Background:
Lately, the employment of nano-carriers has been known as an optimistic means of drug and
vaccine delivery.
Objective:
Nano vaccines are a novel tier of vaccines that can develop humoral and cellular immune responses and can be
introduced as a practical and secure nano vaccine candidate the prevention of diseases. The purpose of this study was to
accomplish and use biodegradable nano-carriers for the synthesis of pentavalent vaccine and immunogenicity evaluation in
the animal models.
The PLGA nanoparticle was prepared and modified with chitosan nanoparticle. Nano-carrier PLGA-chitosan was loaded by
the DTP-HepB-Hib antigens and confirmed by DLS, SEM, TEM and FT-IR then the in vitro loading and release were
evaluated. The toxicity was assessed by the MTT method in the Hec293 cells. The antigenicity evaluation and
histopathological study were conducted by injection of new nano pentavalent vaccines in the BALB/c mice and the immune
response was measured in the serum samples through an indirect ELISA method.
Results:
Conclusions drawn from the current study exhibited the plausible ability of nano-carrier to deliver vaccine. A
notable increase was shown in total IgG and IgM antibodies examined on mice vaccinated with new nano vaccines. The
histopathological study in the treated mice showed no toxicity in vital organs of mice.
Conclusion:
The engineered vaccine delivery system showed the ability to induce robust immune responses and also the
suitability features of the PLGA-chitosan as a promising carrier to improve vaccine delivery.
PLGA nanoparticle-mediated delivery of tumor antigenic peptides elicits effective immune responses