High prevalence of multidrug-resistant tuberculosis among patients with rifampicin resistance using GeneXpert Mycobacterium tuberculosis/rifampicin in Ghana

Objective: Drug resistant tuberculosis has long been a common problem prevailing in developing countries including Bangladesh. Present study focused on the rapid identification of live Mycobacterium tuberculosis among treatment failure cases.Materials and Methods: Sputum samples from a total of 100 category-I and category-II treatment failure cases, assumed as multidrug resistant tuberculosis, were studied through fluorescein diacetate (FDA) staining under light emitting diode (LED) fluorescence microscope. Considering culture method as gold standard, we also compared the results of FDA staining with that of auramine O staining.Results: A total of 85% acid-fast bacilli were detected by FDA staining, 82% by auramine O staining and a total of 85% isolates were detected in Lowenstein-Jensen (LJ) culture. The sensitivity of FDA staining (96.47%) was estimated to be slightly higher than that of auramine O staining (91.76%). Moreover,76.47% cases were detected as multidrug resistant tuberculosis (MDR-TB). Conclusion: Taken together, FDA staining method has been proposed to be appropriate for the rapid diagnosis of drug resistant tuberculosis. DOI: http://dx.doi.org/10.3329/bjms.v11i4.12605 Bangladesh Journal of Medical Science Vol. 11 No. 04 Oct12

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Why Phenotypic Drug Susceptibility Testing of Mycobacterium tuberculosis to First-Line Drugs is not Sufficient for Proper Management of Drug-Resistant and Multidrug-Resistant tuberculosis?

Journal of Bacteriology & Parasitology ◽

10.4172/2155-9597.1000e128 ◽

2017 ◽

Vol 09 (01) ◽

Author(s):

Suhail Ahmad

Keyword(s):

Mycobacterium Tuberculosis ◽

Susceptibility Testing ◽

Drug Susceptibility ◽

Multidrug Resistant ◽

Drug Susceptibility Testing ◽

Drug Resistant ◽

First Line ◽

Proper Management ◽

Resistant Tuberculosis ◽

Multidrug Resistant Tuberculosis

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T cell cytokine responses in peripheral blood mononuclear cells from patients with multidrug-resistant tuberculosis following stimulation with proteins purified from Mycobacterium tuberculosis MDR clinical isolates

International Journal of Mycobacteriology ◽

10.1016/j.ijmyco.2016.10.009 ◽

2016 ◽

Vol 5 ◽

pp. S132-S133

Author(s):

Alireza Hadizadeh Tasbiti ◽

Shamsi Yari ◽

Mostafa Ghanei ◽

Seyed Davar Siadat ◽

Amir Amanzadeh ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Multidrug Resistant ◽

Peripheral Blood Mononuclear ◽

Resistant Tuberculosis ◽

Multidrug Resistant Tuberculosis ◽

Cytokine Responses ◽

Blood Mononuclear Cells

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Sulfamethoxazole Susceptibility of Mycobacterium tuberculosis Isolates from HIV-Infected Ugandan Adults with Tuberculosis Taking Trimethoprim-Sulfamethoxazole Prophylaxis

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01101-15 ◽

2015 ◽

Vol 59 (9) ◽

pp. 5844-5846 ◽

Cited By ~ 3

Author(s):

Sam Ogwang ◽

Caryn E. Good ◽

Brenda Okware ◽

Mary Nsereko ◽

Michael R. Jacobs ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Multidrug Resistant ◽

Sub Saharan Africa ◽

Content Type ◽

Pulmonary Tb ◽

Resistant Tuberculosis ◽

Multidrug Resistant Tuberculosis ◽

Sub Saharan

ABSTRACTAdditional drugs are needed for the treatment of multidrug-resistant tuberculosis (TB). Sulfamethoxazole has been shown to havein vitroactivity againstMycobacterium tuberculosis; however, there is concern about resistance given the widespread use of trimethoprim-sulfamethoxazole prophylaxis among HIV-infected patients in sub-Saharan Africa. Thirty-eight of 40Mycobacterium tuberculosisisolates (95%) from pretreatment sputum samples from Ugandan adults with pulmonary TB, including HIV-infected patients taking trimethoprim-sulfamethoxazole prophylaxis, were susceptible with MICs of ≤38.4 μg/ml.

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