scholarly journals In vitro cytotoxicity MTT assay in THP-1 cell lines study of crude extract of herbal mixtures (HS1 and HS2) used in the treatment of tuberculosis

2021 ◽  
Vol 39 ◽  
pp. S83
Author(s):  
Tomar Jyoti ◽  
Vijaylatha Rastogi ◽  
Tarun Patni
Keyword(s):  
Cell Lines ◽  
Mtt Assay ◽  
Crude Extract ◽  
In Vitro Cytotoxicity ◽  
Herbal Mixtures

scholarly journals Evaluation of Cytotoxic Activity in Ethanolic Extract of Cucumis melo (L). fruit

2020 ◽  
Vol 2 (1) ◽  
pp. 70
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Hepg2 Cells ◽  
Cucumis Melo ◽  
Ethanolic Extract ◽  
In Vitro Cytotoxicity ◽  
Cucumis Melo L

Cytotoxicity is the quality of being toxic to cells, and in vitro cytotoxicity testing procedures reduce the use of laboratory animals. The present study was designed to investigate the cytotoxic activity of the Cucumis melo (L) fruit against HepG2 cell lines. To prepare the extract, fresh pulps of Cucumis melo fruit was chopped into pieces and dried at room temperature for 24 hours. 10 g of the dried fruit powder was successively extracted with 100 ml of ethanol using Soxhlet apparatus and filtered through Whatman No 1 filter paper. The cytotoxic activity for cancer cell lines was evaluated by MTT assay. The in vitro cytotoxicity of different concentrations (18.75 - 300μg/mL) of the ethanolic extract of Cucumis melo fruit was evaluated by the MTT assay. The IC50 value is measured by the concentration of extract, causing 50% growth inhibition of cancer cells. The results indicated that the cytotoxic effect of the ethanolic extract of Cucumis melo fruit against HepG2 cells is dose-dependent. At low concentrations, the extract was found to be less toxic towards the HepG2 cells, whereas, at higher concentrations, the toxicity was increased. The concentration at 201.5 µg / ml was found to be an effective dose because, at this concentration, it exhibited 50 % cytotoxicity against HepG2 cells. This work revealed the potentials of ethanolic extract of Cucumis melo fruit as a cytotoxic agent against liver cancer cell lines. The plant can be further screened against various diseases using toxicity models in order to find out its unexplored efficacy.

Synthesis and Cytotoxicity of New Mannich Bases of 6-[3-(3,4,5-Trimetoxyphenyl)-2- propenoyl]-2(3H)-Benzoxazolone

2020 ◽  
Vol 17 (4) ◽  
pp. 512-517
Author(s):  
Ognyan Ivanov Petrov ◽  
Yordanka Borisova Ivanova ◽  
Mariana Stefanova Gerova ◽  
Georgi Tsvetanov Momekov
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Biological Activities ◽  
Human Tumor ◽  
Mannich Bases ◽  
In Vitro Cytotoxicity ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines

Background: Chemotherapy is one of the mainstays of cancer treatment, despite the serious side effects of the clinically available anticancer drugs. In recent years increasing attention has been directed towards novel agents with improved efficacy and selectivity. Compounds with chalcone backbone have been reported to possess various biological activities such as anticancer, antimicrobial, anti-inflammatory, analgesic, antioxidant, etc. It was reported that aminomethylation of hydroxy chalcones to the corresponding Mannich bases increased their cytotoxicity. In this context, our interest has been focused on the design and synthesis of the so-called multi-target molecules, containing two or more pharmacophore fragments. Methods: A series of Mannich bases were synthesized by the reaction between 6-[3-(3,4,5- trimethoxyphenyl)-2-propenoyl]-2(3Н)-benzoxazolone, formaldehyde, and a secondary amine. The structures of the compounds were confirmed by elemental analysis, IR and NMR spectra. The new Mannich bases were evaluated for their in vitro cytotoxicity against a panel of human tumor cell lines, including BV-173, SKW-3, K-562, HL-60, HD-MY-Z and MDA-MB-231. The effects of selected compounds on the cellular levels of glutathione (GSH) were determined. Results: The new compounds 4a-e exhibited concentration-dependent cytotoxic effects at micromolar concentrations in MTT-dye reduction assay against a panel of human tumor cell lines, similar to those of starting chalcone 3. The tested agents led to concentration - dependent depletion of cellular GSH levels, whereby the effects of the chalcone prototype 3 and its Mannich base-derivatives were comparable. Conclusion: The highest chemosensitivity to the tested compounds was observed in BV- 173followed by SKW-3 and HL-60 cell lines.

scholarly journals In vitro cytotoxicity of GO–DOx on FaDu squamous carcinoma cell lines

2018 ◽  
Vol Volume 13 ◽  
pp. 107-111 ◽  
Author(s):  
Manjri Singh ◽  
Parul Gupta ◽  
Richa Baronia ◽  
Priti Singh ◽  
Stalin Karuppiah ◽  
...  
Keyword(s):  
Cell Lines ◽  
Carcinoma Cell ◽  
Squamous Carcinoma ◽  
In Vitro Cytotoxicity ◽  
Carcinoma Cell Lines ◽  
Squamous Carcinoma Cell

scholarly journals The cytotoxic activity of pine needles ethanolic extract of Pinus merkusii on HeLa cell lines

2021 ◽  
Vol 33 ◽  
pp. 03001
Author(s):  
Annise Proboningrat ◽  
Amaq Fadholly ◽  
Sri Agus Sudjarwo ◽  
Fedik Abdul Rantam ◽  
Agung Budianto Achmad
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Anticancer Agent ◽  
Ethanolic Extract ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Lines ◽  
Cytotoxicity Assessment ◽  
Pinus Merkusii

Several efforts have been made to discover new anticancer agents based on natural ingredients. Meanwhile, previous studies have shown that different Pine genus species exhibit cytotoxic activity against various types of cancer cells. This plant is rich in phenolic compounds, especially procyanidins, flavonoids, and phenolic acids. Therefore, this study aims to investigate the in vitro cytotoxicity of Pinus merkusii needles extract on HeLa cancer cell lines. The cytotoxicity assessment was measured using MTT assay and expressed as IC50 value. The results showed that the ethanolic extract poses a dose and time-dependent cytotoxic activity with an IC50 value of 542.5 µg/ml at 48 hours of incubation. Based on this result, Pinus merkusii needles’ ethanolic extract has the potential of a novel candidate for an anticancer agent.

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