Isolation and identification of potential probiotic Lactobacillus species from feces of infants in southwest Iran

Abstract Objectives Healthy vaginal microbiota is mainly dominated by Lactobacillus species namely L. crispatus, L. gasseri, L. jensenii, and L. iners. Lactobacilli are thought to play an important role in the prevention of urogenital infections, and Lactobacillus probiotics to restore and/or maintain vaginal health has been advocated. These can interfere with the adherence, growth, and colonization by uropathogenic bacteria, thus reducing the risk of urinary tract infection (UTI). This study aims to isolate and evaluate the susceptibility of healthy vaginal and probiotic Lactobacillus spp. to urinary antibiotics. Materials and methods A total of 50 premenopausal, nonmenstruating women with no symptoms of vaginal infection or UTI or antimicrobial use in the past 2 weeks were enrolled. Two high vaginal swabs were collected for Nugent’s scoring and anaerobic culture. Colonies yielding gram-positive rods were confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Lactobacillus strains in a vaginal probiotic tablet were also isolated, and the sensitivity of both sets to urinary antibiotics was determined. Results A total of 29 Lactobacillus spp. were isolated, including L. crispatus (15), L. gasseri (5), L. vaginalis (4), L. paracasei (2), L. curvatus (1), L. fermentum (1), and L. paraplantarum (1). All strains were susceptible to nitrofurantoin and resistant to norfloxacin, gentamicin, and cotrimoxazole (except L. paracasei). The probiotic strains were pan-sensitive. Conclusions Prophylactic antibiotics are capable of eliminating the normal vaginal inhabitants, which may increase the probability of UTI. The administration of vaginal probiotics as an alternate or multidrug therapy can restore vaginal microbiota and help prevent recurrent UTI.

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Highly Heterogeneous Probiotic Lactobacillus Species in Healthy Iranians with Low Functional Activities

PLoS ONE ◽

10.1371/journal.pone.0144467 ◽

2015 ◽

Vol 10 (12) ◽

pp. e0144467 ◽

Cited By ~ 18

Author(s):

Mahdi Rohani ◽

Nasrin Noohi ◽

Malihe Talebi ◽

Mohammad Katouli ◽

Mohammad R. Pourshafie

Keyword(s):

Lactobacillus Species ◽

Functional Activities ◽

Probiotic Lactobacillus

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Implementation of a Non-Invasive Bioprospecting Protocol for Isolation of Lactobacillus from Feces of Hens Under Foraging Conditions

Ingeniería y Ciencia ◽

10.17230/ingciencia.14.28.4 ◽

2018 ◽

Vol 14 (28) ◽

pp. 93-111

Author(s):

Simón Robledo-Cardona ◽

Sabina Ramírez-Hincapié ◽

Javier Correa-Álvarez

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Lactobacillus Species ◽

Rrna Gene ◽

Isolation And Identification ◽

Maldi Tof Ms ◽

Non Invasive ◽

Maldi Tof ◽

Isolation And Characterization ◽

Tof Ms

In animal production, probiotics seek to replace the use of antibiotics, while diminishing mortality and morbidity rates to raise productivity. Probiotics constitute a natural alternative that, in contrast with antibiotics, neither produces pathogen resistance, nor leaves chemical residues in the final product. Several bacteria, including some belonging to the genus Lactobacillus have been described as probiotics with high potential. A non-invasive bioprospecting protocol aimed for the isolation and characterization of lactobacilli from chicken feces was established. Fecal samples were collected from the ground. These were diluted and cultured in LAB selective medium. Colonies were identified by three methods: Gram stain, MALDI-TOF MS and sequencing of 16S rRNA gene. An initial probiotic potential of lactobacilli isolates was determined via antagonism tests using five enteropathogen reference strains: Staphylococcus aureus, Enterococcus faecium, Candida albicans, Pseudomonas spp. and Salmonella spp. 24 isolates belonging to four Lactobacillus species were identified by MALDITOF MS. BLAST of 16S rRNA gene of eight randomly selected isolates, confirmed MALDI-TOF MS identification. Five of these eight isolates inhibited the growth of at least one of the pathogenic strains used, three isolates of Lactobacillus plantarum and two of Lactobacillus salivarius. Our protocol achieved 21 lactobacilli per 100 isolates performance, greatly surpassing the normal percentage of lactobacilli in chicken gut microbiome, that so, its implementation would facilitate the isolation and identification of new probiotic strains from feces.

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Probiotic Lactobacillus species Inhibitory Effect on The Growth of Oral Streptococci

International Journal of Advances in Scientific Research and Engineering ◽

10.31695/ijasre.2020.33768 ◽

2020 ◽

Vol 06 (03) ◽

pp. 125-129

Author(s):

Wah Wah Than ◽

Saw Sandar Maw ◽

Zaw Khaing Oo ◽

Reemon Htun

Keyword(s):

Inhibitory Effect ◽

Lactobacillus Species ◽

Oral Streptococci ◽

Probiotic Lactobacillus

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The probiotic Lactobacillus plantarum counteracts TNF-α-induced downregulation of SMCT1 expression and function

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00279.2010 ◽

2010 ◽

Vol 299 (4) ◽

pp. G928-G934 ◽

Cited By ~ 33

Author(s):

Alip Borthakur ◽

Arivarasu N. Anbazhagan ◽

Anoop Kumar ◽

Geetu Raheja ◽

Varsha Singh ◽

...

Keyword(s):

Mrna Expression ◽

Promoter Activity ◽

Monocarboxylate Transporter ◽

Mucosal Inflammation ◽

Lactobacillus Species ◽

Inhibitory Effects ◽

Monocarboxylate Transporter 1 ◽

Tnf Α ◽

Probiotic Lactobacillus ◽

And Function

The major short-chain fatty acid (SCFA) butyrate is produced in the colonic lumen by bacterial fermentation of dietary fiber. Butyrate serves as primary fuel for the colonocytes and also ameliorates mucosal inflammation. Disturbed energy homeostasis seen in inflamed mucosa of inflammatory bowel disease patients has been attributed to impaired absorption of butyrate. Since sodium-coupled monocarboxylate transporter 1 (SMCT1, SLC5A8) has recently been shown to play a role in Na+-coupled transport of monocarboxylates, including SCFA, such as luminal butyrate, we examined the effects of proinflammatory TNF-α on SMCT1 expression and function and potential anti-inflammatory role of probiotic Lactobacillus species in counteracting the TNF-α effects. Rat intestinal epithelial cell (IEC)-6 or human intestinal Caco-2 cells were treated with TNF-α in the presence or absence of Lactobacilli culture supernatants (CS). TNF-α treatments for 24 h dose-dependently inhibited SMCT1-mediated, Na+-dependent butyrate uptake and SMCT1 mRNA expression in IEC-6 cells and SMCT1 promoter activity in Caco-2 cells. CS of L. plantarum (LP) stimulated Na+-dependent butyrate uptake (2.5-fold, P < 0.05), SMCT1 mRNA expression, and promoter activity. Furthermore, preincubating the cells with LP-CS followed by coincubation with TNF-α significantly attenuated the inhibitory effects of TNF-α on SMCT1 function, expression, and promoter activity. In vivo, oral administration of live LP enhanced SMCT1 mRNA expression in the colonic and ileal tissues of C57BL/6 mice after 24 h. Efficacy of LP or their secreted soluble factors to stimulate SMCT1 expression and function and to counteract the inhibitory effects of TNF-α on butyrate absorption could have potential therapeutic value.

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Probiotic Lactobacillus Species Strengthen Intestinal Barrier Function and Tight Junction Integrity in Experimental Necrotizing Enterocolitis

Journal of Probiotics & Health ◽

10.4172/2329-8901.1000159 ◽

2017 ◽

Vol 05 (01) ◽

Cited By ~ 41

Author(s):

Brian P Blackwood ◽

Carrie Y Yuan ◽

Douglas R Wood ◽

Joseph D Nicolas ◽

Justyna S Grothaus ◽

...

Keyword(s):

Tight Junction ◽

Necrotizing Enterocolitis ◽

Barrier Function ◽

Intestinal Barrier ◽

Intestinal Barrier Function ◽

Lactobacillus Species ◽

Probiotic Lactobacillus

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Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

Biomolecules ◽

10.3390/biom9120752 ◽

2019 ◽

Vol 9 (12) ◽

pp. 752 ◽

Cited By ~ 12

Author(s):

Ivan Kushkevych ◽

Věra Kotrsová ◽

Dani Dordević ◽

Leona Buňková ◽

Monika Vítězová ◽

...

Keyword(s):

Hydrogen Sulfide ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Growth Parameters ◽

Sulfate Reducing Bacteria ◽

Lactobacillus Species ◽

Pcr Analysis ◽

Isolation And Identification ◽

Sulfate Reducing ◽

Reducing Bacteria

The gut microbiota is a complex component of humans that depends on diet, host genome, and lifestyle. The background: The study purpose is to find relations between nutrition, intestinal lactic acid bacteria (LAB) from various environments (human, animal intestine, and yogurt) and sulfate-reducing microbial communities in the large intestine; to compare kinetic growth parameters of LAB; and to determine their sensitivity to different concentration of hydrogen sulfide produced by intestinal sulfate-reducing bacteria. Methods: Microbiological (isolation and identification), biochemical (electrophoresis), molecular biology methods (DNA isolation and PCR analysis), and statistical processing (average and standard error calculations) of the results were used. The results: The toxicity of hydrogen sulfide produced by sulfate-reducing bacteria, the survival of lactic acid bacteria, and minimal inhibitory concentrations (MIC) were determined. The measured hydrogen sulfide sensitivity values were the same for L. paracasei and L. reuteri (MIC > 1.1 mM). In addition, L. plantarum and L. fermentum showed also a similar sensitivity (MIC > 0.45 mM) but significantly (p < 0.05) lower than L. reuteri and L. paracasei (1.1 > 0.45 mM). L. paracasei and L. reuteri are more sensitive to hydrogen sulfide than L. fermentum and L. plantarum. L. pentosus was sensitive to the extremely low concentration of H2S (MIC > 0.15 mM). Conclusions: The Lactobacillus species were significantly sensitive to hydrogen sulfide, which is a final metabolite of intestinal sulfate-reducing bacteria. The results are definitely helpful for a better understanding of complicated interaction among intestinal microbiota and nutrition.

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