Efficient hydrolysis of wine and grape juice anthocyanins by Malbranchea pulchella β-glucosidase immobilized on MANAE-agarose and ConA-Sepharose supports

Bioconversion of renewable lignocellulosic biomass to biofuel and value-added products is globally gaining significant importance. Lignocellulosic wastes are the most promising feedstock considering its great availability and low cost. The biomass conversion process involves mainly two steps: hydrolysis of cellulose in the lignocellulosic biomass to produce reducing sugars and fermentation of the sugars to ethanol and other bioproducts. However, sugars necessary for fermentation are trapped inside the recalcitrant structure of the lignocellulose. Hence, pretreatment of lignocellulosic wastes is always necessary to alter and/or remove the surrounding matrix of lignin and hemicelluloses in order to improve the hydrolysis of cellulose. These pretreatments cause physical and/or chemical changes in the plant biomass in order to achieve this result. Each pretreatment has a specific effect on the cellulose, hemicellulose, and lignin fraction. Thus, the pretreatment methods and conditions should be chosen according to the process configuration selected for the subsequent hydrolysis steps. In general, pretreatment methods can be classified into four categories, including physical, physicochemical, chemical, and biological pretreatment. Bioresource utilization of biopolymeric materials has now gained recent attention. Cellulose was extracted from water hyacinth by acid, alkali treatment & extracted cellulose was grafted with curcumin, pesticide, grape juice, magnetorheological fluid, and the grafted composite material was evaluated for release of respective grafted materials. In the present study, a polymer extracted from water hyacinth was evaluated for various applications. The present study would suggest the possible utilization of water hyacinth composite as the biomaterial for diverse applications.

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The substrate specificity of thermomycolase, an extracellular serine proteinase from the thermophilic fungus Malbranchea pulchella var. sulfurea

Biochemical Journal ◽

10.1042/bj1510527 ◽

1975 ◽

Vol 151 (3) ◽

pp. 527-542 ◽

Cited By ~ 17

Author(s):

K J Stevenson ◽

G M Gaucher

Keyword(s):

Amino Acid ◽

Methyl Ester ◽

Congo Red ◽

Serine Proteinase ◽

Amino Acid Residues ◽

Peptide Bonds ◽

Native Collagen ◽

Hydrophobic Binding ◽

Malbranchea Pulchella ◽

Hydrolysis Of

The specificity of thermomycolase toward glucagon and the oxidized A and B chains of insulin was investigated. Extensive digestion of glucagon occurred when conducted at pH 7.0 and 45 ° C for 40 min, whereas hydrolysis of only three peptide bonds occurred at pH 7.0 and 28 ° C for 5 min. A similar situation was observed for the oxidized B chain of insulin, which exhibited only a single major cleavage after 5 min at 25 ° C. No well-defined specificity for particular amino acid residues was evident, but ready hydrolysis of peptide bonds occurred within sequences containing non-polar residues. This endoproteinase must therefore possess an extended hydrophobic binding site for polypeptides. Thermomycolase hydrolysed acetylalanylalanylalanine methyl ester and elastin-Congo Red at 22 and 8.5 times the rate of porcine elastase respectively. A limited degradation of native collagen and significant hydrolysis of benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro were suggestive of some collagenase-like activity. No keratinase activity was apparent.

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Hydrolysis of para-substituted phenyl-.BETA.-D-xylosides by .BETA.-xylosidase from Malbranchea pulchella var. sulfurea No. 48.

Agricultural and Biological Chemistry ◽

10.1271/bbb1961.45.1603 ◽

1981 ◽

Vol 45 (7) ◽

pp. 1603-1608 ◽

Cited By ~ 1

Author(s):

Masaru MATSUO ◽

Tsuneo YASUI

Keyword(s):

Malbranchea Pulchella ◽

Hydrolysis Of

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Levels of Furaneol in Msalais Wines: A Comprehensive Overview of Multiple Stages and Pathways of Its Formation during Msalais Winemaking

Molecules ◽

10.3390/molecules24173104 ◽

2019 ◽

Vol 24 (17) ◽

pp. 3104 ◽

Cited By ~ 1

Author(s):

Li-Xia Zhu ◽

Meng-Meng Zhang ◽

Zheng Liu ◽

Yin Shi ◽

Chang-Qing Duan

Keyword(s):

High Performance ◽

Grape Juice ◽

Wine Quality ◽

Comprehensive Overview ◽

Important Indicator ◽

Grape Quality ◽

Chemical Hydrolysis ◽

Hydrolysis Of ◽

Maillard Products

4-Hydroxy-2,5-dimethyl-3(2H)-furanone (furaneol) is present in food. It has a caramel-like flavor, which affects the quality of food, and is formed via multiple pathways. Msalais is a traditional wine fermented from boiled local grape juice in Xinjiang (China). It has a strong caramel odor, which suggests high furaneol content. Furaneol formation during Msalais-making had not been investigated to date. Here, high-performance liquid chromatography and different fermentation models of Msalais-making were used to investigate the furaneol content and formation during Msalais-making. The furaneol content of Msalais is high, between 27.59 ± 0.493 mg/L and 117.6 ± 0.235 mg/L. It is formed throughout the entire Msalais-making process. The formation pathways include the Maillard reaction and chemical hydrolysis of bound furaneol during grape juice concentration; enzymatic release and/or chemical acidic hydrolysis of furaneol glucosides, and biosynthesis from Maillard products and d-fructose-1,6-diphosphate during fermentation; chemical transformation of Maillard products at room temperature (16–25 °C) and hydrolysis of furaneol glucosides during storage. Importantly, furaneol is formed by an efficient biotransformation of Maillard products. These findings suggest that furaneol content can be used as an important indicator of wine quality, and could be controlled by controlling the grape quality, grape juice concentration, fermentation, and wine storage.

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Hydrolysis of terpenyl glycosides in grape juice and other fruit juices: a review

Applied Microbiology and Biotechnology ◽

10.1007/s00253-004-1806-0 ◽

2005 ◽

Vol 67 (3) ◽

pp. 322-335 ◽

Cited By ~ 157

Author(s):

Sergi Maicas ◽

José Juan Mateo

Keyword(s):

Grape Juice ◽

Fruit Juices ◽

Hydrolysis Of

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Fine Structural Localization of Acyltransferases Involved in Lipid Synthesis in Intestinal Mucosa.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100067261 ◽

1970 ◽

Vol 28 ◽

pp. 54-55

Author(s):

R. J. Barrnett ◽

J. A. Higgins

Keyword(s):

Smooth Endoplasmic Reticulum ◽

Lipid Synthesis ◽

Mucosal Cell ◽

Structural Localization ◽

Morphological Studies ◽

Mucosal Cells ◽

Initial Appearance ◽

Sh Group ◽

Hydrolysis Of ◽

Intestinal Mucosal Cells

The main products of intestinal hydrolysis of dietary triglycerides are free fatty acids and monoglycerides. These form micelles from which the lipids are absorbed across the mucosal cell brush border. Biochemical studies have indicated that intestinal mucosal cells possess a triglyceride synthesising system, which uses monoglyceride directly as an acylacceptor as well as the system found in other tissues in which alphaglycerophosphate is the acylacceptor. The former pathway is used preferentially for the resynthesis of triglyceride from absorbed lipid, while the latter is used mainly for phospholipid synthesis. Both lipids are incorporated into chylomicrons. Morphological studies have shown that during fat absorption there is an initial appearance of fat droplets within the cisternae of the smooth endoplasmic reticulum and that these subsequently accumulate in the golgi elements from which they are released at the lateral borders of the cell as chylomicrons.We have recently developed several methods for the fine structural localization of acyltransferases dependent on the precipitation, in an electron dense form, of CoA released during the transfer of the acyl group to an acceptor, and have now applied these methods to a study of the fine structural localization of the enzymes involved in chylomicron lipid biosynthesis. These methods are based on the reduction of ferricyanide ions by the free SH group of CoA.

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Lattice imaging and pore structure of β ferric oxyhydroxide

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100108465 ◽

1978 ◽

Vol 36 (1) ◽

pp. 264-265

Author(s):

T. Baird ◽

J.R. Fryer ◽

S.T. Galbraith

Keyword(s):

Electron Microscopy ◽

Room Temperature ◽

Bulk Material ◽

Model Structure ◽

Bulk Crystals ◽

Lattice Imaging ◽

Thin Sections ◽

Ferric Oxyhydroxide ◽

Resolution Electron ◽

Hydrolysis Of

Introduction Previously we had suggested (l) that the striations observed in the pod shaped crystals of β FeOOH were an artefact of imaging in the electron microscope. Contrary to this adsorption measurements on bulk material had indicated the presence of some porosity and Gallagher (2) had proposed a model structure - based on the hollandite structure - showing the hollandite rods forming the sides of 30Å pores running the length of the crystal. Low resolution electron microscopy by Watson (3) on sectioned crystals embedded in methylmethacrylate had tended to support the existence of such pores.We have applied modern high resolution techniques to the bulk crystals and thin sections of them without confirming these earlier postulatesExperimental β FeOOH was prepared by room temperature hydrolysis of 0.01M solutions of FeCl3.6H2O, The precipitate was washed, dried in air, and embedded in Scandiplast resin. The sections were out on an LKB III Ultramicrotome to a thickness of about 500Å.

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