Detection Of Measles Virus Rna For Genotyping By Rt-Pcr Assays Using Thermo Cycler

Objectives: An association between the measles virus and Hodgkin lymphoma has been disclosed by our laboratory in Beer-Sheva, starting in 2003. We question the refutation of our study and the absence of interest among experts. Methodology: It was based on immunohistochemistry with commercial, as well as experimental anti-measles antibodies. It relied also on RT-PCR and in situ hybridization evidence of measles virus RNA. Key Results: At this stage (2004), the link between the virus and the lymphoma was essentially descriptive. The first and last response to our challenge appeared in 2007, in the form of doublet articles, in the same issue of a major cancer journal. The two European research groups responding, rejected categorically our findings by proposing different arguments. Major Conclusion: As reservations to these reactions became soon apparent, a series of papers from our laboratory were published. These articles concerned the evidence of a relationship between the measles virus and additional categories of cancers. Different malignancies in which this virus was not expressed at all, were also described. A further study suggested a mechanism by which the measles virus may activate lymphomagenesis in classic Hodgkin lymphoma. To our dismay, and in spite of repeated calls to verify the various results, no further response was obtained from international experts.

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Persistent Measles Virus Infection and Otosclerosis

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348940111001001 ◽

2001 ◽

Vol 110 (10) ◽

pp. 897-903 ◽

Cited By ~ 22

Author(s):

Wolfgang Arnold ◽

Hans P. Niedermeyer ◽

Maria Schuster ◽

Wolfgang J. Neubert ◽

Christa Baumann ◽

...

Keyword(s):

Cell Culture ◽

Polymerase Chain Reaction ◽

Measles Virus ◽

Otic Capsule ◽

Rt Pcr ◽

Chain Reaction ◽

Virus Rna ◽

Polymerase Chain ◽

H Protein ◽

Antibody Pattern

The cause of otosclerosis is still unknown. Recently, measles virus involvement has been implicated. The aim of this study was to analyze the presence of measles virus RNA within the otosclerotic focus and to evaluate the perilymphatic antibody pattern. Bone and perilymph specimens from 40 patients with the spontaneous form of otosclerosis and from control patients were investigated by reverse transcription polymerase chain reaction (RT-PCR), Western blot techniques, and cell culture. By the use of RT-PCR, measles virus RNA could be detected in 32 patients, but not in controls. Analysis of perilymph revealed the presence of antibodies to N, F1, and M measles virus proteins in all cases, and antibodies against H protein in 2 additional cases. In preosteoblasts cultured from otosclerotic bone chips, no measles virus RNA could be amplified. We conclude that the spontaneous form of otosclerosis is, in the vast majority of cases, a measles virus-associated disease of the otic capsule.

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Estimation of two real-time RT-PCR assays for quantitation of hepatitis C virus RNA during PEG-IFN plus ribavirin therapy by HCV genotypes and IL28B genotype

Journal of Infection and Chemotherapy ◽

10.1007/s10156-012-0452-1 ◽

2013 ◽

Vol 19 (1) ◽

pp. 63-69 ◽

Cited By ~ 3

Author(s):

Yugo Miyagi ◽

Hideyuki Nomura ◽

Nobuyuki Yamashita ◽

Hironori Tanimoto ◽

Tsunefumi Shibuya ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Real Time ◽

Il28b Genotype ◽

Rt Pcr ◽

Hepatitis C Virus Rna ◽

Hcv Genotypes ◽

Virus Rna ◽

Pcr Assays

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Comparison of real-time RT-PCR assays for hepatitis E virus RNA detection

Journal of Clinical Virology ◽

10.1016/j.jcv.2013.06.038 ◽

2013 ◽

Vol 58 (1) ◽

pp. 36-40 ◽

Cited By ~ 34

Author(s):

Camelia Mokhtari ◽

Eric Marchadier ◽

Stephanie Haïm-Boukobza ◽

Asma Jeblaoui ◽

Sophie Tessé ◽

...

Keyword(s):

Real Time ◽

Hepatitis E Virus ◽

Hepatitis E ◽

Rt Pcr ◽

Rna Detection ◽

Virus Rna ◽

Pcr Assays

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Development of quantitative gene-specific real-time RT-PCR assays for the detection of measles virus in clinical specimens

Journal of Virological Methods ◽

10.1016/j.jviromet.2005.10.006 ◽

2006 ◽

Vol 132 (1-2) ◽

pp. 166-173 ◽

Cited By ~ 101

Author(s):

Kimberly B. Hummel ◽

Luis Lowe ◽

William J. Bellini ◽

Paul A. Rota

Keyword(s):

Real Time ◽

Measles Virus ◽

Rt Pcr ◽

Clinical Specimens ◽

Pcr Assays

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Absence of Measles Virus Detection from Stapes of Patients with Otosclerosis

Otolaryngology ◽

10.1177/0194599817733674 ◽

2017 ◽

Vol 158 (1) ◽

pp. 158-162 ◽

Cited By ~ 2

Author(s):

María de Lourdes Flores-García ◽

Claudia Adriana Colín-Castro ◽

Mario Sabas Hernández-Palestina ◽

Roberto Sánchez-Larios ◽

Rafael Franco-Cendejas

Keyword(s):

Real Time ◽

Measles Virus ◽

Genetic Material ◽

Virus Detection ◽

Cross Sectional Study ◽

Rt Pcr ◽

Tertiary Referral Hospital ◽

Cross Sectional ◽

Virus Rna ◽

N 93

Objective To determine molecularly the presence of measles virus genetic material in the stapes of patients with otosclerosis. Study Design A cross-sectional study. Setting A tertiary referral hospital. Subjects and Methods Genetic material was extracted from the stapes of patients with otosclerosis (n = 93) during the period from March 2011 to April 2012. The presence of viral measles sequences was evaluated by the real-time reverse transcriptase polymerase chain reaction (RT-PCR). The expression of the CD46 gene was determined. Results Ninety-three patients were included in the study. No sample was positive for any of 3 measles virus genes (H, N, and F). Measles virus RNA was not detected in any sample by real-time RT-PCR. CD46 levels were positive in 3.3% (n = 3) and negative in 96.7% (n = 90). Conclusion This study does not support the theory of measles virus as the cause of otosclerosis. It is necessary to do more research about other causal theories to clarify its etiology and prevention.

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Results of isolation and genotyping of measles viruses that circulated in 2012–2017 in the Odesa region

ACTUAL INFECTOLOGY ◽

10.22141/2312-413x.9.5-6.2021.246693 ◽

2021 ◽

Vol 9 (5-6) ◽

pp. 27-32

Author(s):

T.L. Hrydina ◽

V.O. Honcharov ◽

L.S. Kotlik ◽

O.V. Skopenko ◽

O.A. Hruzevsky ◽

...

Keyword(s):

Measles Virus ◽

State Institution ◽

The State ◽

Epidemic Outbreak ◽

Rt Pcr ◽

Regional Laboratory ◽

Virus Rna ◽

Different Strains ◽

Virus Strains ◽

Southern Ukraine

Background. The circulation of different strains of the measles virus is closely related to the region and the incidence rate since circulating strains can change during epidemic outbreaks and in interepidemic periods. According to the WHO, the B3 strain is most common during outbreaks worldwide. Therefore, typing of circulating strains of measles virus, especially during an epidemic outbreak, is an important process, including for predicting the development of an epidemic. The study was aimed to identify and determine the genotype of measles virus types that circulate in Ukraine during 2012–2019. Materials and methods. Materials of the reporting documentation of the State Institution “Odessa Regional Laboratory Center of the Ministry of Health of Ukraine” in the Odessa region during 2012–2019 were used and analyzed. Materials from patients with suspected measles were used for molecular biological, genetic, analytical, and statistical approaches investigation. Following the standard WHO protocol for sequencing and phylogenetic analysis, circulating measles virus strains were isolated from the patient in a special culture of Vero/SLAM cells. Measles virus RNA was isolated from the resulting virus-containing material after cultivation and RT-PCR was performed. The resulting cDNA was sent for genotyping, which was carried out at the WHO reference laboratory for the diagnosis of measles and rubella in Luxembourg (WHO RRL). Results. Twenty strains of measles virus from 45 samples (urine and nasopharyngeal swabs) from patients diagnosed with measles were isolated during 2012–2014. Virus isolation was not carried out in 2015–2016 due to isolated cases of the disease. Twenty-four virus strains from 164 samples were isolated in 2017. Conclusions. The results obtained at the State Institution “Odessa Regional Laboratory Center” demonstrated that during the interepidemic period of 2012–2014, the D4 genotype circulated in the region. But since 2017, when there was an increas of cases associated with a new epidemic outbreak, B3, genetic line MVs/Kabul.AFG/20.2014/3 B3 mainly circulates in the region of southern Ukraine. As you can see, these data completely coincide with the data about circulating genotypes that were found at a certain time in the European Region, according to the data from the literature.

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Comparison of monoplex and duplex RT-PCR assays for the detection of measles virus

Journal of Virological Methods ◽

10.1016/j.jviromet.2016.11.003 ◽

2017 ◽

Vol 239 ◽

pp. 58-60 ◽

Cited By ~ 1

Author(s):

Khalifa Binkhamis ◽

Hayley Gillis ◽

Joseph Daniel Lafreniere ◽

Joanne Hiebert ◽

Lillian Mendoza ◽

...

Keyword(s):

Measles Virus ◽

Rt Pcr ◽

Pcr Assays

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Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-021-04159-9 ◽

2021 ◽

Author(s):

Ute Eberle ◽

◽

Clara Wimmer ◽

Ingrid Huber ◽

Antonie Neubauer-Juric ◽

...

Keyword(s):

Real Time ◽

Rt Pcr ◽

Diagnostic Assays ◽

Molecular Assays ◽

Pcr Assays ◽

Laboratory Experiences

AbstractTo face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory.

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