P2-020: MicroRNA complexity in Alzheimer's disease cerebrospinal fluid, extracellular fluid and brain tissue biopsy

Determinations of acid monoamine metabolites, such as homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), in cerebrospinal fluid (CSF) give valid information on the metabolism of the corresponding amines in the brain tissue (Moir et al., 1970; Roos, 1970). The monoamine metabolites in the CSF are related to age. The concentrations of HVA and 5-HIAA increase with age (Gottfries et al., 1971). Probenecid blocks the elimination of HVA and 5-HIAA from brain tissue to blood (Neff et al., 1964, 1967; Werdinius, 1966) and from CSF to blood (Guldberg et al., 1966; Olsson and Roos, 1968). Probenecid thus normally induces an increase in the concentrations of the acid monoamine metabolites in the CSF, which is related to the turnover of monoamines in the brain tissue.

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Analysis of Brain Tissue and Cerebrospinal Fluid Feature for Alzheimer’s Disease Detection

2018 International Conference on Computer Engineering, Network and Intelligent Multimedia (CENIM) ◽

10.1109/cenim.2018.8711088 ◽

2018 ◽

Author(s):

Cucun Very Angkoso ◽

I Ketut Eddy Purnama ◽

Mauridhi Hery Purnomo

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Cerebrospinal Fluid ◽

Brain Tissue ◽

Disease Detection

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Citrulline supplementation improves spatial memory in a murine model for Alzheimer’s disease

10.1101/2020.06.15.153346 ◽

2020 ◽

Author(s):

Katia Martínez-González ◽

Leonor Serrano-Cuevas ◽

Eduardo Almeida-Gutiérrez ◽

Salvador Flores-Chavez ◽

Juan Manuel Mejía-Aranguré ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Cerebrospinal Fluid ◽

Brain Tissue ◽

Murine Model ◽

Ultra Performance Liquid Chromatography ◽

Oral Supplementation ◽

Arginine Deficiency ◽

Water Supplementation ◽

Viable Treatment

BackgroundAlzheimer’s disease (AD) correlates with the dysfunction of metabolic pathways that translates into neurological symptoms. An arginine deficiency, a precursor of nitric oxide (NO), has been reported for AD patients.ObjectiveWe aimed to evaluate the effect of citrulline oral supplementation on cognitive decline in an AD murine model.MethosThree-month citrulline or water supplementation was blindly given to male and female wild type and 3xTg-AD mice trained and tested in the Morris Water Maze (MWM). Cerebrospinal fluid and brain tissue were collected. Ultra Performance Liquid Chromatography was used for arginine determinations while the Griess method was used for NO.ResultsEight-month-old male 3xTg-AD mice supplemented with citrulline performed significantly better in the MWM task; arginine levels increased in cerebrospinal fluid although no changes were seen in brain tissue and only a tendency of increase of NO was observed.ConclusionsCitrulline oral administration is a viable treatment for memory improvement in the early stages of AD pointing to NO as a viable, efficient target for memory dysfunction in AD.

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