Expression of costimulatory molecules (CD80, CD86, CD28, CD152), accessory molecules (TCR αβ, TCR γδ) and T cell lineage molecules (CD4+, CD8+) in PBMC of leprosy patients using Mycobacterium leprae antigen (MLCWA) with murabutide and T cell peptide of Trat protein

2004 ◽  
Vol 4 (1) ◽  
pp. 1-14 ◽  
Author(s):  
K. Sridevi ◽  
Khanna Neena ◽  
K.T. Chitralekha ◽  
A.K. Arif ◽  
D. Tomar ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lineage ◽  
Mycobacterium Leprae ◽  
Costimulatory Molecules ◽  
Leprosy Patients ◽  
Accessory Molecules

scholarly journals Lsr2 Peptides of Mycobacterium leprae Show Hierarchical Responses in Lymphoproliferative Assays, with Selective Recognition by Patients with Anergic Lepromatous Leprosy

2011 ◽  
Vol 80 (2) ◽  
pp. 742-752 ◽  
Author(s):  
Mehervani Chaduvula ◽  
A. Murtaza ◽  
Namita Misra ◽  
N. P. Shankar Narayan ◽  
V. Ramesh ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Mycobacterium Leprae ◽  
Lepromatous Leprosy ◽  
Healthy Controls ◽  
Content Type ◽  
Tuberculosis Patients ◽  
Cell Responses ◽  
Leprosy Patients

ABSTRACTLsr2 protein ofMycobacterium lepraewas shown earlier to elicit B and T cell responses in leprosy patients (20, 28). Lymphoproliferation toM. lepraeand Lsr2 antigens was observed in >70% of tuberculoid (T) patients and in 16 and 34% of lepromatous (L) patients, respectively. We focused on theM. lepraenonresponders in the lepromatous group using 22 synthetic Lsr2 peptides (end-to-end peptides A to F and overlapping peptides p1 to p16) inin vitroT cell responses. A total of 125 leprosy and 13 tuberculosis patients and 19 healthy controls from the area of endemicity (here, healthy controls, or HC) were investigated. The highest responses were observed (67 to 100%) in HC for all peptides except p1 to p3, and the lowest was observed in tuberculosis patients. Significant differences in lymphoproliferation were observed in T, L, and HC groups (analysis of variance [ANOVA],P= 0.000 to 0.015) for all end-to-end peptides except B and for p5 and p7 to p10. Hierarchical recognition between lepromatous and tuberculoid leprosy was noted for p8 (P< 0.05) and between the HC and L groups for p7 to p10, p15, and p16 (P< 0.005 toP< 0.02). Significant lymphoproliferation was observed to peptides A to F and p1 to p9, p11, p12, p15, p16 (P= 0.000 to 0.001) with 40% responding to peptides C and p16 in L patients. Lepromatous patients also showed significantly higher levels of a gamma interferon (IFN-γ) response to peptide C than to other peptides (P< 0.05). Major histocompatibility complex (MHC) class II bias for peptide recognition was not observed. These studies indicate that Lsr2 has multiple T cell epitopes that inducein vitroT cell responses in the highly infective lepromatous leprosy patients.

scholarly journals Presence of Human T-Cell Responses to the Mycobacterium leprae 45-Kilodalton Antigen Reflects Infection with or Exposure to M. leprae

2001 ◽  
Vol 8 (3) ◽  
pp. 604-611 ◽  
Author(s):  
Anne Macfarlane ◽  
Rafael Mondragon-Gonzalez ◽  
Francisco Vega-Lopez ◽  
Brigitte Wieles ◽  
Josefina de Pena ◽  
...  
Keyword(s):  
T Cell ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Leprae ◽  
The Other ◽  
Recombinant Antigens ◽  
Diagnostic Potential ◽  
Human T Cell ◽  
Tuberculoid Leprosy ◽  
Leprosy Patients ◽  
Ifn Γ

ABSTRACT The ability of the 45-kDa serine-rich Mycobacterium leprae antigen to stimulate peripheral blood mononuclear cell (PBMC) proliferation and gamma interferon (IFN-γ) production was measured in leprosy patients, household contacts, and healthy controls from areas of endemicity in Mexico. Almost all the tuberculoid leprosy patients gave strong PBMC proliferation responses to the M. leprae 45-kDa antigen (92.8%; n = 14). Responses were lower in lepromatous leprosy patients (60.6%;n = 34), but some responses to the 45-kDa antigen were detected in patients unresponsive to M. lepraesonicate. The proportion of positive responses to the M. leprae 45-kDa antigen was much higher in leprosy contacts (88%; n = 17) than in controls from areas of endemicity (10%; n = 20). None of 15 patients with pulmonary tuberculosis gave a positive proliferation response to the 45-kDa antigen. The 45-kDa antigen induced IFN-γ secretion similar to that induced by the native Mycobacterium tuberculosis30/31-kDa antigen in tuberculoid leprosy patients and higher responses than those induced by the other recombinant antigens (M. leprae 10- and 65-kDa antigens, thioredoxin, and thioredoxin reductase); in patients with pulmonary tuberculosis it induced lower IFN-γ secretion than the other recombinant antigens. These results suggest that the M. leprae 45-kDa antigen is a potent T-cell antigen which is M. leprae specific in these Mexican donors. This antigen may therefore have diagnostic potential as a new skin test reagent or as an antigen in a simple whole-blood cytokine test.

scholarly journals Mimicking B and T cell epitopes between Mycobacterium leprae and host as predictive biomarkers in type 1 reaction in leprosy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vinay Kumar Pathak ◽  
Itu Singh ◽  
Shoor Vir Singh ◽  
Utpal Sengupta
Keyword(s):  
T Cell ◽  
Immune Responses ◽  
Basic Protein ◽  
Predictive Biomarkers ◽  
Mycobacterium Leprae ◽  
Autoimmune Response ◽  
T Cell Epitopes ◽  
Leprosy Patients

AbstractSeveral Mycobacterial infections including leprosy and tuberculosis are known to evoke autoimmune responses by modulating homeostatic mechanism of the host. Presence of autoantibodies like, rheumatoid factor, anti-nuclear factor and antibodies to host, collagen, keratin, myelin basic protein (MBP) and myosin, have been earlier reported in leprosy patients. In the present study, we detected the role of mimicking epitopes between Mycobacterium leprae and host components in the induction of autoimmune response in leprosy. Based on our previous findings, we predicted and synthesized a total of 15 mimicking linear B cell epitopes (BCE) and 9 mimicking linear T cell epitopes (TCE) of keratin and MBP. Humoral and cell-mediated immune responses against these epitopes were investigated in Non-reaction (NR), Type 1 reaction (T1R) leprosy patients, and healthy controls. We observed significantly higher levels of antibodies against 8 BCE in T1R in comparison to NR leprosy patients. Further, we also found 5 TCE significantly associated with lymphocyte proliferation in the T1R group. Our results indicated that these epitopes play a key role in the induction of autoimmune response in leprosy and are also strongly associated with the inflammatory episodes of T1R. Conclusively, these molecules may be employed as a biomarker to predict the inflammatory episodes of T1R.

T-cell recognition of peptides from the Mycobacterium leprae 35 kDa protein in Thai leprosy patients, healthy contacts, and non-contacts

2003 ◽  
Vol 88 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Boosbun Chua-Intra ◽  
Sukanya Wattanapokayakit ◽  
Sopa Srisungngam ◽  
Tawee Srisungngam ◽  
Krisada Mahotarn ◽  
...  
Keyword(s):  
T Cell ◽  
Mycobacterium Leprae ◽  
Cell Recognition ◽  
T Cell Recognition ◽  
Leprosy Patients ◽  
35 Kda Protein

scholarly journals Identification and Characterization of the ESAT-6 Homologue of Mycobacterium leprae and T-Cell Cross-Reactivity with Mycobacterium tuberculosis

2002 ◽  
Vol 70 (5) ◽  
pp. 2544-2548 ◽  
Author(s):  
Annemieke Geluk ◽  
Krista E. van Meijgaarden ◽  
Kees L. M. C. Franken ◽  
Yanri W. Subronto ◽  
Brigitte Wieles ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Mycobacterium Leprae ◽  
Cross Reactivity ◽  
Healthy Individuals ◽  
Mycobacterial Species ◽  
Cell Responses ◽  
Leprosy Patients ◽  
Identification And Characterization

ABSTRACT In this paper we describe identification and characterization of Mycobacterium leprae ESAT-6 (L-ESAT-6), the homologue of M. tuberculosis ESAT-6 (T-ESAT-6). T-ESAT-6 is expressed by all pathogenic strains belonging to the M. tuberculosis complex but is absent from virtually all other mycobacterial species, and it is a promising antigen for immunodiagnosis of tuberculosis (TB). Therefore, we analyzed whether L-ESAT-6 is a similarly powerful tool for the study of leprosy by examining T-cell responses against L-ESAT-6 in leprosy patients, TB patients, and exposed or nonexposed healthy controls from areas where leprosy and TB are endemic and areas where they are not endemic. L-ESAT-6 was recognized by T cells from leprosy patients, TB patients, individuals who had contact with TB patients, and healthy individuals from an area where TB and leprosy are endemic but not by T cells from individuals who were not exposed to M. tuberculosis and M. leprae. Moreover, leprosy patients who were not responsive to M. leprae failed to respond to L-ESAT-6. A very similar pattern was obtained with T-ESAT-6. These results show that L-ESAT-6 is a potent M. leprae antigen that stimulates T-cell-dependent gamma interferon production in a large proportion of individuals exposed to M. leprae. Moreover, our results suggest that there is significant cross-reactivity between T-ESAT-6 and L-ESAT-6, which has implications for the use of ESAT-6 as tool for diagnosis of leprosy and TB in areas where both diseases are endemic.

Mycobacterium Leprae-reactive T-cell clones isolated from polar lepromatous and tuberculoid leprosy patients

1987 ◽  
Vol 138 (5) ◽  
pp. 723-735 ◽  
Author(s):  
P. Launois ◽  
P. Shankar ◽  
D. Wallach ◽  
B. Flageul ◽  
F. Cottenot ◽  
...  
Keyword(s):  
T Cell ◽  
Mycobacterium Leprae ◽  
T Cell Clones ◽  
Cell Clones ◽  
Tuberculoid Leprosy ◽  
Leprosy Patients
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