Fluorescence chemosensors for hydrogen sulfide detection in biological systems

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1772-1786 ◽  
Author(s):  
Zhi Guo ◽  
Guiqiu Chen ◽  
Guangming Zeng ◽  
Zhongwu Li ◽  
Anwei Chen ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Biological Systems ◽  
Sulfate Reducing Bacteria ◽  
Fluorescence Sensing ◽  
Sulfate Reducing ◽  
Potential Applications ◽  
Reducing Bacteria

The development of H2S fluorescence-sensing strategies and their potential applications in the determination of sulfate-reducing bacteria activity.

Microbial control of the production of hydrogen sulfide by sulfate-reducing bacteria

1990 ◽  
Vol 35 (5) ◽  
pp. 533-539 ◽  
Author(s):  
Anne D. Montgomery ◽  
Michael J. Mclnerney ◽  
Kerry L. Sublette
Keyword(s):  
Hydrogen Sulfide ◽  
Microbial Control ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Production Of Hydrogen ◽  
Reducing Bacteria

scholarly journals The Influence of Hydrogeology to Generation of Hydrogen Sulfide of Low-Rank Coal in the Southeast Margin of Junggar Basin, China

Geofluids ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Qigen Deng ◽  
Tao Zhang ◽  
Fajun Zhao ◽  
Hao Wang ◽  
Jingping Yin
Keyword(s):  
Hydrogen Sulfide ◽  
High Salinity ◽  
Junggar Basin ◽  
Sulfate Reducing Bacteria ◽  
Low Rank ◽  
Low Rank Coal ◽  
Sulfate Reducing ◽  
Hydrogeological Characteristics ◽  
Reducing Bacteria ◽  
Coal Measures

The salinity, chemical properties, and migration characteristics of groundwater in coal measures are the key factors that affect the generation, migration, and reservoir of hydrogen sulfide (H2S) in low-rank coal seams. Taking the Jurassic coal and rock strata in the southeastern margin of the Junggar basin as the research object, according to the hydrogeological characteristics of the coal measures, the region is divided into 4 hydrogeological units. The coalbed methane contains a large number of secondary biogas. Along the direction of groundwater runoff, the salinity and the pH value increase gradually. The salinity in the hydrogeological units is low; it is not conducive to the propagation of sulfate-reducing bacteria and the formation of hydrogen sulfide of the Houxia, the south of Manasi River, and Hutubi and Liuhuangou area, the western region of the Miquan. The high salinity center and depressions of low water level (hydrodynamic stagnation zone) in the hydrogeological unit of the Liuhuanggou and the Miquan are the main areas for the production and enrichment of H2S in the low-rank coal. The high salinity in water is formed by infiltration, runoff, and drought evaporation. At the same time, the deep confined water environment closed well; in conditions of hydrocarbon-rich, under the action of sulfate-reducing bacteria, bacterial sulfate reduction will occur and hydrogen sulfide formed. According to the circulation characteristics of water bearing H2S in the region, imbricate and single bevel two kind generation and enrichment mode of hydrogen sulfide under the action of hydrodynamic control. The solubility of hydrogen sulfide in pure water and solutions of NaCl and Na2SO4 with different molar concentrations was calculated. The H2S solubility of groundwater in coal measures of 4 hydrogeological units was estimated.

Effect of hydrogen sulfide on growth of sulfate reducing bacteria

1992 ◽  
Vol 40 (5) ◽  
pp. 593-600 ◽  
Author(s):  
M. A. M. Reis ◽  
J. S. Almeida ◽  
P. C. Lemos ◽  
M. J. T. Carrondo
Keyword(s):  
Hydrogen Sulfide ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Reducing Bacteria

scholarly journals Thermophilic sulfate-reducing bacteria Moorela thermoacetica Nadia-3, isolated from “Nadiia” pit spoil heap of Chervonohrad mining region

2021 ◽  
Vol 15 (2) ◽  
pp. 35-46
Author(s):  
O. M. Сhayka ◽  
◽  
T. B. Peretyatko ◽  
A. A. Halushka ◽  
Keyword(s):  
Hydrogen Sulfide ◽  
Metal Ions ◽  
Organic Compounds ◽  
Elemental Sulfur ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Spoil Heap ◽  
Mining Region ◽  
Living Organisms ◽  
Reducing Bacteria

Introduction. Thermophilic sulfate-reducing bacteria attract attention of scientists as the potential agents of purification of wastewater polluted by sulfur and its compounds, heavy metal ions and organic compounds. These bacteria oxidize different organic substrates using metals with variable valency as electron acceptors and transform them into non-toxic or less toxic forms for living organisms. However, wastewater contains high concentrations of different toxic xenobiotics, particularly, metal ions that have negative influence on living organisms. For this reason, it is important to use resistant strains of microorganisms for the purification of wastewater. The aim of this work was to identify the thermophilic sulfur-reducing bacteria, isolated from “Nadiia” pit spoil heap of Chervonohrad mining region, and to study their properties. Materials and Methods. Thermophilic sulfur-reducing bacteria were isolated from the samples of rock of “Nadiia” pit heap at 50 cm depth. Bacteria were cultivated in TF medium under the anaerobic conditions in anaerostates. Cell biomass was measured turbidimetrically using the photoelectric colorimeter KFK-3 (λ = 340 nm, 3 mm cuvette). Hydrogen sulfide content was measured photoelectrocolorymetrically by the production of methylene blue. Organic acids content was measured by high performance liquid chromatography. Cr(VI), Fe(III), Мn(IV) and NO3– content was measured turbidimetrically. Results. Thermophilic sulfur-reducing bacteria were isolated from the rock of “Nadiia” pit heap of Chervonohrad mining region. They were identified as Moorela thermoacetica based on the morpho-physiological and biochemical properties and on the results of phylogenetic analysis. M. thermoacetica Nadia-3 grow in the synthetic TF medium, have the shape of elongated rods, are gram-positive, endospore-forming. They form light brown colonies. Optimal growth was observed at 50–55 °C, pH 6.5–7. The bacteria utilize glucose, starch, fructose, maltose, lactose, sodium lactate, arabinose, cellulose, maltose, glycerol, fumarate, and ethanol as carbon sources. The highest sulfidogenic activity of M. thermoacetica Nadia-3 was found in media with glycerol, lactose, and glucose. M. thermoacetica Nadia-3 reduce SO42-, S2O32-, Fe(III), NO3–, Cr(VI) compounds besides elemental sulfur. They accumulate biomass at K2Cr2O7 concentrations of 0.1–1 mM. Sulfur reduction is not the main way of energy accumulation. Conclusions. Thermophilic chromium-resistant sulfur-reducing bacteria M. thermoacetica Nadia-3, that produce hydrogen sulfide during the oxidation of different organic compounds, were isolated from the rock of “Nadiia” pit heap. They reduce Fe(III), Cr(VI), NO3–, SO42-, S2O32-, besides elemental sulfur.

scholarly journals Sulfidogenic activity of bacteria Desulfomicrobium sp. CrR3 under the influence of nitrite and sodium molybdate

2016 ◽  
pp. 131-135
Author(s):  
L. Dorosh ◽  
T. Peretiatko ◽  
S. Gudz
Keyword(s):  
Hydrogen Sulfide ◽  
Sodium Molybdate ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Complete Inhibition ◽  
Simultaneous Action ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
The City

Sulfate reducing bacteria Desulfomicrobium sp. CrR3, selected from the wastewater of the city, do not accumulate biomass under the influence of nitrite and molybdate ions. These substances inhibit sulfidogenic activity of bacteria Desulfomicrobium sp. CrR3. Additon of nitrite at a concentration of 1 mM has virtually no effect on the accumulation of biomass and hydrogen sulfide by bacteria Desulfomicrobium sp. CrR3. The increasing the concentration of nitrite up to 5 mM biomass accumulation of bacteria Desulfomicrobium sp. CrR3 was inhibited by approximately 50% while the observed decrease in efficiency of sulfate compared  with control. Molybdate at a concentration of 0.5–1 mM completely inhibits the growth and sulfidogenic activity of Desulfomicrobium sp. CrR3. Simultaneous action of nitrite and molybdate causes almost complete inhibition of Desulfomicrobium sp. CrR3 growth. Under these conditions the bacteria do not reduced sulfate and as a result do not accumulate hydrogen sulfide.

scholarly journals Destruction of toluene and xylene by sulfatе-reducing bacteria

2019 ◽  
Vol 30 (2) ◽  
pp. 95-100 ◽  
Author(s):  
N. S. Verkholiak ◽  
T. B. Peretyatko
Keyword(s):  
Hydrogen Sulfide ◽  
Aromatic Hydrocarbons ◽  
Aromatic Compounds ◽  
Sulfate Reducing Bacteria ◽  
Control Medium ◽  
Sulfate Ion ◽  
Sulfate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Growth Of Bacteria

As a result of human activity aromatic hydrocarbons enter the environment in large quantities, contaminating it. Dropping of insufficiently treated wastewater drains considerably decrease the quality of water. Quite effective biological methods of purification of contaminated environment are the usage of microorganisms. Prospective microorganisms for sewage treatment are sulfate-reducing bacteria. The purpose of the work was to investigate the ability of sulfate-reducing bacteria to use xylene and toluene as a source of carbon under different cultivation conditions.  The study objects were sulfate-reducing bacteria Desulfotomaculum AR1 and Desulfovibrio desulfuricans Ya-11. The biomass of bacteria was determined turbidimetrically, the content of sulfate ion and hydrogen sulfide – photometrically in the culture fluid. The content of fumarate was determined by the method of high-performance liquid chromatography. The ability of Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria to use toluene and xylene as the sole source of carbon and energy has been established. In the toluene environment, a better growth of bacteria was observed. A comparison was made between the efficiency of the reduction of sulfate ions and the growth of bacteria in the control medium and the medium with aromatic compounds. The efficiency of sulfate ions reduction was sufficiently high in the control medium, whereas in the toluene/xylene media the efficiency of sulfate utilization and hydrogen sulfide accumulation was lower compared to the control parameters. The growth of Desulfotomaculum AR1 and D. desulfuricans Ya-11 sulfate-reducing bacteria was investigated in xylene and toluene media in the presence/absence of fumarate. According to the results of the studies, the best growth of the tested bacteria was observed in the medium with aromatic compounds in the presence of fumarate and sulfate ion. Efficiency of use of fumarate in the medium with toluene with bacteria Desulfotomaculum AR1 was more than 90 %. Fumarate can be used by sulfate-reducing bacteria as a source of carbon, donor and acceptor of electrons. Fumarate is most likely to inhibit sulfate reduction in Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria, as indicated by studies showing that sulfate reduction efficiency in lactate, fumarate, and sulfate ion media was half that of control. Sulfate-reducing bacteria are capable to anaerobically degrade aromatic hydrocarbons in the presence of sulfate ions as terminal electron acceptors. The ability of sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 to use the aromatics of the BTEХ – toluene, xylene and sulfate-ions, makes them promising at the biological purification step of contaminated wastewater from these pollutants.

A selective near-infrared fluorescent probe for hydrogen sulfide and its application in sulfate-reducing bacteria detection

2016 ◽  
Vol 8 (16) ◽  
pp. 3339-3344 ◽  
Author(s):  
Peng Qi ◽  
Dun Zhang ◽  
Yan Sun ◽  
Yi Wan
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Sulfate Reducing Bacteria ◽  
Bacteria Detection ◽  
Sulfate Reducing ◽  
Reducing Bacteria

A novel NIR fluorescent probe for sulfide was presented, and applied in fluorescence imaging and detection of sulfate-reducing bacteria.

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