scholarly journals Evaluation of Cytotoxic Activity in Ethanolic Extract of Cucumis melo (L). fruit

2020 ◽  
Vol 2 (1) ◽  
pp. 70
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Hepg2 Cells ◽  
Cucumis Melo ◽  
Ethanolic Extract ◽  
In Vitro Cytotoxicity ◽  
Cucumis Melo L

Cytotoxicity is the quality of being toxic to cells, and in vitro cytotoxicity testing procedures reduce the use of laboratory animals. The present study was designed to investigate the cytotoxic activity of the Cucumis melo (L) fruit against HepG2 cell lines. To prepare the extract, fresh pulps of Cucumis melo fruit was chopped into pieces and dried at room temperature for 24 hours. 10 g of the dried fruit powder was successively extracted with 100 ml of ethanol using Soxhlet apparatus and filtered through Whatman No 1 filter paper. The cytotoxic activity for cancer cell lines was evaluated by MTT assay. The in vitro cytotoxicity of different concentrations (18.75 - 300μg/mL) of the ethanolic extract of Cucumis melo fruit was evaluated by the MTT assay. The IC50 value is measured by the concentration of extract, causing 50% growth inhibition of cancer cells. The results indicated that the cytotoxic effect of the ethanolic extract of Cucumis melo fruit against HepG2 cells is dose-dependent. At low concentrations, the extract was found to be less toxic towards the HepG2 cells, whereas, at higher concentrations, the toxicity was increased. The concentration at 201.5 µg / ml was found to be an effective dose because, at this concentration, it exhibited 50 % cytotoxicity against HepG2 cells. This work revealed the potentials of ethanolic extract of Cucumis melo fruit as a cytotoxic agent against liver cancer cell lines. The plant can be further screened against various diseases using toxicity models in order to find out its unexplored efficacy.

scholarly journals The cytotoxic activity of pine needles ethanolic extract of Pinus merkusii on HeLa cell lines

2021 ◽  
Vol 33 ◽  
pp. 03001
Author(s):  
Annise Proboningrat ◽  
Amaq Fadholly ◽  
Sri Agus Sudjarwo ◽  
Fedik Abdul Rantam ◽  
Agung Budianto Achmad
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Anticancer Agent ◽  
Ethanolic Extract ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Lines ◽  
Cytotoxicity Assessment ◽  
Pinus Merkusii

Several efforts have been made to discover new anticancer agents based on natural ingredients. Meanwhile, previous studies have shown that different Pine genus species exhibit cytotoxic activity against various types of cancer cells. This plant is rich in phenolic compounds, especially procyanidins, flavonoids, and phenolic acids. Therefore, this study aims to investigate the in vitro cytotoxicity of Pinus merkusii needles extract on HeLa cancer cell lines. The cytotoxicity assessment was measured using MTT assay and expressed as IC50 value. The results showed that the ethanolic extract poses a dose and time-dependent cytotoxic activity with an IC50 value of 542.5 µg/ml at 48 hours of incubation. Based on this result, Pinus merkusii needles’ ethanolic extract has the potential of a novel candidate for an anticancer agent.

scholarly journals In-vitro antioxidant, anti-inflammatory and cytotoxic activity of Cucumis melo L. of ethanolic extract

Biomedicine ◽  
2021 ◽  
Vol 41 (2) ◽  
pp. 439-442
Author(s):  
G. Malathi ◽  
J. Vadivelu
Keyword(s):  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Cucumis Melo ◽  
Protein Denaturation ◽  
Ethanolic Extract ◽  
Cytotoxic Activities ◽  
Dpph Method ◽  
Anti Inflammatory ◽  
Cucumis Melo L

Introduction and Aim: Cucumis melo L. commonly known as wild melon (in English) belongs to the family Cucurbitaceae and well distributed in rural areas. Cucumis melo L. is a annual climbing or perennial herb, distributed almost throughout India and neighbouring countries.  Nowadays food wastes are a major concern. On the other hand, the demand for natural valuable compounds to human health is increasing. These by-products contain phytochemical compounds with great nutritional and functional potentials. The fruits of this plant possess the abortifacient property and have antitussive, antioxidant, digestive, cytotoxic and anti-inflammatory properties.   Materials and Methods: The ethanolic extract was used to determine the antioxidant scavenging activity using DPPH method, protein denaturation for anti inflammatory studies, as well as in vitro cytotoxic activity by brine shrimp lethal assay (BSLA).   Results: The results obtained indicated highest antioxidant activity as compared to DPPH methods. The protein denaturation test showed the ethanolic extracts of Cucumis melo L. had effective anti-inflammatory activity. Extracts of Cucumis melo L. also registered potential cytotoxic activities by BSLA. This study provides the basis for further investigation of Cucumis melo L. for potential identification of novel bioactive compounds with therapeutic properties.   Conclusion: The presence of phytoconstituents such as flavonoids, terpenoids, alkaloids, may indeed assist to the antioxidant activity of fruit extract. That this next thing that must be done will be to conduct in vivo experiments and discern the histopathological mechanism.

scholarly journals Antitumor Effect of the Essential Oil from the Leaves of Croton matourensis Aubl. (Euphorbiaceae)

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2974 ◽  
Author(s):  
Emilly Lima ◽  
Rafaela Alves ◽  
Gigliola D´Elia ◽  
Talita Anunciação ◽  
Valdenizia Silva ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Cancer Cell ◽  
Hepg2 Cells ◽  
Membrane Integrity ◽  
In Vitro Cytotoxicity ◽  
Cell Cycle Distribution ◽  
Antitumor Effects

Croton matourensis Aubl. (synonym Croton lanjouwensis Jabl.), popularly known as “orelha de burro”, “maravuvuia”, and/or “sangrad’água”, is a medicinal plant used in Brazilian folk medicine as a depurative and in the treatment of infections, fractures, and colds. In this work, we investigated the chemical composition and in vitro cytotoxic and in vivo antitumor effects of the essential oil (EO) from the leaves of C. matourensis collected from the Amazon rainforest. The EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized qualitatively and quantitatively by gas chromatography coupled to mass spectrometry (GC–MS) and gas chromatography with flame ionization detection (GC–FID), respectively. In vitro cytotoxicity of the EO was assessed in cancer cell lines (MCF-7, HCT116, HepG2, and HL-60) and the non-cancer cell line (MRC-5) using the Alamar blue assay. Furthermore, annexin V-FITC/PI staining and the cell cycle distribution were evaluated with EO-treated HepG2 cells by flow cytometry. In vivo efficacy of the EO (40 and 80 mg/kg/day) was demonstrated in C.B-17 severe combined immunodeficient (SCID) mice with HepG2 cell xenografts. The EO included β-caryophyllene, thunbergol, cembrene, p-cymene, and β-elemene as major constituents. The EO exhibited promising cytotoxicity and was able to cause phosphatidylserine externalization and DNA fragmentation without loss of the cell membrane integrity in HepG2 cells. In vivo tumor mass inhibition rates of the EO were 34.6% to 55.9%. Altogether, these data indicate the anticancer potential effect of C. matourensis.

scholarly journals The Synthesis, Characterization, Cytotoxic Activity Assessment and Structure–Activity Relationship of 4-Aryl-6-(2,5-dichlorothiophen-3-yl)-2-methoxypyridine-3-carbonitriles

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4072
Author(s):  
Al-Refai ◽  
Ibrahim ◽  
Azmi ◽  
Osman ◽  
Bakar ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Basic Medium ◽  
Condensation Process ◽  
Cytotoxicity Activities ◽  
Cytotoxicity Assessment ◽  
Relationship Of

A series of 2-methoxypyridine-3-carbonitrile (5a–i)-bearing aryl substituents were successfully synthesized in good yields by the condensation of chalcones (4a–i) with malononitrile in basic medium. The condensation process, in most cases, offers a route to a variety of methoxypyridine derivatives (6a–g) as side products in poor yields. All new compounds were fully characterized using different spectroscopic methods. Mass ESI-HMRS measurements were also performed. Furthermore, these compounds were screened for their in vitro cytotoxicity activities against three cancer cell lines; namely, those of the liver (line HepG2), prostate (line DU145) and breast (line MBA-MB-231). The cytotoxicity assessment revealed that compounds 5d, 5g, 5h and 5i exhibit promising antiproliferative effects (IC50 1–5 µM) against those three cancer cell lines.

Synthesis and Сytotoxicity of A-Azepanobetulinic Acid N-Methyl-Piperazinylamide

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986067 ◽  
Author(s):  
Gulnara V. Giniyatullina ◽  
Oxana B. Kazakova ◽  
Irina P. Baikova ◽  
Emil Yu. Yamansarov ◽  
Ilya A. Osterman ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antibacterial Agent ◽  
Cancer Cell Lines ◽  
Beckmann Rearrangement ◽  
Betulonic Acid

The synthesis of A-azepanobetulinic acid N-methylpiperazinylamide was performed through a series of transformations (oximation, Beckmann rearrangement, reduction) of previously synthesized betulonic acid N-methylpiperazinylamide. In vitro cytotoxic activity was detected for the obtained compound against a number of cancer cell lines, and its potential was revealed as an antibacterial agent.

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