scholarly journals Hyaluronic Acid Reduces Bacterial Fouling and Promotes Fibroblasts’ Adhesion onto Chitosan 2D-Wound Dressings

2020 ◽  
Vol 21 (6) ◽  
pp. 2070 ◽  
Author(s):  
Ilaria Silvestro ◽  
Mariangela Lopreiato ◽  
Anna Scotto d’Abusco ◽  
Valerio Di Lisio ◽  
Andrea Martinelli ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Biofilm Formation ◽  
Transmission Rate ◽  
Wound Care ◽  
Chemical Properties ◽  
Infectious Complications ◽  
Wound Dressings ◽  
Physico Chemical ◽  
Wound Healing Activity

Wound healing is a dynamic process that can be seriously delayed by many factors including infectious complications. The development of dressings with intrinsic wound healing activity and/or releasing bioactive compounds may help with addressing such an issue. In this study, hyaluronic acid (HA) at different percentages (1–35%) was used to modify chitosan (CS) biological and physico-chemical properties in order to obtain 2D-matrices able to promote healing and protect from infection. HA incorporation in the CS matrix decreased film transparency and homogeneity, but improved film water uptake and surface wettability. The water vapor transmission rate (WVTR) increased up to a 5% HA content, where it reached the highest value (672 g/m2 day), and decreased for higher HA contents. At all of the tested HA concentrations, HA affected mechanical properties providing matrices more flexible than pure CS with benefit for wound care. Pure CS films permitted S. epidermidis adhesion and biofilm formation. That was not true for CS/HA matrices, where HA at concentrations equal to or greater than 5% was able to avoid S. epidermidis adhesion. Fibroblasts adhesion also took benefit from the HA presence in the film, especially at 5% content, where the best adhesion and proliferation was found.

Evaluation of gelatin-hyaluronic acid composite hydrogels for accelerating wound healing

2017 ◽  
Vol 31 (10) ◽  
pp. 1380-1390 ◽  
Author(s):  
Song Wu ◽  
Liang Deng ◽  
Hanson Hsia ◽  
Kai Xu ◽  
Yu He ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Water Vapor ◽  
Transmission Rate ◽  
Wound Dressings ◽  
Water Evaporation ◽  
Water Vapor Transmission Rate ◽  
Fluid Uptake ◽  
Water Vapor Transmission

Excellent wound dressings maintain a warm and moist environment, thus accelerating wound healing. In this study, we cross-linked gelatin and hyaluronic acid with ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride in different ratios (gelatin/hyaluronic acid = 8:2, gelatin/hyaluronic acid = 5:5, gelatin/hyaluronic acid = 2:8), and explored the effects and mechanisms of gelatinhyaluronic acid hydrogels on wound healing. This was done by examining dressing properties, such as fluid uptake ability, water vapor transmission rate, and the rate of water evaporation. We further verified biological function by using in vitro and in vivo wound models. The hydrogels display appropriate fluid uptake ability and good water vapor transmission rate and rate of water evaporation all of which can provide an adequate moisture environment for wound healing. Cell cytotoxicity and proliferation tests show that the hydrogels have no cytotoxicity, furthermore, gelatin/hyaluronic acid = 8:2 hydrogels have the potential to promote cell proliferation. Animal wound models demonstrate that the hydrogels can effectively promote wound healing in vivo, in particular, the gelatin/hyaluronic acid = 8:2 group which promoted the most rapid healing. Accordingly, gelatin-hyaluronic acid dressings, especially the gelatin/hyaluronic acid = 8:2 hydrogels, have a promising outlook for clinical applications in wound healing.

scholarly journals Hyaluronic Acid: The Influence of Molecular Weight on Structural, Physical, Physico-Chemical, and Degradable Properties of Biopolymer

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1800 ◽  
Author(s):  
Petr Snetkov ◽  
Kseniia Zakharova ◽  
Svetlana Morozkina ◽  
Roman Olekhnovich ◽  
Mayya Uspenskaya
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Therapeutic Effect ◽  
Chemical Properties ◽  
Wound Dressings ◽  
Present Review ◽  
Joint Fluid ◽  
Molecular Weights ◽  
Polymeric Scaffolds ◽  
Physico Chemical

Hyaluronic acid, as a natural linear polysaccharide, has attracted researchers’ attention from its initial detection and isolation from tissues in 1934 until the present day. Due to biocompatibility and a high biodegradation of hyaluronic acid, it finds wide application in bioengineering and biomedicine: from biorevitalizing skin cosmetics and endoprostheses of joint fluid to polymeric scaffolds and wound dressings. However, the main properties of aqueous polysaccharide solutions with different molecular weights are different. Moreover, the therapeutic effect of hyaluronic acid-based preparations directly depends on the molecular weight of the biopolymer. The present review collects the information about relations between the molecular weight of hyaluronic acid and its original properties. Particular emphasis is placed on the structural, physical and physico-chemical properties of hyaluronic acid in water solutions, as well as their degradability.

scholarly journals New Hyaluronic Acid/Polyethylene Oxide-Based Electrospun Nanofibers: Design, Characterization and In Vitro Biological Evaluation

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1291
Author(s):  
Oana Maria Ionescu ◽  
Arn Mignon ◽  
Andreea Teodora Iacob ◽  
Natalia Simionescu ◽  
Luminita Georgeta Confederat ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Ethylene Oxide ◽  
Transmission Rate ◽  
Biological Evaluation ◽  
Wound Dressings ◽  
Calendula Officinalis ◽  
Active Components ◽  
Poly Ethylene Oxide ◽  
Nanofiber Membranes ◽  
Poly Ethylene

Natural compounds have been used as wound-healing promoters and are also present in today’s clinical proceedings. In this research, different natural active components such as propolis, Manuka honey, insulin, L-arginine, and Calendula officinalis infusion were included into hyaluronic acid/poly(ethylene)oxide-based electrospun nanofiber membranes to design innovative wound-dressing biomaterials. Morphology and average fiber diameter were analyzed by scanning electron microscopy. Chemical composition was proved by Fourier transform infrared spectroscopy, which indicated successful incorporation of the active components. The nanofiber membranes with propolis and Calendula officinalis showed best antioxidant activity, cytocompatibility, and antimicrobial properties against pathogen strains Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa and had an average diameter of 217 ± 19 nm with smooth surface aspect. Water vapor transmission rate was in agreement with the range suitable for preventing infections or wound dehydration (~5000 g/m2 24 h). Therefore, the developed hyaluronic acid/poly(ethylene)oxide nanofibers with additional natural components showed favorable features for clinical use as wound dressings.

Identifying New Pathways and Targets for Wound Healing and Therapeutics from Natural Sources

2021 ◽  
Vol 18 ◽  
Author(s):  
Xinchi Feng ◽  
Jinsong Hao
Keyword(s):  
Wound Healing ◽  
Molecular Mechanisms ◽  
Chronic Wounds ◽  
Wound Care ◽  
Healing Process ◽  
Unmet Need ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Natural Sources ◽  
Viable Approach

: Chronic wounds remain a significant public problem and the development of wound treatments has been a research focus for the past few decades. Despite advances in the products derived from endogenous substances involved in a wound healing process (e.g. growth factors, stem cells, and extracellular matrix), effective and safe wound therapeutics are still limited. There is an unmet need to develop new therapeutics. Various new pathways and targets have been identified and could become a molecular target in designing novel wound agents. Importantly, many existing drugs that target these newly identified pathways could be repositioned for wound therapy, which will facilitate fast translation of research findings to clinical applications. This review discusses the newly identified pathways/targets and their potential uses in the development of wound therapeutics. Some herbs and amphibian skins have been traditionally used for wound repairs and their active ingredients have been found to act in these new pathways. Hence, screening these natural products for novel wound therapeutics remains a viable approach. The outcomes of wound care using natural wound therapeutics could be improved if we can better understand their cellular and molecular mechanisms and fabricate them in appropriate formulations, such as using novel wound dressings and nano-engineered materials. Therefore, we also provide an update on the advances in the wound therapeutics from natural sources. Overall, this review offers new insights into novel wound therapeutics.

scholarly journals Preparation of Foam Dressings Based on Gelatin, Hyaluronic Acid, and Carboxymethyl Chitosan Containing Fibroblast Growth Factor-7 for Dermal Regeneration

Polymers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 3279
Author(s):  
Longhao Jin ◽  
Sun-Jung Yoon ◽  
Dae Hoon Lee ◽  
Yun Chang Pyun ◽  
Woo Youp Kim ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Hyaluronic Acid ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Wound Dressing ◽  
Transmission Rate ◽  
Carboxymethyl Chitosan ◽  
Amide Bond ◽  
Fibroblast Growth

Wound recovery close to the function of the native skin is the goal of wound healing. In this study, we prepared foam dressings (FDs; 2-GHC-FD-1–9, 5-GHC-FD-1–9, and 10-GHC-FD-1–9) composed of various concentrations of gelatin, hyaluronic acid, and carboxymethyl chitosan, which are chemically interconnected through amide bond formation, for evaluating wound healing. Tensile and cell proliferation tests showed that 2-GHC-FD-1–9 are suitable for wound dressing. For further evaluation, three types of FDs, 2-GHC-FD-1, 2-GHC-FD-4, and 2-GHC-FD-8 were chosen. The results of animal intradermal reactivity, water vapor transmission rate, and absorption rate of the three FDs indicated that 2-GHC-FD-8 is the most appropriate scaffold for wound healing. For wound healing acceleration, various concentrations of fibroblast growth factor-7 (FGF-7) was soaked in 2-GHC-FD-8 (2-GHC-FD-8/F1-6) and evaluated by using scanning electron microscopy, cell proliferation, release behavior, and in vivo animal tests. The FDs showed interconnected porous structures, increased cell proliferation until 8.0 × 10−11 M, controlled release with initial burst within 1 h, and sustained release for 48 h. The results of the animal test showed an appropriate concentration of FGF-7 for wound healing. In addition, 2-GHC-FD-8 is a suitable scaffold for wound healing. Therefore, we suggest that 2-GHC-FD-8/F3 is a useful wound dressing for accelerating wound healing.

scholarly journals Evaluation of wound healing activity of alpha mangostin ointment in rats

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1080-1087
Author(s):  
Sasikala Chinnappan ◽  
Venkatalakshmi Ranganathan ◽  
Jithendra Panneerselvam ◽  
Barani Karikalan ◽  
Thivashini vasanthan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Wound Model ◽  
Wound Size ◽  
Group I ◽  
Significant Difference ◽  
Histopathological Studies ◽  
Wound Healing Activity

This study was aimed to evaluate the wound healing effects of alpha mangostin ointment using excision wound model. Twenty rats were divided into four groups of five rats each; group I was treated with ointment base (control), whereas group II, group III and group IV were treated with 10% (w/w) povidone-iodine (standard), alpha mangostin 1% (w/w), and alpha mangostin 2 % (w/w) respectively for wound healing evaluations. The size of the wound area was measured, and the reduction in the wound size was calculated, and the tissues examined histologically. The significant difference in the wound size reduction between the control and treated group (p<0.05) was observed in wound healing activity. Histopathological studies showed a lesser number of chronic inflammatory cells, lesser oedema and increased collagenisation in the test than control. This study showed that the alpha mangostin topical ointment could be a promising candidate for the betterment of wound care.

scholarly journals Scaffolds for Wound Healing Applications

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2010 ◽  
Author(s):  
Irina Negut ◽  
Gabriela Dorcioman ◽  
Valentina Grumezescu
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Water Vapor Permeability ◽  
Cellular Migration ◽  
Wound Dressings ◽  
Vapor Permeability ◽  
Therapeutic Effects ◽  
Microbial Invasion ◽  
Polymeric Biomaterials ◽  
Hybrid Biomaterials

In order to overcome the shortcomings related to unspecific and partially efficient conventional wound dressings, impressive efforts are oriented in the development and evaluation of new and effective platforms for wound healing applications. In situ formed wound dressings provide several advantages, including proper adaptability for wound bed microstructure and architecture, facile application, patient compliance and enhanced therapeutic effects. Natural or synthetic, composite or hybrid biomaterials represent suitable candidates for accelerated wound healing, by providing proper air and water vapor permeability, structure for macro- and microcirculation, support for cellular migration and proliferation, protection against microbial invasion and external contamination. Besides being the most promising choice for wound care applications, polymeric biomaterials (either from natural or synthetic sources) may exhibit intrinsic wound healing properties. Several nanotechnology-derived biomaterials proved great potential for wound healing applications, including micro- and nanoparticulate systems, fibrous scaffolds, and hydrogels. The present paper comprises the most recent data on modern and performant strategies for effective wound healing.

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