Vanishing Viral Cytopathic Effect Diagnoses in the Cytopatholoogy Laboratory

The structure-activity relationships of monomeric and dimeric macrocyclic polyamines were studied in an attempt to find potent inhibitors of human immunodeficiency virus (HIV) types 1 and 2. In general, dimeric polyamines are superior as HIV inhibitors to their monomeric counterparts, and the activity of a dimer is proportional to that of its constituent monomers. For the monomeric compounds, the amount of positive charge on the monomer rings under physiological conditions was more important for anti-HIV activity than the ring size. On the basis of these findings, the 14-membered tetraamine cyclam was selected as the component of dimeric compounds with potentially high activity. Of the series of newly synthesized bicyclams, in which the monomeric cyclams were linked at each C-6 position, a compound with an aIkyI chain bridge three carbons in length was found to exhibit the maximum anti-HIV activity. For one particular strain (HIV-2GH-1), syncytium formation was inhibited by the bicyclams at a similar concentration to that required to inhibit the viral cytopathic effect.

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Synthesis and Evaluation of Anti-Rhinovirus 1B Activity of Oxazolinyl-Isoflavans and -3(2H)-Isoflavenes

Antiviral Chemistry and Chemotherapy ◽

10.1177/095632029200300402 ◽

1992 ◽

Vol 3 (4) ◽

pp. 195-202 ◽

Cited By ~ 16

Author(s):

N. Desideri ◽

C. Conti ◽

I. Sestili ◽

P. Tomao ◽

M. L. Stein ◽

...

Keyword(s):

Carboxylic Acid ◽

Antiviral Activity ◽

Cytopathic Effect ◽

Potent Inhibitor ◽

Therapeutic Index ◽

Intermediate Compound ◽

Plaque Formation ◽

Interesting Compound ◽

Viral Cytopathic Effect

Oxazolinyl-isoflavans and −3(2H)-isoflavenes, substituted or not with a chlorine atom, were synthesized in order to compare their anti-rhinovirus activity with that of previously studied analogous compounds. The activity of the oxazolines and of the esters and acids, which are intermediates in the synthesis, was studied in vitro against rhinovirus serotype 1B infection in HeLa cells. The ability of various non cytotoxic concentrations to interfere with the development of the viral cytopathic effect and plaque formation was examined. All the tested compounds exerted a significant antiviral activity, and most of them were as active as some representative compounds of the oxazolinyl-phenoxyalkylisoxazole (WIN) series. 6-Oxazolinylisoflavan (VI) appeared to be the most interesting compound due to its high activity and therapeutic index. Among the substituted isoflavans and isoflavenes tested so far, the intermediate compound 6-chloro-3 (2H)-isoflavene-4′-carboxylic acid (XIX) was unexpectedly the most potent inhibitor of rhinovirus 1B plaque formation.

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Efficacy of microbicides for inactivation of Ebola–Makona virus on a non-porous surface: a targeted hygiene intervention for reducing virus spread

Scientific Reports ◽

10.1038/s41598-020-71736-x ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Todd A. Cutts ◽

Catherine Robertson ◽

Steven S. Theriault ◽

Raymond W. Nims ◽

Samantha B. Kasloff ◽

...

Keyword(s):

Infection Prevention ◽

Ebola Virus ◽

Cytopathic Effect ◽

Infectious Virus ◽

Hard Water ◽

Infection Prevention And Control ◽

Environmental Surfaces ◽

Hygiene Intervention ◽

And Control ◽

Viral Cytopathic Effect

Abstract Microbicides play critical roles in infection prevention and control of Ebola virus by decontaminating high-touch environmental surfaces (HITES), interrupting the virus-HITES-hands nexus. We evaluated the efficacy of formulations containing different microbicidal actives for inactivating Ebola virus–Makona strain (EBOV/Mak) on stainless-steel carriers per ASTM E2197-11. Formulations of sodium hypochlorite (NaOCl) (0.05–1%), ethanol (70%), chloroxylenol (PCMX) (0.12–0.48% by weight) in hard water, and a ready-to-use disinfectant spray with 58% ethanol (EDS), were tested at contact times of 0, or 0.5 to 10 min at ambient temperature. EBOV/Mak was inactivated (> 6 log10) by 70% ethanol after contact times ≥ 2.5 min, by 0.5% and 1% NaOCl or EDS (> 4 log10) at contact times ≥ 5 min, and by 0.12–0.48% PCMX (> 4.2 log10) at contact times ≥ 5 min. Residual infectious virus in neutralized samples was assessed by passage on cells and evaluation for viral cytopathic effect. No infectious virus was detected in cells inoculated with EBOV/Mak exposed to NaOCl (0.5% or 1%), PCMX (0.12% to 0.48%), or EDS for ≥ 5 min. These results demonstrate ≥ 6 log10 inactivation of EBOV/Mak dried on prototypic surfaces by EDS or formulations of NaOCl (≥ 0.5%), PCMX (≥ 0.12%), or 70% ethanol at contact times ≥ 5 min.

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Significance of decoy cells for indicating viral cytopathic effect in urine cytology

Cytopathology ◽

10.1111/cyt.12261 ◽

2015 ◽

Vol 27 (3) ◽

pp. 220-221 ◽

Cited By ~ 2

Author(s):

M. Kimura ◽

T. Hayashi

Keyword(s):

Cytopathic Effect ◽

Urine Cytology ◽

Viral Cytopathic Effect

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Assay of human interferon in Vero cells by several methods

Journal of Clinical Microbiology ◽

10.1128/jcm.9.4.471-475.1979 ◽

1979 ◽

Vol 9 (4) ◽

pp. 471-475

Author(s):

P C Ferreira ◽

M L Peixoto ◽

M A Silva ◽

R R Golgher

Keyword(s):

Vesicular Stomatitis Virus ◽

Sindbis Virus ◽

Reduction Method ◽

Cytopathic Effect ◽

Vero Cells ◽

Human Interferon ◽

Inhibition Assay ◽

Vesicular Stomatitis ◽

Viral Cytopathic Effect

Four methods for the assay of human interferon in Vero cells were compared based on the inhibition of viral cytopathic effect (CPE) in tubes, the inhibition of CPE in microplates, the reduction of plaques, and the inhibition of quantitative hemadsorption. For inhibition of CPE, Sindbis virus, vesicular stomatitis virus, poliovirus type 2, and vaccinia virus were used for challenge. In the plaque reduction method, Sindbis virus, vesicular stomatitis virus, and poliovirus were employed, and Newcastle disease virus was used in the quantitative hemadsorption assay. Sindbis virus was most susceptible to interferon in those tests measuring inhibition of CPE, but vesicular stomatitis virus was as sensitive in the plaque reduction method. Highest titers of interferon were recorded in microplates, especially with Sindbis virus as the challenge agent, followed by the quantitative inhibition assay. The CPE inhibition method was the simplest, and the quantitative hemadsorption assay was the most rapid to perform. Reproducibilities, as shown by the coefficient of variation, were 15, 39, and 59% for plaque reduction, CPE inhibition in tubes, and CPE inhibition in microplates, respectively.

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Interaction between Tubulin and the Viral Matrix Protein of Vesicular Stomatitis Virus: Possible Implications in the Viral Cytopathic Effect

Virology ◽

10.1006/viro.1994.1350 ◽

1994 ◽

Vol 202 (1) ◽

pp. 339-347 ◽

Cited By ~ 46

Author(s):

Ronald Melki ◽

Yves Gaudin ◽

Danielle Blondel

Keyword(s):

Vesicular Stomatitis Virus ◽

Matrix Protein ◽

Cytopathic Effect ◽

Vesicular Stomatitis ◽

Viral Cytopathic Effect

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ATeam technology for detecting early signs of viral cytopathic effect

Journal of Veterinary Medical Science ◽

10.1292/jvms.20-0021 ◽

2020 ◽

Vol 82 (3) ◽

pp. 387-393

Author(s):

Karla Cristine C. DOYSABAS ◽

Mami OBA ◽

Tomoki ISHIBASHI ◽

Hideki SHIBATA ◽

Hitoshi TAKEMAE ◽

...

Keyword(s):

Cytopathic Effect ◽

Early Signs ◽

Viral Cytopathic Effect

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Human Fibroblast Interferon Adjuvant to CO2 Laser in the Treatment of Recurrent Juvenile Laryngeal Papillomatosis: Experience with 7 Cases

Tumori Journal ◽

10.1177/030089168907500314 ◽

1989 ◽

Vol 75 (3) ◽

pp. 259-262 ◽

Cited By ~ 2

Author(s):

Fausto Chiesa ◽

Rosangela Donghi ◽

Silvana Pilotti ◽

Luigi Sala ◽

Bernardina Stefanon

Keyword(s):

Complete Remission ◽

Diagnostic Procedure ◽

Cytopathic Effect ◽

Human Fibroblasts ◽

Combined Treatment ◽

Laryngeal Papillomatosis ◽

Cirrhotic Patients ◽

Laser Excision ◽

Viral Cytopathic Effect

Preliminary results of adjuvant human fibroblasts interferon (IFN beta) given after CO2 laser excision in recurrent laryngeal papillomatosis in 7 adult patients are reported. Diagnostic procedure included histologic and immunohistochemical investigations to demonstrate the presence of viral cytopathic effect and for characterization of the virus. All patients underwent CO2 laser excision under general anesthesia followed by administration of IFN beta intramuscularly at the dose of 4x106 IU/day for 10 consecutive days. In the presence of complete remission, patients were followed without further therapy; in the presence of partial remission, a new combined treatment was established. All patients had a complete remission after combined treatment, but 4 subsequently developed recurrences. Treatments were always well tolerated; even cirrhotic patients showed no side effects.

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Evaluation of Urine SARS-COV-2 RT-PCR as a predictor of Acute Kidney Injury and disease severity in critical COVID-19 patients

10.1101/2021.01.13.21249576 ◽

2021 ◽

Author(s):

Sérgio Pinto de Souza ◽

Marcelo Augusto Duarte Silveira ◽

Bruno Solano de Freitas Souza ◽

Carolina Kymie Vasques Nonaka ◽

Erica de Melo ◽

...

Keyword(s):

Respiratory Function ◽

Cytopathic Effect ◽

Severe Disease ◽

Genetic Material ◽

Kidney Injury ◽

The Novel ◽

Disease Phenotype ◽

Rt Pcr ◽

Novel Coronavirus ◽

Viral Cytopathic Effect

AbstractThe novel coronavirus disease (COVID-19) is an emerging infectious disease caused by SARS-CoV-2, which began as an outbreak in Wuhan, China and spread rapidly throughout the globe. Although the majority of infections are mild, severe and critical COVID-19 patients face deterioration of respiratory function, and may also present extrapulmonary manifestations, mostly affecting the kidney, digestive tract, heart and nervous system. Here, we prospectively evaluated the presence of SARS-CoV-2 genetic material by RT-PCR in urine samples obtained from critical care COVID-19 patients. In 51 patients included, we found higher serum creatinine levels, a longer hospital stay and a more frequent dialysis need in urine-positive patients. These findings could suggest that, in predisposed patients, a direct viral cytopathic effect may contribute to a more severe disease phenotype.

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