Influence of pretreatments on microtensile bond strength to eroded dentin using a universal adhesive in self-etch mode

SUMMARY Objectives: The aim of this study was to investigate the effect of different protocols of 17% ethylene diamine tetra-acetic acid (EDTA) conditioning on the etching pattern and immediate bond strength of universal adhesives to enamel and sclerotic dentin. Methods and Materials: Forty bovine teeth with sclerotic dentin and 20 human third molars were randomly divided into eight groups resulting from the combination of the main factors surface treatment (none, two-minute EDTA conditioning manual application, 30-second EDTA manual application, 30-second EDTA sonic application) and adhesives systems (Scotchbond Universal Adhesive [SBU] and Prime & Bond Elect [PBE]). Resin-dentin and enamel-dentin bond specimens were prepared and tested under the microtensile bond strength (μTBS) and microshear bond strength (μSBS) tests, respectively. The etching pattern produced on the unground enamel and the sclerotic dentin surfaces under the different protocols and adhesive systems was evaluated under scanning electron microscopy. Results: For enamel, only the main factor adhesive was significant (p<0.0001), with SBU showing the highest μSBS. In sclerotic dentin, the lowest mean was observed for the group without EDTA application and the highest mean in the group with EDTA application with the sonic device for 30 seconds. Regardless of the EDTA protocol, the highest means of μTBS were observed for SBU (p<0.05). Conclusions: EDTA conditioning improves the bonding performance of universal adhesives in the self-etch mode on sclerotic dentin, mainly when applied for 30 seconds with the aid of a sonic device. EDTA pretreatment also improves the retentive etching pattern of enamel, but it does not result in higher enamel bond strength.

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Effect of Different Application Techniques of Universal Bonding System on Microtensile Bond Strength of Bulk-Fill Composites to Primary and Permanent Dentin

Frontiers in Dentistry ◽

10.18502/fid.v17i1.3962 ◽

2020 ◽

Author(s):

Masoud Fallahinejad Ghajari ◽

Mahsa Sheikholeslamian ◽

Amir Ghasemi ◽

Leila Simaei

Keyword(s):

Bond Strength ◽

Failure Modes ◽

In Vitro Study ◽

Permanent Teeth ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Bonding System ◽

The Mean ◽

Self Etch

Objectives: This study aimed to determine the microtensile bond strength (μTBS) of a bulk-fill composite to permanent and primary coronal dentin using a universal adhesive in self-etch and total-etch modes. Materials and Methods: This in-vitro study was performed on 52 occlusal dentinal surfaces of human primary and permanent teeth. The crowns were cut to the gingival level. The 48 prepared dentin sections were randomly assigned to the following groups (n=13): A: Primary/Total-etch, B: Primary/Self-etch, C: Permanent/Total-etch, and D: Permanent/Self-etch. In groups A and C, after etching for 15 seconds, two layers of a universal bonding (Futurabond U) were applied and cured for 10 seconds. All samples were filled with a bulk-fill composite (x-trafil; VOCO) and cured for 40 seconds. The samples were cut to a bar-shaped dentin block with the dimensions of 1×1×1 mm3, and after 10,000 thermocycles, the μTBS test was accomplished at a crosshead speed of 1 mm/minute. The mean and standard deviation (SD) of μTBS were calculated, and the data were analyzed using two-way analysis of variance (ANOVA) and Fisher's exact test. Results: The mean μTBS was as follows: A: 15.03±2.0279, B: 11.11±2.4423, C: 23.50±4.8165, and D: 16.26±6.3200 MPa. Futurabond U showed a higher μTBS in the total-etch mode (P<0.001). The permanent teeth had greater μTBS than the primary teeth (P<0.001). Similar percentages of failure modes were observed in the total-etch groups but in the self-etch groups, most failures were in the form of adhesive and mixed. Conclusion: Greater μTBS was observed in the permanent teeth with the total-etch technique.

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Does Bonding Approach Influence the Bond Strength of Universal Adhesive to Dentin of Primary Teeth?

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4628-41.3.214 ◽

2017 ◽

Vol 41 (3) ◽

pp. 214-218 ◽

Cited By ~ 1

Author(s):

Tathiane Larissa Lenzi ◽

Fabio Zovico Maxnuck Soares ◽

Rachel de Oliveira Rocha

Keyword(s):

Bond Strength ◽

Adhesive System ◽

Primary Tooth ◽

Adhesive Systems ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Etch And Rinse ◽

Tooth Dentin ◽

Self Etch ◽

One Way Anova

Objective: To evaluate the effect of bonding strategy on microtensile bond strength (μTBS) of a new universal adhesive system to primary tooth dentin. Study design: Flat dentin surfaces from 25 primary molars were assigned to 5 groups according to the adhesive and bonding approach: Adper Single Bond 2 (two-step etch-and-rinse adhesive) and Clearfil SE Bond (two-step self-etch system), as controls; Scotchbond Universal Adhesive–self-etch, dry or wet-bonding etch-and-rinse strategies. Composite buildups were constructed and the teeth were sectioned to obtain bonded sticks (0.8 mm2) to be tested under tension at 1mm/min. The μTBS means were analyzed by one-way ANOVA and Tukey's tests (α = 0.05). Failure mode was evaluated using a stereomicroscope (400×). Results: Universal adhesive applied following both dry and wet-bonding etch-and-rinse strategies showed similar bond strength compared with control adhesive systems. Self-etch approach resulted in the lowest μTBS values. For all groups, adhesive/mixed failure prevailed. The percentage of premature debonded specimens was higher when the universal adhesive was used as self-etch mode. Conclusion: The universal adhesive does not share the same versatility of being used in the etch-and-rinse and self-etch approaches; however, the use of the new adhesive following either wet or dry-bonding may be a suitable option as alternative to two-step etch-and-rinse adhesive protocol.

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Microtensile bond strength of a universal adhesive to deep dentin

Brazilian Dental Science ◽

10.14295/bds.2016.v19i2.1259 ◽

2016 ◽

Vol 19 (2) ◽

pp. 104

Author(s):

Cristina Gre ◽

MAuro Amaral Caldeira de Andrada ◽

Sylvio Monteiro Junior

Keyword(s):

Bond Strength ◽

Water Storage ◽

Adhesive System ◽

Single Bond ◽

Third Molars ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

The Mean ◽

Self Etch ◽

One Way Anova

Objective: This study evaluated the microtensile bond strength of a universal adhesive system applied to deep dentin under different bonding strategies. Material and Methods: Fifteen human third molars had the coronal portion removed exposing deep dentin and were assigned into 3 groups according to the adhesive system: G1(control): 2-steps totaletch (Adper Single Bond 2, 3M ESPE); G2: 1-step self-etch universal adhesive (Scotchbond Universal, 3M ESPE); G3:2 steps total-etch universal adhesive (Scotchbond Universal, 3M ESPE). Composite buildups were performed on the dentin surfaces and after water storage for 24 h, teeth were sectioned to obtain 40 bonded beams per group with sectional area of 0.9 mm2 . The specimens were submitted to the microtensile bond strength (µTBS) test until failure. Statistical analyses were computed using one-way ANOVA (p = 0.05). Results: The mean µTBS (in MPa) were G1 = 22.27, G2 = 22.85 and G3 = 20.3. After statistical analysis, no significant differences were observed among the groups. Conclusions: Universal adhesive system performed similarly to the total-etch adhesive and was not affected by the adhesion strategy. KEYWORDS Microtensile; Adhesive system; Dentin.

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Evaluation of Microtensile Bond Strength of Universal Self-etch Adhesive System to Wet and Dry Dentin

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.4109 ◽

2020 ◽

Vol 8 (D) ◽

pp. 77-81

Author(s):

Mohamed Ahmed Wakwak ◽

Eslam Hassan Gabr ◽

Ahmed Mohamed Elmarakby

Keyword(s):

Bond Strength ◽

Adhesive System ◽

Solvent Free ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Significance Level ◽

Significant Difference ◽

One Step ◽

Storage Times ◽

Self Etch

BACKGROUND: The durability of dentin-resin interfaces with the universal adhesive system is a crucial characteristic with chemical interactions between the exposed collagen and the adhesive monomers, but it is still compromised with wet and dry mode. AIM: The present study evaluated the effect of dentin wetness and solvents containing of one-step self-etch adhesives on the microtensile bond strength (μTBS) of dentin at different storage times. METHODS: Occlusal dentin of 54 extracted human molars was exposed. Each adhesive agent was applied according to manufacturer instructions to wet and dry dentin surfaces. Composite resin was incrementally built up. Bond strengths to dentin were determined using the μTBS test after water storage for 24 h, 1 month, and 6 months. One-way ANOVA was used to compare between more than two non-related samples. The significance level was set at p ≤ 0.05. RESULTS: Dryness of dentin increases the μTBS with solvent-containing adhesives while decrease the μTBS with solvent-free adhesive. There was an increase in microtensile bond strength values in the case of ethanol water-based self-etch adhesive over time. No statistically significant difference was found among different storage times regarding μTBS for solvent-free adhesive, while a statistically significant difference was found among different storage times in μTBS for solvent-containing adhesives. CONCLUSION: Universal adhesive systems improve the durability and stability of dentin bond strength.

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Microtensile Bond Strength of Composite to Enamel Using Universal Adhesive with/without Acid Etching Compared To Etch and Rinse and Self-Etch Bonding Agents

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2018.427 ◽

2018 ◽

Vol 6 (11) ◽

pp. 2186-2192 ◽

Cited By ~ 4

Author(s):

Hoda Pouyanfar ◽

Elaheh Seyed Tabaii ◽

Samaneh Aghazadeh ◽

Seyyed Pedram Tabatabaei Navaei Nobari ◽

Mohammad Moslem Imani

Keyword(s):

Phosphoric Acid ◽

Bond Strength ◽

Testing Machine ◽

Acid Etching ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Bonding Agents ◽

Modes Of Failure ◽

Etch And Rinse ◽

Self Etch

AIM: Considering the recent introduction of universal adhesives and the controversy regarding the use/no use of etchant prior to their application, this study sought to assess the microtensile bond strength of composite to enamel using universal adhesive with/without acid etching compared to three-step etch and rinse, two-step etch and rinse and two-step self-etch bonding agents. METHODS: This in vitro, an experimental study was conducted on 80 extracted sound human molars in five groups (16 each): Scotchbond Universal adhesive (3M) with/without prior etching, Adper Scthe otchbond Multi-Purpose, Single Bond and Clearfil SE Bond. Etching was performed with 37% phosphoric acid for 20 seconds followed by rinsing and drying. The bonding agent was then applied and light-cured. The e-lite composite was bonded to surfaces and light-cured. The teeth were then mounted, sectioned and subjected to microtensile bond strength test in a universal testing machine. The mode of failure was, determined under a stereomicroscope. Data were analysed using one-way ANOVA followed by Tukey’s test. RESULTS: Universal adhesive with prior etching yielded the highest bond strength (P = 0.03). Pairwise comparisons showed that the bond strength of this group was significantly higher than that of universal adhesive without prior etching (P = 0.04). No other significant differences were noted (P > 0.05). The modes of failure were significantly different among the groups (P = 0.003). CONCLUSION: Enamel etching with phosphoric acid can significantly increase the bond strength to universal adhesive. Universal adhesive without prior etching provided the bond strength as high as that provided by etching and rinse and self-etch bonding agents.

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Modified Microtensile Bond Strength of Glass Ionomer to Composite Resin Using Universal Adhesive in Self-etch and Total-etch Modes

The Open Dentistry Journal ◽

10.2174/1874210602014010390 ◽

2020 ◽

Vol 14 (1) ◽

pp. 390-395

Author(s):

Hamid Kermanshah ◽

Ladan R. Omrani ◽

Omid Hemati ◽

Prham Pedram ◽

Elham Ahmadi

Keyword(s):

Bond Strength ◽

Composite Resin ◽

Testing Machine ◽

Glass Ionomer ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Bonding Agents ◽

Failure Data ◽

Self Etch ◽

One Way Anova

Background: Glass Ionomer Cements (GICs) are frequently used as base or liner before the application of restorative materials. The success of this approach depends on the bond strength of GICs to composite resin. Objectives: This study to assess the modified microtensile bond strength of glass ionomer to composite resin using universal adhesive in self-etch and total-etch modes. Methods: Samples were fabricated of resin-modified GIC (RMGIC) and conventional GIC(CGIC) (6 x 1 x 1 mm), and were randomly divided into 8 groups. Clearfil SE Bond and G-Premio universal adhesive in self-etch and total-etch modes were used according to the manufacturers’ instructions. Z250 composite was applied over the GIC (12 x 1 x 1 mm), and light-cured. The microtensile bond strength was measured using a universal testing machine. The samples in each group were evaluated under an electron microscope to determine the mode of failure. Data were analyzed using one-way ANOVA and Tukey’s test. Results: The microtensile bond strength of RMGI used with Clearfil SE Bond was significantly higher than that of other groups (6.57±1.15 MPa) (P<0.05). The maximum and minimum microtensile bond strength values of CGIC after applying the bonding agents were recorded after using G-Premio total-etch mode (1.34±0.77 MPa) and SE Bond in total-etch mode(1.18±79 MPa), respectively. Conclusion: Application of of G-Premio in both modes did not show any significant different bond strength in both glass ionomers. The bond strength of RMGIC was higher than that of CGIC, and the maximum bond strength of RMGI was achieved by the use of SE Bond.

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Impact of Immediate Dentin Sealing Using Universal Adhesive under Simulated Pulp Pressure on Microtensile Bond Strength of Indirect Resin Composite Restorations and Dentin Permeability

European Journal of Dentistry ◽

10.1055/s-0041-1735442 ◽

2021 ◽

Author(s):

Emad Abd Elfatah Abo-Alazm ◽

Rehab Khalil Safy

Keyword(s):

Bond Strength ◽

Resin Composite ◽

Water Storage ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Dentin Permeability ◽

Composite Restorations ◽

Significant Difference ◽

Cad Cam ◽

Self Etch

Abstract Objective The aim of this study was to investigate the effect of immediate dentin sealing (IDS) technique using universal adhesive under simulated pulp pressure on microtensile bond strength (µTBS) of indirect resin composite restorations and dentin permeability. Materials and Methods Fifty extracted caries-free human third molars were used for specimens' preparation. Each molar's occlusal table was abraded flat and their roots were separated under continuous water cooling. Forty specimens were used for microtensile bond strength test (µTBST) evaluation. The µTBST specimens were randomly assigned to two groups according to the dentin sealing time; Immediate dentin sealing (IDS) and delayed dentin sealing (DDS). Each group was further subdivided into two subgroups according to the adhesive system used for dentin sealing: iBOND self-etch adhesive and GLUMA Bond Universal. All specimens were exposed to simulated pulp pressure for 1 week then restored using computer-aided design/computer-aided manufacturing (CAD/CAM) resin composite blocks. The µTBS was evaluated for all tested subgroups after 24 hours and 6 months of water storage. The remaining 10 teeth were used for the preparation of dentin discs for dentin permeability evaluation. They were divided into two groups according to type of self-etch adhesive used. Fluid filtration rate was evaluated after etching, with smear layer and after adhesive application. Results obtained were statistically analyzed using Shapiro–Wilk test and Weibull analysis. Results Statistically significant difference was recorded between μTBS mean values of both IDS and DDS techniques at 24 hours and after 6 months of water storage. GLUMA Bond Universal adhesive had significantly higher bond strength compared with iBond at both IDS and DDS techniques, but both adhesives showed a significant reduction in the Weibull characteristic strength after 6 months of water storage. Significant reduction in dentin permeability was recorded by both adhesives without any significant difference between them. Conclusions The IDS technique using universal adhesive in self-etch mode is an effective strategy for improving the final bond strength of CAD/CAM resin composite restorations and reducing dentin permeability.

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Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

Marine Drugs ◽

10.3390/md18050263 ◽

2020 ◽

Vol 18 (5) ◽

pp. 263 ◽

Cited By ~ 1

Author(s):

Eugenia Baena ◽

Sandra R Cunha ◽

Tatjana Maravić ◽

Allegra Comba ◽

Federica Paganelli ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Bond Strength ◽

Adhesive Systems ◽

Microtensile Bond Strength ◽

Bond Stability ◽

Etch And Rinse ◽

Mechanical Durability ◽

Human Dentine ◽

Self Etch ◽

Metalloproteinase Activity

The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 s) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin–dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.

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