scholarly journals Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan

2014 ◽  
Vol 3 (3) ◽  
pp. 173-177 ◽  
Author(s):  
Obaidullah Qazi ◽  
Hazir Rahman ◽  
Zarfishan Tahir ◽  
Muhammad Qasim ◽  
Sajid Khan ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rpob Gene ◽  
Multidrug Resistant ◽  
Rifampicin Resistance ◽  
Mutation Pattern

Emergence of Heteroresistance Mycobacterium Tuberculosis in Saudi Arabia

2020 ◽  
Vol 20 (4) ◽  
pp. 491-494 ◽  
Author(s):  
Eltayib H. Ahmed Abakur ◽  
Tarig M.S. Alnour ◽  
Faisel Abuduhier ◽  
Fahad M.A. Albalawi ◽  
Khalid A.S. Alfifi
Keyword(s):  
Saudi Arabia ◽  
Mycobacterium Tuberculosis ◽  
Clinical Specimen ◽  
Rpob Gene ◽  
Rifampicin Resistance ◽  
Resistant Bacteria ◽  
Kingdom Of Saudi Arabia ◽  
Preliminary Stage ◽  
The North ◽  
Genotype Mtbdrplus

Purpose: Heteroresistant Mycobacterium tuberculosis (MTB) is defined as a group of drug-susceptible and resistant bacteria in a single clinical specimen from tuberculosis (TB) patients. Heteroresistance of MTB is considered a preliminary stage to full resistance. The present study aimed to determine the heteroresistance in Mycobacterium tuberculosis in Tabuk province, in the north of the Kingdom of Saudi Arabia. Method: GenoType MTBDRplus assay was used to determine mutations associated with isoniazid and rifampicin resistance. Results: A total number of 46 confirmed M. tuberculosis positive sputum samples were scanned for heteroresistance. The present study revealed 3 (6.5%) heteroresistant mutations to either rpoB gene alone, 2 (4.4%) to rpoB and 1 (2.2%) to inhA genes. Conclusion: The detection of heteroresistant mutations could guide the initiation of an appropriate regimen of treatment.

scholarly journals Trends of Mycobacterium Tuberculosis and Rifampicin resistance in Adigrat General Hospital, Eastern zone, Tigray Region, Northern Ethiopia

2020 ◽  
Author(s):  
Getachew Kahsu Abay ◽  
Bahlibi Hailay
Keyword(s):  
Mycobacterium Tuberculosis ◽  
General Hospital ◽  
Data Extraction ◽  
Multidrug Resistant ◽  
Rifampicin Resistance ◽  
Northern Ethiopia ◽  
Drug Resistant ◽  
Cross Sectional ◽  
Eastern Zone ◽  
Previously Treated

Abstract Background: Tuberculosis is an infectious disease usually caused by Mycobacterium tuberculosis bacteria. The global emergence of mono- or multidrug-resistant tuberculosis and extensively drug-resistant forms of tuberculosis poses a considerable challenge to tuberculosis control programs. There has been no reliable and organized data on trends and drug resistance of Mycobacterium tuberculosis in the study area. Therefore, this study aimed to determine the trends of Mycobacterium tuberculosis and Rifampicin resistance in the Adigrat General Hospital, eastern zone, Tigray, Northern Ethiopia.Methods: A hospital-based retrospective cross-sectional study was conducted at Adigrat General Hospital from January 2015 to 2018. Data were collected retrospectively from the GenexpertTM TB registration books using a data extraction format. Data was entered into Epi-Info 3.1 and subsequently exported and analyzed using SPSS Version 20. The results were summarized using descriptive statistics, tables, and figures. P values < 0.05 were considered statistically significant.Result: A total of 5,944 Mycobacterium tuberculosis presumptive patients were included in the study. The majority of the study participants were male (58.1%) with participants’ median age of 40.0 (IQR 57, 26) years, the majority were 30-44 years. The overall positive cases of Mycobacterium tuberculosis was 24.3% (1446) with a total of 132 (9.1%) found to be resistant to rifampicin. Within the total Rifampicin resistant sub-group, 129/132 (97.7 %) were newly identified cases and the rest were previously treated, tuberculosis patients. Age, the reason for diagnosis, site of presumptive tuberculosis, and/or being HIV infected showed significant association with our dependent variable; however, only age and being HIV infected were associated with rifampicin resistance.Conclusion: In our study, the overall trends of Mycobacterium tuberculosis and rifampicin resistance were found to be high and gradually increasing. HIV co-infected and previously treated patients were more likely to develop rifampicin resistance. Therefore, maximizing early detection of drug-resistant Mycobacterium tuberculosis and strengthening tuberculosis infection control activities are recommended to reduce the burden of this contagious and potentially deadly disease.

scholarly journals Trends of Mycobacterium Tuberculosis and rifampicin Resistance in Adigrat General Hospital, Eastern zone of Tigrai, North Ethiopia

2020 ◽  
Author(s):  
Getachew Kahsu Abay ◽  
Bahlibi Hailay
Keyword(s):  
Mycobacterium Tuberculosis ◽  
General Hospital ◽  
Data Extraction ◽  
Multidrug Resistant ◽  
Cross Sectional Study ◽  
Rifampicin Resistance ◽  
Drug Resistant ◽  
Cross Sectional ◽  
Eastern Zone ◽  
Previously Treated

Abstract Background: Tuberculosis is an infectious disease usually caused by Mycobacterium tuberculosis bacteria. The global emergence of mono- or multidrug-resistant tuberculosis and extensively drug-resistant forms of tuberculosis poses a considerable challenge to tuberculosis control programs. There has been no reliable and organized data on trends and drug Resistance of Mycobacterium tuberculosis in the study area. Therefore, this study aimed to determine the trends of Mycobacterium tuberculosis and rifampicin Resistance in the Adigrat General Hospital, eastern zone of Tigrai, North Ethiopia.Methods: A hospital-based retrospective cross-sectional study was conducted at Adigrat General Hospital from January 2015 to 2018. Data were collected retrospectively from the GenexpertTM TB registration book using a data extraction format. Data was entered into Epi-Info 3.1 and subsequently exported and analyzed using SPSS Version 20. The results were summarized using descriptive statistics, tables, and figures. Bivariate and multi-variant regression analysis were employed to measure the association between dependent and independent variables. P values < 0.05 were considered statistically significant.Result: A total of 5,944 Mycobacterium tuberculosis presumptive patients were included in the study. The majority of the study participants were male (58.1%) with participants’ median age of 40.0 (IQR 26-57) years, the majority were 30-44 years. The overall positive cases of Mycobacterium tuberculosis was 24.3% (1446) with a total of 132 (9.1%) found to be resistant to Rifampicin. Within the total Rifampicin resistant sub-group, 129/1385 (9.3 %) were newly identified cases and the rest were previously treated, tuberculosis patients. Age, the reason for diagnosis, site of presumptive tuberculosis, and/or being HIV infected showed significant association with our dependent variable; however, only age and being HIV infected were associated with Rifampicin Resistance.Conclusion: In our study, the overall trends of Mycobacterium tuberculosis and rifampicin Resistance were found to be constant. HIV co-infected and previously treated patients were more likely to develop Rifampicin Resistance. Therefore, maximizing early detection of drug-resistant Mycobacterium tuberculosis and strengthening tuberculosis infection control activities are recommended to reduce the burden of this contagious and potentially deadly disease.

scholarly journals Epidemiology of Rifampicin Resistant Tuberculosis and Common Mutations in rpoB Gene of Mycobacterium tuberculosis: A Retrospective Study from Six Districts of Punjab (India) Using Xpert MTB/RIF Assay

2016 ◽  
Vol 8 (02) ◽  
pp. 096-100 ◽  
Author(s):  
Ramandeep Kaur ◽  
Neerja Jindal ◽  
Shilpa Arora ◽  
Shajla Kataria
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Surrogate Marker ◽  
Rpob Gene ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Resistance Mutations ◽  
Resistant Tuberculosis ◽  
Mdr Tb ◽  
Previously Treated Patients ◽  
User Friendly

ABSTRACT Background: Xpert MTB/RIF assay has revolutionized the diagnosis of tuberculosis (TB) by simultaneously detecting the bacteria and resistance to rifampicin (RIF), a surrogate marker for multidrug-resistant TB (MDR-TB) in <2 h. The RIF resistance pattern in Malwa region of Punjab, India, is not documented. Here, we report the epidemiology of RIF-resistant TB and mutations in rpoB gene of Mycobacterium tuberculosis (MTB). Materials and Methods: A total of 1612 specimens received between October 2013 and February 2015 were tested by Xpert MTB/RIF assay following manufacturer’s instructions. The results thus obtained were analyzed using SPSS version 20.0.0 (SPSS Inc., Chicago, IL, USA) statistical software. Result: RIF resistance was statistically higher in previously treated patients in comparison to the new patients (P = 0.006) and in patients with acid fast-Bacilli (AFB) positive smears to AFB-negative smears (P = 0.048). RIF resistance mutations in 130 specimens revealed frequency of E 73/130 (56%), B 28/130 (21.5%), D 18/130 (13.8%), A 11/130 (8.4%), and C 1/130 (0.7%) while in one specimen, mutation combination, i.e., mutations associated with more than one probe (A and B both) was present. Conclusion: Xpert MTB/RIF assay is a user-friendly screening tool for detection of MTB and RIF resistance from suspected TB/MDR cases in a shorter period of time. It could also serve as a useful technique to have simultaneous preliminary information regarding the mutation pattern of RIF resistance in MTB isolates.

scholarly journals Trends in Mycobacterium Tuberculosis and prevalence of Rifampicin Resistance in Eastern zone, Tigray Region, Northern Ethiopia

2020 ◽  
Author(s):  
Getachew Abay Kahsu ◽  
Bahlibi Hailay
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Data Extraction ◽  
Multidrug Resistant ◽  
Cross Sectional Study ◽  
Rifampicin Resistance ◽  
Northern Ethiopia ◽  
Drug Resistant ◽  
Cross Sectional ◽  
Resistant Tuberculosis ◽  
Eastern Zone

Abstract Background Tuberculosis (TB) is an infectious disease usually caused by Mycobacterium tuberculosis (MTB) bacteria. The emergence of Mono or multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis (XDR-TB), poses a considerable challenge to Mycobacterium tuberculosis control programs in the worldwide; however, there has been no reliable and organized data on trends and prevalence drug resistance of Mycobacterium tuberculosis in study area; Therefore, aim of this study to determine the trends of Mycobacterium tuberculosis and prevalence of Rifampicin resistance in eastern zone, Tigray, Northern Ethiopia. Methods Hospital based retrospective cross-sectional study was conducted at Adigrat General Hospital from June 01 to August 30, 2019.Data was collected retrospectively from the registration books using data extraction format commence January 01, 2015, December 30, 2018. Data was entered into Epi-Info 3.1 and exported and analyzed using SPSS Version 20. The results were summarized using descriptive statistics, tables, and figures. P values < 0.05 would be considered statistically significant. Result A total of 5,944 Mycobacterium tuberculosis presumptive patients were included in the study. The majority of the study participants were male (58.1%). The median age of the participants was 40.0 (IQR 57, 26) years, the majority age-group was 30-44 years. The overall prevalence of Mycobacterium tuberculosis was 1446 (24.3%). Of the total confirmed cases, 132 (9.1%) were resistant to rifampicin. From total Rifampicin resistant 129 (97.7%) new cases and the rest were previously treated tuberculosis patients. Age, reason for diagnosis, site of presumptive tuberculosis, being HIV infected was found a significant association with our dependent variable; however, only Age and being HIV infected associated with rifampicin resistance. Conclusion In our study, the overall trends of Mycobacterium tuberculosis and prevalence of rifampicin resistance were found high and increased; therefore, maximizing early detection of drug-resistant Mycobacterium tuberculosis and strengthening TB infection control activities and proper implementation of directly observed treatment are recommended reducing the burden of this contagious disease.

Sign in / Sign up

Export Citation Format

Share Document