scholarly journals Urinary Angiostatin: A Potential Biomarker for Diagnosis and Evaluation of Disease Severity in IgA Nephropathy

2015 ◽  
Vol 17 (2) ◽  
pp. S33
Author(s):  
R. Bu ◽  
G.Y. Cai ◽  
Q. Zhang ◽  
S. Liang ◽  
Y. Lu ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Disease Severity ◽  
Potential Biomarker

scholarly journals The levels of urinary joining chain containing IgG immune complexes are associated with disease severity in IgA nephropathy

2019 ◽  
Author(s):  
Guanhong Li ◽  
Cai Yue ◽  
Xiaohong Fan ◽  
Yubing Wen ◽  
Gang Chen ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Iga Nephropathy ◽  
Disease Severity ◽  
Immune Complexes ◽  
Kidney Diseases ◽  
Enzyme Linked Immunosorbent Assay ◽  
Creatinine Ratio ◽  
Operating Characteristics ◽  
Potential Biomarker ◽  
Tubulointerstitial Lesions

Abstract Background: Circulating levels of aberrantly glycosylated IgA1 and its immune complexes (IgA/IgG-IC) are elevated and correlated with disease severity in IgA nephropathy (IgAN). The pathologic IgA containing immune complexes deposits in the kidney were found to be in dimeric or polymeric forms, suggesting that those deposited IgA immune complexes contain joining chain, which is critical for the multimerization and transportation of IgA. Both the dimeric IgA and polymeric IgA can cause renal damage by inducing the release of cytokines from mesangial cells. We aimed to investigate the urinary J chain containing IgG immune complexes/creatinine ratio (UJGCR) and analyze its relationship with disease severity in IgAN. Methods: The UJGCR were measured by sandwich enzyme linked immunosorbent assay in 26 patients with IgAN, 31 patients with other kidney diseases and 32 healthy volunteers. Results: The levels of UJGCR were higher in patients with IgAN than that in non-IgAN patients ( P =0.006) and healthy volunteers ( P <0.0001). Importantly, receiver operating characteristics curve analysis confirmed that UJGCR had good discrimination between non-IgAN patients and IgAN patients. The levels of UJGCR positively correlated with 24-hour urinary protein excretion (r=0.63, P =0.0006), serum creatinine (r=0.55, P =0.003), and negatively correlated with estimated glomerular filtration rate (r= -0.61, P =0.0008) in IgAN. Furthermore, the levels of UJGCR were higher in IgAN patients with IgA mesangial deposition of (+/++) than patients with (+++/++++). And IgAN patients with tubular atrophy/interstitial fibrosis showed higher levels of UJGCR than that without ( P =0.03). Similarly, the levels of urinary IgA-IgG/creatinine ratio (UAGCR) were also found to be elevated and associated with clinical and pathological parameters as UJGCR in IgAN. Besides, significant correlation between the levels of UJGCR and UAGCR was shown, suggesting UJGCR was mainly composed of J-IgA-IgG. Conclusions: The levels of UJGCR are associated with disease severity in IgAN. UJGCR is a potential biomarker for both glomerular and tubulointerstitial lesions in IgAN.

scholarly journals The levels of urinary joining chain containing IgG immune complexes are associated with disease severity in IgA nephropathy

2019 ◽  
Author(s):  
Guanhong Li ◽  
Cai Yue ◽  
Xiaohong Fan ◽  
Yubing Wen ◽  
Gang Chen ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Iga Nephropathy ◽  
Disease Severity ◽  
Immune Complexes ◽  
Kidney Diseases ◽  
Enzyme Linked Immunosorbent Assay ◽  
Creatinine Ratio ◽  
Operating Characteristics ◽  
Potential Biomarker ◽  
Tubulointerstitial Lesions

Abstract Background: Circulating levels of aberrantly glycosylated IgA1 and its immune complexes (IgA/IgG-IC) are elevated and correlated with disease severity in IgA nephropathy (IgAN). The pathologic IgA containing immune complexes deposits in the kidney were found to be in dimeric or polymeric forms, suggesting that those deposited IgA immune complexes contain joining chain, which is critical for the multimerization and transportation of IgA. Dimeric IgA and polymeric IgA both can cause renal damage by inducing release of cytokines from mesangial cells. We aimed to investigate the urinary J chain containing IgG immune complexes/creatinine ratio (UJGCR) and analyze its relationship with disease severity in IgAN. Methods: The UJGCR were measured by sandwich enzyme linked immunosorbent assay in 26 patients with IgAN, 31 patients with other kidney diseases and 32 healthy volunteers. Results: The levels of UJGCR were higher in patients with IgAN than that in non-IgAN patients ( P =0.006) and healthy volunteers ( P <0.0001). Importantly, receiver operating characteristics curve analysis confirmed that UJGCR had good discrimination between non-IgAN patients and IgAN patients. The levels of UJGCR positively correlated with 24-hour urinary protein excretion (r=0.63, P =0.0006), serum creatinine (r=0.55, P =0.003), and negatively correlated with estimated glomerular filtration rate (r= -0.61, P =0.0008) in IgAN. Furthermore, the levels of UJGCR were higher in IgAN patients with IgA mesangial deposition of (+/++) than patients with (+++/++++). And IgAN patients with tubular atrophy/interstitial fibrosis showed higher levels of UJGCR than that without ( P =0.03). Similarly, the levels of urinary IgA-IgG/creatinine ratio (UAGCR) were also found to be elevated and associated with clinical and pathological parameters as UJGCR in IgAN. Besides, significant correlations between the levels of UJGCR and UAGCR were shown, suggesting UJGCR were mainly composed of J-IgA-IgG. Conclusions: The levels of UJGCR are associated with disease severity in IgAN. UJGCR is a potential biomarker for both glomerular and tubulointerstitial lesions in IgAN.

scholarly journals Elevated Endostatin Expression Is Regulated by the pIgA Immune Complex and Associated with Disease Severity of IgA Nephropathy

2020 ◽  
pp. 1-13
Author(s):  
Yaling Zhai ◽  
Xiaoqing Long ◽  
Jingge Gao ◽  
Xingchen Yao ◽  
Xinnian Wang ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Disease Severity ◽  
Immune Complex ◽  
Poor Prognosis ◽  
High Serum ◽  
Endothelial Cell Proliferation ◽  
Renal Interstitium ◽  
Before And After ◽  
Poor Outcomes

<b><i>Background/Aims:</i></b> Renal vascular injury accounts for the poor outcomes of patients with IgA nephropathy (IgAN). In this study, we investigated whether endostatin, a potent inhibitor of angiogenesis, is associated with IgAN. <b><i>Methods:</i></b> Serum endostatin levels were detected in patients with IgAN, disease controls, and healthy controls, and the correlation among endostatin and clinicopathologic manifestations, as well as prognosis in patients with IgAN, was analyzed. In addition, serum endostatin levels were compared in patients “before” and “after” treatment. Data on endostatin expression in the renal interstitium of patients with IgAN were downloaded and analyzed from the GSE35489 array in the GEO database. The poly-IgA1 (pIgA) immune complex is widely recognized as the “trigger” of IgAN initiation. pIgA in the plasma of patients was extracted and used to stimulate human glomerular endothelial cells (GECs). Endostatin, IL-6, and CXCL1 in the cell supernatant were detected by ELISA kits. <b><i>Results:</i></b> We found that serum endostatin levels were significantly increased in patients with IgAN, as was endostatin expression in the renal interstitium. Patients with IgAN were divided into 2 groups according to the median value. The high endostatin expression group had significantly higher levels of serum creatinine and BUN and more severe tubular/interstitial damage. Moreover, patients with arteriolar injury and endothelial cell proliferation had higher serum endostatin levels. Patients with high serum endostatin levels had poor prognosis. According to the in vitro experiment, the GEC apoptosis rate and the supernatant levels of endostatin, IL-6, and CXCL1 were significantly increased following pIgA stimulation. <b><i>Conclusion:</i></b> Our study found that elevated endostatin expression was associated with disease severity and poor prognosis in patients with IgAN and can be upregulated by pIgA, but how it participates in the pathogenesis of IgAN deserves further exploration.

scholarly journals Macular impairment in mitochondrial diseases: a potential biomarker of disease severity

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Guido Primiano ◽  
Edoardo Abed ◽  
Giovanni Corbo ◽  
Angelo Maria Minnella ◽  
Serenella Servidei ◽  
...  
Keyword(s):  
Disease Severity ◽  
Mitochondrial Diseases ◽  
Potential Biomarker

scholarly journals Correlational Analysis of ALS Progression and Serum NfL Measured by Simoa Assay in Chinese Patients

2020 ◽  
Vol 11 ◽  
Author(s):  
Kazuo Sugimoto ◽  
Yi Han ◽  
Yuebo Song ◽  
Ying Gao
Keyword(s):  
Disease Severity ◽  
Single Molecule ◽  
Clinical Relevance ◽  
Rating Scale ◽  
Diagnostic Delay ◽  
Chinese Patients ◽  
Progression Rate ◽  
Neurofilament Light Chain ◽  
Potential Biomarker ◽  
Als Patients

Background: Neurofilament light chain (NFL) was believed to be a promising biomarker for the diagnosis of Amyotrophic lateral sclerosis (ALS) and disease burden evaluation.Objective: To determine the serum NFL level and its clinical relevance, including its association with disease severity [evaluated by the ALS Functional Rating Scale–revised (ALSFRS-r) score and King's College staging system] and progression (evaluated by the disease progression rate (DPR) and diagnostic delay), in ALS patients in China.Method: Serum NFL levels were detected using the Single Molecule Array (Simoa) technology in 30 ALS patients and 20 healthy controls (HCs).Results: There were significantly elevated levels of serum NFL in patients with ALS than in the HCs (P &lt; 0.001). The serum NFL levels were significantly higher in rapidly progressive ALS and patients in Stage 3 than in slowly progressive ALS and patients in Stage 2 (PDPR &lt; 0.001, PDiagnosticdelay = 0.019; Pstage= 0.033). Furthermore, the serum NFL levels negatively correlated with the diagnostic delay (R2 = 0.23, P = 0.016), the ALSFRS-r score (R2 = 0.15, P = 0.047) and disease duration (R2 = 0.15, P = 0.034), and positively correlated with the DPR (R2 = 0.42, P &lt; 0.001).Conclusions: The present study preliminarily investigated the diagnostic value of serum NFL and its clinical relevance in the Chinese ALS population using the ultrasensitive Simoa technology. The results demonstrated that the level of serum NFL may become a potential biomarker for ALS diagnosis and indicate disease severity and progression.

scholarly journals Association of MEGSIN 2093C–2180T haplotype at the 3′ untranslated region with disease severity and progression of IgA nephropathy

2006 ◽  
Vol 21 (6) ◽  
pp. 1570-1574 ◽  
Author(s):  
Yunfeng Xia ◽  
Youji Li ◽  
Yong Du ◽  
Niansheng Yang ◽  
Caixia Li ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Disease Severity ◽  
Untranslated Region

scholarly journals Correlation of plasma and urine Wnt5A with the disease activity and cutaneous lesion severity in patients with systemic lupus erythematosus

2021 ◽  
Author(s):  
Shuhong Chi ◽  
Jing Xue ◽  
Xiaodong Chen ◽  
Xiaoming Liu ◽  
Yanhong Ji
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Disease Activity ◽  
Disease Severity ◽  
Lupus Erythematosus ◽  
Clinical Relevance ◽  
Function Analysis ◽  
Potential Candidate ◽  
Systemic Lupus ◽  
Cutaneous Involvement ◽  
Potential Biomarker

AbstractReliable noninvasive biomarkers are needed to accurately assess disease activity and prognosis in patients with systemic lupus erythematosus (SLE). The purpose of this study was to investigate the clinical relevance of Wnt5A with disease activity and severity with cutaneous involvement in particular in SLE patients; its concentrations in plasma and urine were examined and analyzed. In the cross-sectional study, the clinical relevance of Wnt5A protein was evaluated in both plasma and urine of SLE patients and healthy cohorts using commercial enzyme-linked immunosorbent assays (ELISA). Significantly, more abundances of Wnt5A protein were determined in both of plasmas and urines of SLE patients compared to healthy cohorts (p < 0.0001), which were even higher in active disease (AD) SLE patients relative to low disease activity (LDA) SLE patients (p < 0.0001). Meanwhile, the ROC curve analysis demonstrated that the plasma and urine Wnt5A were potential candidate biomarkers for identifying the disease activity and severity in SLE patients. The discriminant function analysis further revealed that the plasma and urine Wnt5A were separated and distinct for AD SLE patients and healthy controls. In consistence, the disease severity was correlated with the plasma and urine Wnt5A as ascertained by CLASI activity score and the prevalence of serositis in SLE patients. These results suggest that Wnt5A, as a summary measure for different inflammatory processes, could be a potential biomarker for accessing the disease activity, and a noninvasive biomarker for evaluating the disease severity in terms of cutaneous involvement in SLE patients.

scholarly journals High SARS-CoV-2 viral load is associated with a worse clinical outcome of COVID-19 disease

2021 ◽  
Vol 3 (9) ◽  
Author(s):  
María Eugenia Soria ◽  
Marta Cortón ◽  
Brenda Martínez-González ◽  
Rebeca Lobo-Vega ◽  
Lucía Vázquez-Sirvent ◽  
...  
Keyword(s):  
Viral Load ◽  
Disease Severity ◽  
Viral Evolution ◽  
High Viral Load ◽  
Viral Population ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Clinical Prognosis ◽  
Potential Biomarker ◽  
Potential Determinant

COVID-19 severity and progression are determined by several host and virological factors that may influence the final outcome of SARS-CoV-2-infected patients. The objective of this work was to determine a possible association between viral load, obtained from nasopharyngeal swabs, and the severity of the infection in a cohort of 448 SARS-CoV-2-infected patients from a hospital in Madrid during the first outbreak of the pandemic in Spain. To perform this, we clinically classified patients as mild, moderate and severe COVID-19 according to a number of clinical parameters such as hospitalization requirement, need of oxygen therapy, admission to intensive care units and/or death. Also, Ct values were determined using SARS-CoV-2-specific oligonucleotides directed to ORF1ab. Here we report a statistically significant association between viral load and disease severity, a high viral load being associated with worse clinical prognosis, independently of several previously identified risk factors such as age, sex, hypertension, cardiovascular disease, diabetes, obesity and lung disease (asthma and chronic obstructive pulmonary disease). The data presented here reinforce viral load as a potential biomarker for predicting disease severity in SARS-CoV-2-infected patients. It is also an important parameter in viral evolution since it relates to the numbers and types of variant genomes present in a viral population, a potential determinant of disease progression.

scholarly journals Assessment of cell free mitochondrial DNA as a biomarker of disease severity in different viral infections

2020 ◽  
Vol 36 (5) ◽  
Author(s):  
Zain Ali ◽  
Shahid Waseem ◽  
Riffat Aysha Anis ◽  
Mariam Anees
Keyword(s):  
Mitochondrial Dna ◽  
Viral Load ◽  
Disease Severity ◽  
Original Work ◽  
Viral Infections ◽  
Mt Dna ◽  
Creative Commons ◽  
Dna And Rna ◽  
Potential Biomarker ◽  
Open Access Article

Objective: Cell Free mitochondrial DNA (CF mt-DNA) has emerged as a novel biomarker to investigate disease pathophysiology of different infections. The present study was designed to elucidate the association between CF mt-DNA, IL-6 and viral load in HIV, HBV and HCV infections and predict its role as a potential biomarker to assess the disease severity in viral infections. Methods: Total 120 blood samples were collected from January 2018 to December 2018 of HIV, HBV and HCV patients and healthy controls (30 samples in each group). DNA and RNA were extracted from the serum to determine the levels of CF mt-DNA and viral load, respectively. IL-6 from the serum of infected individuals was quantified with ELISA. Results: HCV patients showed the highest levels of CF mt-DNA, IL-6 and viral load, followed by HBV and HIV. Significant correlation was found between CF mt-DNA and IL-6 among the HBV patients (p=0.017). However, no significant correlation of CF mt-DNA was observed with IL-6 in HIV and HCV or with the viral load in any of the three infections. Conclusion: Elevated CF mt-DNA indicates its role in severity of viral infections. Independence of CF mt-DNA expression from viral load and IL-6 in case of HIV and HCV suggests involvement of other inflammatory pathways regulating CF mt-DNA elevation. doi: https://doi.org/10.12669/pjms.36.5.2476 How to cite this:Ali Z, Waseem S, Anis RA, Anees M. Assessment of cell free mitochondrial DNA as a biomarker of disease severity in different viral infections. Pak J Med Sci. 2020;36(5):---------. doi: https://doi.org/10.12669/pjms.36.5.2476 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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