Silk sericin application increases bone morphogenic protein-2/4 expression via a toll-like receptor-mediated pathway

This study investigated the effects of the ionic dissolution products of NovaBone® on osteoblastic proliferation and cell cycle regulation. MG63 osteoblast-like cells were cultured in NovaBone®-conditioned Dulbecco's Modified Eagle's Medium (DMEM) or control DMEM for 10 days. The concentration of silicon ions was significantly higher in NovaBone®-conditioned DMEM than control DMEM. MG63 cells cultured in NovaBone®-conditioned DMEM exhibited greater proliferation on days 1 and 4 than control cells. There were increased proportions of Novabone®-conditioned DMEM-cultured cells in the S and G2/M phases, and decreased proportions in the G0/G1 phase on days 1 and 4 versus control cells, while no differences were observed on days 7 and 10 between the two groups. Bone morphogenic protein 2 production increased in both groups, but was significantly higher for the NovaBone®-conditioned DMEM-cultured cells on day 10 compared with the controls. In conclusion, the NovaBone® ionic dissolution products, particularly the silicon ions, promoted proliferation of MG63 osteoblast-like cells in vitro via influences on the cell cycle.

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Granulin epithelin precursor: a bone morphogenic protein 2‐inducible growth factor that activates Erk1/2 signaling and JunB transcription factor in chondrogenesis

The FASEB Journal ◽

10.1096/fj.09-144659 ◽

2010 ◽

Vol 24 (6) ◽

pp. 1879-1892 ◽

Cited By ~ 79

Author(s):

Jian Q. Feng ◽

Feng‐Jin Guo ◽

Bai‐Chun Jiang ◽

Yan Zhang ◽

Sally Frenkel ◽

...

Keyword(s):

Transcription Factor ◽

Growth Factor ◽

Bone Morphogenic Protein ◽

Bone Morphogenic Protein 2

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Complications Associated With the Use of Recombinant Human Bone Morphogenic Protein–2 in Ridge Augmentation: A Case Report

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-17-00101 ◽

2017 ◽

Vol 43 (5) ◽

pp. 351-359 ◽

Cited By ~ 3

Author(s):

Panagiotis Dragonas ◽

Charles Palin ◽

Saba Khan ◽

Praveen K. Gajendrareddy ◽

Whitney D. Weiner

Keyword(s):

Case Report ◽

Bone Morphogenic Protein ◽

Human Bone ◽

Therapy Outcomes ◽

Bone Maturation ◽

Ridge Augmentation ◽

Implant Placement ◽

Implant Dentistry ◽

Bone Morphogenic Protein 2 ◽

Recombinant Human Bone

This case report aims to describe in detail a complication associated with resorption of regenerated bone following implant placement and ridge augmentation using recombinant human bone morphogenic protein–2 (rhBMP-2) in combination with allograft and xenograft. Bilateral maxillary sinus and ridge augmentation procedures were completed using rhBMP-2 combined with allograft and xenograft. Five months later, significant bone augmentation was achieved, which allowed for the placement of 4 implants. Upon stage 2 surgery, significant dehiscence was noted in all implants. Treatment steps to address this complication included implant removal, guided bone regeneration with xenograft only, and placement of new implants followed by soft-tissue grafting. At the time of publication, this patient is status 1½ years post case completion with maintenance of therapy outcomes. Off-label use of rhBMP-2 has gained significant acceptance in implant dentistry. However, there is limited evidence regarding the bone maturation process when rhBMP-2 is combined with other biomaterials. More research may be needed regarding the timing and process of bone healing in the presence of rhBMP-2, in an effort to avoid surgical complications.

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The effect of the delivery of vascular endothelial growth factor and bone morphogenic protein-2 to osteoprogenitor cell populations on bone formation

Biomaterials ◽

10.1016/j.biomaterials.2009.10.059 ◽

2010 ◽

Vol 31 (6) ◽

pp. 1242-1250 ◽

Cited By ~ 164

Author(s):

Janos M. Kanczler ◽

Patrick J. Ginty ◽

Lisa White ◽

Nicholas M.P. Clarke ◽

Steven M. Howdle ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Bone Formation ◽

Bone Morphogenic Protein ◽

Cell Populations ◽

Vascular Endothelial ◽

Osteoprogenitor Cell ◽

Endothelial Growth Factor ◽

Bone Morphogenic Protein 2

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The potentiation of Mangifera casturi bark extract on interleukin- 1β and bone morphogenic protein-2 expressions during bone remodeling after tooth extraction

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v50.i1.p36-42 ◽

2017 ◽

Vol 50 (1) ◽

pp. 36

Author(s):

Bayu Indra Sukmana ◽

Theresia Indah Budhy ◽

I Gusti Aju Wahju Ardani

Keyword(s):

Bone Remodeling ◽

Tooth Extraction ◽

Alveolar Bone ◽

Interleukin 1 ◽

Bone Morphogenic Protein ◽

Bark Extract ◽

Control Group ◽

Tooth Decay ◽

Significant Difference ◽

Bone Morphogenic Protein 2

Background: The main oral health problem in Indonesia is the high number of tooth decay. Tooth extraction is the treatment often received by patients who experience tooth decay and the wound caused by alveolar bone resorption. Bark of Mangifera casturi has been studied and proven to contain secondary metabolite which has the ability to increase osteoblast’s activity and suppress osteoclast’s activity. Purpose: The purpose of this study was to analyze interleukin-1 beta (IL-1β) and bone morphogenic protein-2 (BMP-2) activities during bone remodeling after Mangifera casturi’s bark extract treatment. Method: This study was laboratory experimental research with randomized post-test only control group design. The Mangifera casturi bark was extracted using 96% ethanol maceration and n-hexane fractionation. This study used 40 male Wistar rats which are divided into 4 groups and the tooth extraction was performed on the rats’ right mandible incisive tooth. The four groups consisted of 6.35%, 12.7%, 25.4% extract treatment group, and a control group. Wistar’s mandibles were decapitated on the 7th and 14th day after extraction. Antibody staining on preparations for the examination of IL-1β and BMP-2 expressions was done using immunohistochemistry. Result: There was a significant difference of IL-1β and BMP-2 expressions in 6,35%, 12,7%, and 25,4% treatment groups compared to control group with p<0.05. Conclusion: Mangifera casturi’s bark extract was able to suppress the IL-1β expression and increase the BMP-2 expression during bone remodeling after tooth extraction.

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