MS patients. This study aimed to evaluate MRSA SCCmec typing in MS nasal carriage. Methods: A cross-sectional descriptive study was conducted from Feb and Jun 2017 in MS Research Center, Tehran University of Medical Sciences (TUMS), Iran. Overall, 620 nasal swabs were collected (325 from MS patients and 295 from control group). Antimicrobial susceptibility test was performed using the disk diffusion and E-test method. Presence of mecA gene was confirmed by PCR assay and multiplex PCR was performed for SCCmec typing of MRSA isolates. Results: The frequency of MRSA among the MS patients and control group was almost equal (9.2% and 10.1%, respectively). SCCmec typing detected only types III, IV and V in both groups and type IV was the most predominant type in MS patients and control group. SCCmec type III was more prevalent in control group than MS patients (40% vs. 20%). Moreover, the frequency of SCCmec type V in MS patients was significantly higher than control group (36.7% vs. 3.3%). Conclusion: Although most MRSA isolates were collected from inpatients, interestingly there is a high frequency of SCCmec types IV and V in MS group. Moreover, MRSA isolates were not resistant to more antibiotics in SCCmec type III than types IV-V.

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SCCmec typing and Panton-valentine leukocidin occurrence in methicillin resistant Staphylococcus aureus (MRSA) isolates from clinical samples of Ahvaz,southwest of Iran

Microbiologia Medica ◽

10.4081/mm.2019.8050 ◽

2019 ◽

Vol 34 (2) ◽

Author(s):

Nikou Bahrami ◽

Hossein Motamedi ◽

Seyyedeh Elham Reza Tofighi ◽

Mohammad Reza Akhoond

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Multidrug Resistant ◽

Clinical Samples ◽

Methicillin Resistant ◽

Sccmec Typing ◽

Antibiotic Susceptibility Test ◽

High Prevalence ◽

Panton Valentine Leukocidin ◽

Valentine Leukocidin

Resistance to methicillin in methicillin resistant Staphylococcus aureus (MRSA) is dependent on mecA gene located on staphylococcal cassette chromosome (SCC). Both SCCmec type and Panton-Valentine leukocidin (PVL) affect S. aureus pathogenicity. Aim of this study was to investigate the prevalence of SCCmecA types and pvl genes among MRSA isolates from inpatients. During this cross-sectional study on 100 clinical isolates, following antibiotic susceptibility test, screening of mecA and pvl genes, as well as SCCmec typing, was done in a multiplex PCR technique. From the studied samples, 58 isolates were recognized as MRSA. The frequency of mecA and pvl was 58% and 4%, respectively. All of the MRSA were resistant to cefoxitin and had the highest sensitivity to chloramphenicol. The majority (77.5%) of MRSA was originated from wound samples. The SCCmec III was the most frequent type (22.4%) in these samples. The pvl positive isolates were from SCCmec IVb and V, thus meaning they are from CA-MRSA. These results show a high prevalence of MRSA in the studied region and a widespread prevalence of SCCmec I-V types. Furthermore, high prevalence of SCCmec III indicates the prevalence of multidrug resistant MRSA. This finding is a serious alarm for medical health care practitioners for the correct use of antibiotics in order to limit the spread of multidrug resistant strains. In addition, with regard to life threatening infections caused by pvl harbouring strains, early diagnosis and treatment of infections caused by these isolates should be mandatory.

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