Chlamydia pneumoniae enhances CD4+ and CD8 +T effector memory cell IL-2 and IL-4 responses in stimulated peripheral blood mononuclear cells in human subjects

Abstract Zn is an essential nutrient for humans; however, a sensitive biomarker to assess Zn status has not been identified. The objective of this study was to determine the reliability and sensitivity of Zn transporter and metallothionein (MT) genes in peripheral blood mononuclear cells (PBMCs) to Zn exposure ex vivo and to habitual Zn intake in human subjects. In study 1, human PBMCs were cultured for 24 h with 0–50 µm ZnSO4 with or without 5 µm N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), and mRNA expression of SLC30A1-10, SLC39A1-14, MT1 subtypes (A, B, E, F, G, H, L, M and X), MT2A, MT3 and MT4 mRNA was determined. In study 2, fifty-four healthy male and female volunteers (31·9 (sd 13·8) years, BMI 25·7 (sd 2·9) kg/m2) completed a FFQ, blood was collected, PBMCs were isolated and mRNA expression of selected Zn transporters and MT isoforms was determined. Study 1: MT1E, MT1F, MT1G, MT1H, MT1L, MT1M, MT1X, MT2A and SLC30A1 increased with increasing concentrations of Zn and declined with the addition of TPEN. Study 2: Average daily Zn intake was 16·0 (sd 5·3) mg/d (range: 9–31 mg/d), and plasma Zn concentrations were 15·5 (SD 2·8) μmol/l (range 11–23 μmol/l). PBMC MT2A was positively correlated with dietary Zn intake (r 0·306, P = 0·03) and total Zn intake (r 0·382, P < 0·01), whereas plasma Zn was not (P > 0·05 for both). Findings suggest that MT2A mRNA in PBMCs reflects dietary Zn intake in healthy adults and may be a component in determining Zn status.

Download Full-text

Prevalence of Chlamydia pneumoniae in peripheral blood mononuclear cells in Italian patients with acute ischaemic heart disease

Atherosclerosis ◽

10.1016/s0021-9150(01)00537-8 ◽

2001 ◽

Vol 159 (2) ◽

pp. 521-525 ◽

Cited By ~ 44

Author(s):

Rosa Sessa ◽

Marisa Di Pietro ◽

Giovanna Schiavoni ◽

Iolanda Santino ◽

Paola Cipriani ◽

...

Keyword(s):

Heart Disease ◽

Ischaemic Heart Disease ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Chlamydia Pneumoniae ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

Download Full-text

Chlamydia pneumoniae in Peripheral Blood Mononuclear Cells Isolated from Individuals Younger than 20 Years or Older than 60 Years

Journal of Clinical Microbiology ◽

10.1128/jcm.43.6.3030.2005 ◽

2005 ◽

Vol 43 (6) ◽

pp. 3030-3030 ◽

Cited By ~ 7

Author(s):

S. J. Kohlhepp ◽

J. Hardick ◽

C. Gaydos

Keyword(s):

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Chlamydia Pneumoniae ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

Download Full-text

2221. Chlamydia pneumoniae (Cpn) Induces IFN-γ Responses in Peripheral Blood Mononuclear Cells (PBMC) from Pediatric and Adult Asthma Patients: Effects of Age and Inhaled Corticosteroid Use

Open Forum Infectious Diseases ◽

10.1093/ofid/ofz360.1899 ◽

2019 ◽

Vol 6 (Supplement_2) ◽

pp. S758-S758

Author(s):

Aviva Szigeti ◽

Margaret Hammerschlag ◽

Diana Weaver ◽

Tamar Smith-Norowitz ◽

Stephan Kohlhoff

Keyword(s):

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Chlamydia Pneumoniae ◽

Mononuclear Cells ◽

Inhaled Corticosteroid ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Asthma Patients ◽

Ifn Γ

Abstract Background Chlamydia pneumoniae (Cpn) is unique in its ability to cause chronic infections, potentially triggering asthma exacerbations as well as subsequent asthma development. Th1-mediated immunity and IFN-γ are critical for clearing chlamydial infections. Persistent or recent Cpn infection may be identified in vitro by detecting T-helper cytokine IFN-γ produced by peripheral blood mononuclear cells (PBMC) stimulated by Cpn. Inhaled corticosteroids (ICS) may have an inhibitory effect on IFN-γ. Prior studies have shown increased Th2 responses upon in vitro Cpn stimulation with increased age. Our aim was to determine whether age and inhaled corticosteroid (ICS) use affect Cpn-induced PBMC produced IFN-γ levels. Methods Pediatric and adult subjects with (n = 23) and without (n = 10) asthma were enrolled. PBMC obtained from all subjects were stimulated with Cpn (MOI = 0.1 x48h) in vitro. IFN-γ levels in culture supernatants were determined by ELISA and reported as pg/mL. Nasopharyngeal (NP) swabs were tested for Cpn using Real-Time PCR. Statistical analysis for continuous variables was performed using the Mann–Whitney U test. Results None of the subjects were positive for Cpn by PCR on NP swab. Levels of IFN-γ produced by PBMC stimulated by Cpn were similar between asthmatic vs. control subjects (41.7 vs. 68.8, respectively; P = 0.72) and between pediatric and adult subjects with asthma (IFN-γ 54 vs. 20.1 respectively, P = 0.95). Pediatric subjects with asthma who received ICS had lower IFN-γ levels than those who did not (median IFN-γ 25.5 vs. 209; P = 0.003). Conclusion Our finding of lower IFN-γ levels among asthma patients on ICS compared with those not on ICS suggests that ICS use may dampen the systemic inflammatory response. While we did not find a statistically significant difference between pediatric and adult age groups in this pilot study, there was a trend to higher Cpn-induced IFN-γ levels among younger pediatric subjects. Future prospective studies should further define predictors of diminished IFN-γ responses in patients with asthma. Disclosures All authors: No reported disclosures.

Download Full-text