scholarly journals Possible synergy effect of hydrogen sulfide and acetate produced by sulfate-reducing bacteria on inflammatory bowel disease development

2021 ◽  
Vol 27 ◽  
pp. 71-78 ◽  
Author(s):  
Ivan Kushkevych ◽  
Dani Dordević ◽  
Monika Vítězová
Keyword(s):  
Inflammatory Bowel Disease ◽  
Hydrogen Sulfide ◽  
Bowel Disease ◽  
Sulfate Reducing Bacteria ◽  
Disease Development ◽  
Synergy Effect ◽  
Sulfate Reducing ◽  
Inflammatory Bowel ◽  
Reducing Bacteria

scholarly journals Possible methods for evaluation of hydrogen sulfide toxicity against lactic acid bacteria

2019 ◽  
Vol 9 (4) ◽  
pp. 4066-4069 ◽  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Hydrogen Sulfide ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bowel Disease ◽  
Inhibitory Concentration ◽  
Agar Plate ◽  
Intestinal Microbiome ◽  
Sulfate Reducing ◽  
Inflammatory Bowel

An integral part of the intestinal microbiota is undoubtedly formed by lactic acid bacteria (LAB). Their presence in the digestive tract is essential for its proper functioning. During inflammatory bowel disease, such as ulcerative colitis (UC), LAB occurrence is reduced while sulfate-reducing bacteria (SRB) occur widely in the intestine resulting in an increase of their metabolite, hydrogen sulfide. Inhibitory concentration and mechanism of action of hydrogen sulfide on LAB are not fully known yet. The aim of this paper is to find the proper testing methods for evaluation of the interaction between hydrogen sulfide and lactic acid bacteria, including minimal inhibitory concentration (MIC) determination. Spectrophotometric, colorimetric, agar plate methods and combination of these methods were tested. Thousands of people from all over the world are affected by inflammatory bowel disease every year. The gained results could help to understand and improve the stability of intestinal microbiome, improve the treatment of bowel inflammation diseases, or prevent bowel disease altogether

scholarly journals Analysis of pH dose-dependent growth of sulfate-reducing bacteria

Open Medicine ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Ivan Kushkevych ◽  
Dani Dordević ◽  
Monika Vítězová
Keyword(s):  
Ulcerative Colitis ◽  
Hydrogen Sulfide ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Alkaline Media ◽  
Dissimilatory Sulfate Reduction ◽  
Lactate Oxidation ◽  
Sulfate Reducing ◽  
Inflammatory Bowel ◽  
Reducing Bacteria

AbstractLower intraluminal colonic pH is an indication for the development of inflammatory bowel disease including active ulcerative colitis. Involvement of intestinal sulfate-reducing bacteria in decreasing bowel pH by the production of H2S and acetate as well as their sensitivity has never been reported before. The study of the relative pH and survival of Desulfovibrio piger Vib-7 by monitoring sulfate reduction parameters was the aim of this work. Monitoring was done through the measurement of bacterial growth (biomass), dissimilatory sulfate reduction parameters: sulfate consumption, lactate oxidation, hydrogen sulfide and acetate production. According to our results, we observed that lower pH (<5) significantly inhibited D. piger Vib-7 growth. This inhibition was also noticed when alkaline media (>9 pH) was used, though the reduction was not at the rate as in media with pH of 4. The research indicates that the growth of D. piger Vib-7 is inhibited at pH of 4 which is not as low as the pH found in people with severely developed inflammatory bowel diseases such as ulcerative colitis. Certainly the interaction (synergistic effect) between both hydrogen sulfide and acetate accumulation can also play an important etiological role in the development of bowel inflammation in humans and animals.

scholarly journals Molecular Cloning cysK Gene from Escherichia coli Genome, Transferring in the Intestinal Sulfate-Reducing Bacteria and the Expression Analysis of O-acetylserine(thiol)lyase

2016 ◽  
Vol 4 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Ivan V Kushkevych
Keyword(s):  
Escherichia Coli ◽  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Sulfate Reducing Bacteria ◽  
Wild Type ◽  
Mutant Type ◽  
Sulfate Reducing ◽  
Cysteine Synthesis ◽  
Inflammatory Bowel ◽  
Reducing Bacteria

Sulfate-reducing bacteria produce hydrogen sulfide which is toxic and carcinogenic for intestinal epithelial cells and can cause the development of the inflammatory bowel disease and ulcerative colitis in the humans and animals. Enzyme O-acetylserine(thiol)lyase, localized in Escherichia coli genome, use sulfide as substrate in the cysteine synthesis pathway. In this paper, the molecular cloning cysK gene from E. coli, its genetic transferring in the intestinal sulfate-reducing bacterium Desulfovibrio piger Vib-7 and the expression analysis of the enzyme was studied. Cysteine synthesis from hydrogen sulfide as substrate in the D. piger Vib-7 strain at the first time was demonstrated and characterized. The bacterial growth, sulfate and lactate consumption, accumulation of sulfide, acetate and cysteine synthesis in both D. piger Vib-7 wild-type and mutant-type were tested. The mutant-strain consumed much faster sulfate and lactate producing cysteine in the cultivation medium. The expression of the cysK gene in the mutant-type was studied by the formation of the final reaction product (cysteine) and the activity of O-acetylserine(thiol)lyase enzyme. Cysteine level was directly proportional to consumption of sulfate in the mutant-type and accumulation of sulfide in the wild-type. The D. piger Vib-7 mutant-type completely used sulfate the 48th hour of cultivation, thereafter additional sulfite and sulfide doses from the medium were also consumed and converted to cysteine. The obtained genetically constructed mutant strain bacterium D. piger Vib-7 for therapeutic strategy could be applied as a probiotic substance for subjects with inflammatory bowel disease and ulcerative colitis. This strain can compete with other intestinal sulfate-reducing bacteria, actively growth consuming sulfate and lactate much faster, and converting the toxic sulfide to untoxic cysteine in the gut.Microbes and Health, January 2015. 4(1): 19-24

Fluorescence chemosensors for hydrogen sulfide detection in biological systems

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1772-1786 ◽  
Author(s):  
Zhi Guo ◽  
Guiqiu Chen ◽  
Guangming Zeng ◽  
Zhongwu Li ◽  
Anwei Chen ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Biological Systems ◽  
Sulfate Reducing Bacteria ◽  
Fluorescence Sensing ◽  
Sulfate Reducing ◽  
Potential Applications ◽  
Reducing Bacteria

The development of H2S fluorescence-sensing strategies and their potential applications in the determination of sulfate-reducing bacteria activity.

Current recommendations on the role of diet in the aetiology and management of IBD

2021 ◽  
pp. flgastro-2020-101429
Author(s):  
Konstantinos Gerasimidis ◽  
Lihi Godny ◽  
Rotem Sigall-Boneh ◽  
Vaios Svolos ◽  
Catherine Wall ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Disease Onset ◽  
Well Being ◽  
Disease Development ◽  
Dietary Recommendations ◽  
Exclusive Enteral Nutrition ◽  
Established Disease ◽  
Inflammatory Bowel

Diet is a key modifier of risk of inflammatory bowel disease development and potentially a treatment option in patients with established disease. International organisations in gastroenterology and inflammatory bowel disease have published guidelines for the role of diet in disease onset and its management. Here, we discuss the major overarching themes arising from these guidelines and appraise recent literature on the role of diet for inflammatory bowel disease prevention, treatment of active disease and maintenance of remission, considering these themes. Except for exclusive enteral nutrition in active Crohn’s disease, we currently possess very little evidence to make any further dietary recommendations for the management of inflammatory bowel disease. There is also currently uncertainty on the extrapolation of epidemiological dietary signals on risk of disease development and preclinical experiments in animal models to management, once disease is established. Until high-quality evidence from clinical research becomes available, the only specific recommendations for inflammatory bowel disease we might safely give are those of healthy eating which apply for the general population for overall health and well-being.

Importance of TLR9-IL23-IL17 axis in inflammatory bowel disease development: Gene expression profiling study

2018 ◽  
Vol 197 ◽  
pp. 86-95 ◽  
Author(s):  
Sanja Dragasevic ◽  
Biljana Stankovic ◽  
Aleksandra Sokic-Milutinovic ◽  
Tomica Milosavljevic ◽  
Tamara Milovanovic ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Bowel Disease ◽  
Gene Expression Profiling ◽  
Bowel Disease ◽  
Expression Profiling ◽  
Disease Development ◽  
Inflammatory Bowel
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