Nanofibrous scaffolds of carboxymethyl guargum potentiated with reduced graphene oxide for wound healing applications

Non-healing chronic wounds are the key concern in type-2 diabetes that frequently leads to chronic infections, finally causing amputation of limbs, organs etc. Decrease in the proliferation and migration of cells such as keratinocytes and fibroblasts is the major reason for the development of such chronic diabetic wounds. Multiple evidences have shown that CTGF and reduced graphene oxide possesses angiogenic property and promote wound healing by promoting proliferation and migration of fibroblasts and keratinocytes cells.Conjugation of rGO with CTGF using EDC-NHS chemistry is a novel approach to accelerate the wound healing process. In the current work, we have developed a rGO/CTGF incorporated GelMA hydrogel dressing to improve wound healing by increasing proliferation and migration of cells as well as promoting formation of new blood vessels for increased supply of nutrients, oxygen and growth factors to wound area

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A Novel Functionalization of Bioactive Antimicrobial Peptide onto the Nano-CeO2/Reduced Graphene Oxide Cluster Type Biocomposite Wound Dressings for Diabetic Wound Care Management: In Vitro and In Vivo Evaluations

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2020.2222 ◽

2020 ◽

Vol 10 (1) ◽

pp. 37-45 ◽

Cited By ~ 1

Author(s):

Ran Wei ◽

Shijun Nie ◽

Jing Ma ◽

Changmei Feng ◽

Hongyu Kuang

Keyword(s):

Wound Healing ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Healing Process ◽

Wound Healing Process ◽

Diabetic Patients ◽

Diabetic Wound ◽

Reduced Graphene ◽

Diabetic Wound Healing

Diabetic wound ulcers and unhealed ulcerations have caused in severe difficulties of diabetic patients all around the world. The anti-microbial peptides (AMP) and nanomaterials have been demonstrated beneficial in diabetic wound healing studies. Diabetic wound healing process can be delayed significantly due to the infection issues. Hence in this report, biologically synthesized cerium oxide (CeO2) nanoparticles incorporated with reduced graphene oxide (RGO) and AMP for effective and rapid diabetic wound healing have been reported. At first, reduced graphene oxide was prepared from graphene oxide to prepare CeO2/RGO nanocomposites. Biocompatible AMP (IP-1) functionalized CeO2/RGO nanocomposites were accordingly prepared to study the diabetic wound healing process. Rats, the wound healing models with the wound size of about 1.5 cm2 were tested with the as-prepared samples. Further, the prepared samples were tested in a wound healing model of rate with the wound of size 1.5 cm2. Almost complete recovery of wounds i.e., 100% closure of wound area was observed after 2 weeks of treatment by the prepared samples. Biocompatible AMP (IP-1) functionalized CeO2/RGO nanocomposites enhances rapid keratinocytes proliferation by short time thereby it proves the enhanced ability of the prepared samples to act as a wound healing in-vivo drugs. HE and MTC staining protocols to illustrate histological observations elucidates the keratinocytes creation by biocompatible AMP (IP-1) functionalized CeO2/RGO nanocomposites.

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