Dihydrofisetin exerts its anti-inflammatory effects associated with suppressing ERK/p38 MAPK and Heme Oxygenase-1 activation in lipopolysaccharide-stimulated RAW 264.7 macrophages and carrageenan-induced mice paw edema

Lasia spinosa (L.) Thwaites was used as a traditional medicine to treat many inflammatory diseases for centuries. However, its effects on the inflammatory response are not yet characterized. In this study, we investigated the anti-inflammatory activities of L. spinosa leaf extract in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. We found that ethanol extracts of L. spinosa leaves showed anti-oxidant activity due to the presence of high levels of polyphenolic compounds. Treatment with the leaf extract significantly repressed the production of inflammatory mediators such as nitric oxide and reactive oxygen species and the expression of pro-inflammatory cytokines in the LPS-stimulated RAW 264.7 cells. Moreover, L. spinosa leaf extract treatment prevented activation of the nuclear factor-kappa B pathway by inhibiting nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) degradation. Furthermore, the mitogen-activated kinase and phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) pathways were suppressed upon treatment with the leaf extract. In addition to suppressing inflammatory factors, the extract also activated the nuclear factor erythroid 2-related factor 2/heme-oxygenase-1 pathway. We propose that L. spinosa leaf extract has the potential as an effective therapeutic agent for alleviating oxidative stress and excessive inflammation.

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Shepherd’s Purse Polyphenols Exert Its Anti-Inflammatory and Antioxidative Effects Associated with Suppressing MAPK and NF-κB Pathways and Heme Oxygenase-1 Activation

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/7202695 ◽

2019 ◽

Vol 2019 ◽

pp. 1-14 ◽

Cited By ~ 5

Author(s):

Jinming Peng ◽

Tianyong Hu ◽

Jin Li ◽

Jing Du ◽

Kerui Zhu ◽

...

Keyword(s):

Heme Oxygenase ◽

Chemical Constituents ◽

Chemical Compounds ◽

Heme Oxygenase 1 ◽

Mice Model ◽

Traditional Herbal Medicine ◽

Raw 264.7 Macrophages ◽

Antioxidative Effects ◽

Anti Inflammatory

Shepherd’s purse (Capsella bursa-pastoris (L.) Medik.), a wild herb as a traditional herbal medicine, has been proved with multiple healthy benefits. In this study, the chemical constituents of shepherd’s purse were identified by UPLC-QTOF-MS/MS. The antioxidative and anti-inflammatory potential of shepherd’s purse extract (SPE) were also investigated applying lipopolysaccharide- (LPS-) induced inflammation in RAW 264.7 macrophages and a carrageenan-induced mice paw edema model. Twenty-four chemical compounds were identified mainly including phenolic acids and flavonoids. The data also indicated SPE inhibited the productions of NO, PGE2, TNF-α, and IL-6 stimulated with LPS. In addition, SPE inhibited the increase of reactive oxygen species (ROS) and upregulated the expression of heme oxygenase-1 (HO-1). We further found that SPE inhibited the phosphorylation of P38 MAPK and activation of NF-κB. In vivo mice model also indicated that SPE showed strong antioxidative and anti-inflammatory activity.

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Anti-inflammatory effect of transduced PEP-1-heme oxygenase-1 in Raw 264.7 cells and a mouse edema model

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2011.06.147 ◽

2011 ◽

Vol 411 (2) ◽

pp. 354-359 ◽

Cited By ~ 14

Author(s):

Soon Won Kwon ◽

Eun Jeong Sohn ◽

Dae Won Kim ◽

Hoon Jae Jeong ◽

Mi Jin Kim ◽

...

Keyword(s):

Heme Oxygenase ◽

Raw 264.7 Cells ◽

Heme Oxygenase 1 ◽

Raw 264.7 ◽

Edema Model ◽

Anti Inflammatory ◽

Inflammatory Effect

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Involvement of Heme Oxygenase-1 Participates in Anti-Inflammatory and Analgesic Effects of Aqueous Extract ofHibiscus taiwanensis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/132859 ◽

2012 ◽

Vol 2012 ◽

pp. 1-13 ◽

Cited By ~ 8

Author(s):

Shu-Ling Liu ◽

Jeng-Shyan Deng ◽

Chuan-Sung Chiu ◽

Wen-Chi Hou ◽

Shyh-Shyun Huang ◽

...

Keyword(s):

Nitric Oxide ◽

Western Blotting ◽

Heme Oxygenase ◽

Aqueous Extract ◽

Heme Oxygenase 1 ◽

Natural Health Products ◽

Paw Edema ◽

Raw264.7 Macrophages ◽

Cox 2 ◽

Anti Inflammatory

Anti-inflammatory effects of the aqueous extract ofHibiscus taiwanensis(AHT) were used in lipopolysaccharide (LPS-)stimulated mouse macrophage RAW264.7 cells and carrageenan (Carr-)induced mouse paw edema model. When RAW264.7 macrophages were treated with AHT together with LPS, a concentration-dependent inhibition of nitric oxide (NO), tumor necrosis factor (TNF-α), and prostaglandin E2 (PGE2) levels productions were detected. Western blotting revealed that AHT blocked protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and elevated heme oxygenase-1 (HO-1), significantly. In the animal test, AHT decreased the paw edema at the 4th and the 5th h after Carr administration, and it increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the paw tissue. We also demonstrated AHT decreased the NO, TNF-α, and PGE2 levels on the serum level at the 5th h after the Carr injection. Western blotting revealed that AHT decreased Carr-induced iNOS, and COX-2, and increased HO-1 expressions at the 5th h in the edema paw. These findings demonstrated that AHT has excellent anti-inflammatory activitiesin vitroandin vivoand thus it has great potential to be used as a source for natural health products.

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