Levan from a new isolated Bacillus subtilis AF17: Purification, structural analysis and antioxidant activities

2020 ◽  
Vol 144 ◽  
pp. 316-324 ◽  
Author(s):  
Amir Bouallegue ◽  
Angela Casillo ◽  
Fatma Chaari ◽  
Annalisa La Gatta ◽  
Rosa Lanzetta ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Structural Analysis ◽  
Antioxidant Activities

scholarly journals Phytochemical Screening, Antimicrobial and Antioxidant Activities of Sonchus Oleraceus l (Leaves) Extracts

2020 ◽  
pp. 1-4
Author(s):  
Hatim MY Hamadnalla ◽  
◽  
Mahdi Abd Elmageed Mohammed ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Sonchus Oleraceus

The objective of this study to examine phytochemical analysis of sonchus oleraceus l, (asteraceae) (moliata) extracts which were extracted by four solvents, petroleum ether, chloroform, methanol and distill water and to assess their potential antibacterial against four standard bacteria gram-positive and gramnegative (bacillus subtilis staphylococcus aureu, salmonella typhi, and pscudomona aeruginosa) and antioxidant activities. (moliata) extracts using cold maceration method to extract sonchus oleraceus l, (asteraceae) locally known as (moliata) extracts, where dpph assay and paper disc diffusion assay were employed to evaluate the antioxidant and antibacterial activities respectively. The results showed that tannins, triterpenes, a sterols, flavonoids and cardiac glycosides compounds were present in the sonchus oleraceus l, (asteraceae) lives (moliata) extracts. The methanol extract of leaves showed high antioxidant potential (77± 0.03 % ) and chloroform showed low antioxidant potential activity (4±0.09) compare with propyl gallate as standard (93± 0.010 %) against dpph. Chloroform extract was effective and give moderate zone of inhibition against staphylococcus aureus. Methanol extract was effective and showed moderate zone of inhibition against staphylococcus aureus, bacillus subtilis, salmonella typhi and pseudomonas aeruginosa.

Subtilosin A, a New Antibiotic Peptide Produced by Bacillus subtilis 168: Isolation, Structural Analysis, and Biogenesis1

1985 ◽  
Vol 98 (3) ◽  
pp. 585-603 ◽  
Author(s):  
Katsuhiko BABASAKI ◽  
Toshifumi TAKAO ◽  
Yasutsugu SHIMONISHI ◽  
Kiyoshi KURAHASHI
Keyword(s):  
Bacillus Subtilis ◽  
Structural Analysis ◽  
Bacillus Subtilis 168 ◽  
Antibiotic Peptide

scholarly journals ANTIBACTERIAL ACTIVITY AGAINST BACILLUS SUBTILIS AND ANTIOXIDANT PROPERTIES OF METHANOL EXTRACTS FROM GARCINIA LATISSIMA MIQ. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 24 ◽  
Author(s):  
Neneng Siti Silfi Ambarwati ◽  
Berna Elya ◽  
Amarila Malik ◽  
Muhammad Hanafi ◽  
Hanita Omar
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Crude Extract ◽  
Inhibitory Concentration ◽  
Antioxidant Activities ◽  
Wide Spectrum ◽  
Antioxidant Properties ◽  
Antibacterial Activities ◽  
Positive Control ◽  
Inhibition Zones

Objective: The objective of this study was to identify fractions with the highest antibacterial activity against Bacillus subtilis and to determineantioxidant activities and establish the chromatographic fractions as candidate antibacterial and antioxidant agents.Methods: Extracts were fractionated using column chromatography, and antibacterial activities were assayed by the analyses of inhibition zones andbioautography, as well as by broth microdilution techniques. Antioxidant activities were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH)assay.Results: The strongest antibacterial activity against B. subtilis (ATCC 6633) was observed with fractions B and C obtained in this research, witha minimum inhibitory concentration value of 312.5 μg/mL. The effective percentage (EP) value of crude extract at 10 μg/mL was 29.47±2.01%.Fractions C and D had greater EP values than the crude extract, whereas fraction D had the highest scavenging activity against DPPH free radicals(37.73±1.44%) when used at 10 μg/mL. The half effective concentration of the extract was 23.40 μg/mL, whereas that of the most active fraction Dwas 19.38 μg/mL and quercetin as positive control was 3.72 μg/mL.Conclusion: The present data confirm that fractions of methanol extract from Garcinia latissima Miq. leaves possess antibacterial and antioxidantactivities. These observations may facilitate the development of antimicrobial phytomedicines with a wide spectrum of activities and standardizedantioxidant properties.

Structural analysis and antioxidant activities of polysaccharide isolated from Jinqian mushroom

2014 ◽  
Vol 64 ◽  
pp. 63-68 ◽  
Author(s):  
Yong Liu ◽  
Yi-Qun Du ◽  
Jun-Hui Wang ◽  
Xue-Qiang Zha ◽  
Jian-Bo Zhang
Keyword(s):  
Structural Analysis ◽  
Antioxidant Activities

High pressure treated Bacillus subtilis spores — Structural analysis by means of synchrotron and laboratory based soft X-ray microscopy

2015 ◽  
Vol 29 ◽  
pp. 134-142 ◽  
Author(s):  
Christian Seim ◽  
Kai Reineke ◽  
Stephan Werner ◽  
Aurélie Dehlinger ◽  
Herbert Legall ◽  
...  
Keyword(s):  
High Pressure ◽  
Bacillus Subtilis ◽  
Structural Analysis ◽  
X Ray

Preparation and Antioxidant Activities of Peanut (Arachin conarachin L.) Protein Peptides by Bacillus Subtilis Solid State Fermentation Method

2014 ◽  
Vol 887-888 ◽  
pp. 601-604
Author(s):  
Li Na Yu ◽  
Jian Xiong Feng ◽  
Qiang Qiang Ming ◽  
Qing Li Yang ◽  
Chu Shu Zhang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Free Radicals ◽  
Solid State ◽  
Solid State Fermentation ◽  
Antioxidant Activities ◽  
Nitrogen Concentration ◽  
Bacterial Suspension ◽  
Soluble Nitrogen ◽  
Fermentation Method ◽  
Nutrient Salt

The objective of this work is to study on preparation of peanut protein peptide by bacillus subtilis solid state fermentation method. Results indicated that soluble nitrogen concentration of protein peptide could reach 122.86mg/mL, 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and hydroxyl free radicals scavenging rates were 85.22% and 84.88%, respectively, under the following optimum conditions: nutrient salt solution of 16.3mL, bacterial suspension volume of 3.8mL, temperature of 45°C, and time of 41h. IC50values for scavenging of DPPH, hydroxyl and superoxide anion free radicals rates, iron and copper ion chelating rates, inhibition rate of anti-lipid peroxidation were 2.88mg/mL, 4.63mg/mL, 1.22mg/mL, 0.04mg/mL, 0.94mg/mL, 3.78mg/mL, respectively. When absorbance value of iron and molybdenum reduction capacities was 0.5, the required protein peptide concentrations were 7.28mg/mL and 1.85mg/mL, respectively.

Structural analysis and antioxidant activities of neutral polysaccharide isolated from Epimedium koreanum Nakai

2018 ◽  
Vol 196 ◽  
pp. 246-253 ◽  
Author(s):  
Bo Li ◽  
Ning Zhang ◽  
Dong Xue Wang ◽  
Lili Jiao ◽  
Ying Tan ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Antioxidant Activities ◽  
Epimedium Koreanum Nakai
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