Autologous Tooth Dentin Graft: A Retrospective Study in Humans

Background and Objectives: The aim of this study is to evaluate the efficacy of an autologous dentin graft, via extracted teeth that are processed into bacteria-free particulate dentin in a Smart dentin grinder and then grafted immediately into alveolus post extraction or into bone deficiencies. Materials and Methods: Ten healthy, partially edentulous patients with some teeth in the mandible were recruited in the study. After their own teeth were grinded, particulate teeth were placed in empty sockets and bone defects after teeth extractions. Furthermore, after three, six, 12 and 24 months, core samples using a 3 mm trephine were obtained. Results: At three months, the particles of grinded tooth were immersed inside a new connective tissue with a small new bone formation (16.3 ± 1.98). At six months, we observed small particles of dentin integrated in new immature bone, without inflammation in the soft tissue (41.1 ± 0.76). At twelve months, we observed a high amount of bone formation surrounding tooth particles (54.5 ± 0.24), and at twenty-four months, new bone, a big structure of bone, was observed with dentin particles (59.4 ± 1.23), statistically different when compared it with at three months. Conclusions: A particulate dentin graft should be considered as an alternative material for sockets’ preservation, split technique, and also for sinus lifting. One of the special characteristics after 24 months of evaluation was the high resorption rate and bone replacement without inflammation. This material could be considered as an acceptable biomaterial for different bone defects due to its osteoinductive and osteoconductive properties

Clinical and laboratory studies of the effectiveness of a non-invasive method of treating caries in the stage of white chalky spot with Icon technology

The aim of the study was to evaluate the effectiveness of a non-invasive method of treating caries at the stage of a white chalky spot with the Infiltration Concept (Icon) technology. Materials and methods: the clinical and laboratory study included 5 teeth with caries in the white spot stage, removed according to orthodontic indications. The study included scanning in an X-ray computer microtomograph of selected samples of teeth with caries in the white spot stage before and after treatment of the caries area according to Icon technology. For each sample, 1601 X-ray projections were obtained, which were then reconstructed using the XRM Reconstructor software. The comparison of the mineralization density of the samples was carried out by calibrating the obtained images, reconstructing three-dimensional models of tooth samples and constructing maps of the mineralization fee. Results and conclusions. Data on the mineralization density before and after treatment according to Icon technology were obtained in the following areas of the studied teeth: caries in the white spot stage; dentin bordering on caries (the area touches the dentinoemal border as close as possible to the caries area); the area of healthy enamel on the opposite side of the tooth; dentin bordering on healthy enamel (near the dentinoemal border and as close as possible to the healthy enamel area). A comparison of the results obtained does not allow us to conclude about the effectiveness of treatment according to the Icon technology. Moreover, the mineralization density of the studied areas significantly decreased after the treatment.

Effect of Root Canal Filling Materials and Pretreatment with Solvents on the Shear Bond Strength of Composite Resin with Primary Tooth Dentin

Aim. Root canal filling materials have the tendency to inhibit adhesion of resin-based composites. This study was aimed at evaluating the effect of root canal filling materials and their solvents on the shear bond strength (SBS) of resin composite with the primary tooth dentin. Methods and Materials. Seventy-two intact anterior primary teeth were selected. Smooth dentinal surfaces were prepared to a minimum diameter of 3 mm and thickness of 1.5–2.0 mm. The samples were equally divided into six groups ( n = 12 ). In group 1: control group, no root filling material; in group 2: Metapex, no solvent; in group 3: Metapex+ethanol solvent; in group 4: ZOE, no solvent; in group 5: ZOE+ethanol solvent; and in group 6: ZOE+orange oil solvent were applied. Then, dentin surfaces were etched, and composite restorations were placed and cured. The specimens were stored in distilled water at 37°C for 24 hours. SBS values were determined using a universal testing machine. Results. The SBS values of composite to dentin in groups 2 and 4 were significantly lower than those in the control group ( P < 0.001 ). Cleansing of the specimens with 96% ethanol after removal of Metapex significantly increased the composite-dentin bond ( P < 0.001 ). Applying ZOE, only orange oil solvent significantly increased the SBS of the composite to the primary tooth dentin ( P = 0.01 ). Conclusion. To reduce the negative effects of endodontic root filling materials on the SBS of composite and primary tooth dentin, ethanol is a suitable solvent when Metapex is used, while orange oil might be a better choice than ethanol when applying ZOE.

Characterization of Multiscale Interactions Between High Intensity Focused Ultrasound (HIFU) and Tooth Dentin: Effect on Matrix-Metalloproteinases, Bacterial Biofilms and Biological Properties

Aim: To characterize multiscale interactions between high intensity focused ultrasound (HIFU) and dentin collagen and associated matrix-metalloproteinases; in addition, to analyse effect of HIFU on bacterial biofilms and biological properties....

Candida albicans Virulence Factors and Pathogenicity for Endodontic Infections

Candida albicans (C. albicans) is the fungus most frequently isolated from endodontic root canal infections. Although recognized by dental pulp and periradicular tissue cells that elicit immune responses, it eludes host defenses and elicits cell death. Then, C. albicans binds tooth dentin, forms biofilms, and invades dentinal tubules to resist intracanal disinfectants and endodontic treatments. Insensitive to most common medicaments, it survives sequestered within biofilms and intratubular dentin. Thus, C. albicans has been associated with cases of persistent or refractory root canal infections. Its treatment strategies may require alternative intracanal irrigants, intracanal medicaments such as chlorhexidine gel or human beta defensin-3 (HBD3), Ca-Si-based obturating materials, and microsurgical procedures.