Mapping soil deformation around plant roots using in vivo 4D X-ray Computed Tomography and Digital Volume Correlation

Abstract Aims We sought to develop a novel experimental system which enabled application of iodinated contrast media to in vivo plant roots intact in soil and was compatible with time-resolved synchrotron X-ray computed tomography imaging. The system was developed to overcome issues of low contrast to noise within X-ray computed tomography images of plant roots and soil environments, the latter of which can complicate image processing and result in the loss of anatomical information. Methods To demonstrate the efficacy of the system we employ the novel use of both synchrotron X-ray computed tomography and synchrotron X-ray fluorescence mapping to capture the translocation of the contrast media through root vasculature into the leaves. Results With the application of contrast media we identify fluid flow in root vasculature and visualise anatomical features, which are otherwise often only observable in ex vivo microscopy, including: the xylem, metaxylem, pith, fibres in aerenchyma and leaf venation. We are also able to observe interactions between aerenchyma cross sectional area and solute transport in the root vasculature with depth. Conclusions Our novel system was capable of successfully delivering sufficient contrast media into root and leaf tissues such that anatomical features could be visualised and internal fluid transport observed. We propose that our system could be used in future to study internal plant transport mechanisms and parameterise models for fluid flow in plants.

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PEG modified BaGdF5:Yb/Er nanoprobes for multi-modal upconversion fluorescent, in vivo X-ray computed tomography and biomagnetic imaging

Biomaterials ◽

10.1016/j.biomaterials.2012.09.019 ◽

2012 ◽

Vol 33 (36) ◽

pp. 9232-9238 ◽

Cited By ~ 199

Author(s):

Songjun Zeng ◽

Ming-Kiu Tsang ◽

Chi-Fai Chan ◽

Ka-Leung Wong ◽

Jianhua Hao

Keyword(s):

Computed Tomography ◽

X Ray ◽

X Ray Computed ◽

Biomagnetic Imaging

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Multi-scale X-ray computed tomography to detect and localize metal-based nanomaterials in lung tissues of in vivo exposed mice

Scientific Reports ◽

10.1038/s41598-018-21862-4 ◽

2018 ◽

Vol 8 (1) ◽

Cited By ~ 7

Author(s):

Perrine Chaurand ◽

Wei Liu ◽

Daniel Borschneck ◽

Clément Levard ◽

Mélanie Auffan ◽

...

Keyword(s):

Computed Tomography ◽

X Ray ◽

Multi Scale ◽

X Ray Computed

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Evaluation of Regenerative Processes in a Rat Model of Mandibular Condyle Defect using in vivo Micro X-Ray Computed Tomography

Journal of Hard Tissue Biology ◽

10.2485/jhtb.21.407 ◽

2012 ◽

Vol 21 (4) ◽

pp. 407-412 ◽

Cited By ~ 1

Author(s):

Tadahito Saito ◽

Takayuki Mashimo ◽

Hiroshi Shiratsuchi ◽

Shunsuke Namaki ◽

Kunihito Matsumoto ◽

...

Keyword(s):

Computed Tomography ◽

Rat Model ◽

Mandibular Condyle ◽

Regenerative Processes ◽

X Ray ◽

X Ray Computed

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Comparison of the Mechanical Behaviour of Standard and Auxetic Foams by X-ray Computed Tomography and Digital Volume Correlation

Strain ◽

10.1111/str.12053 ◽

2013 ◽

Vol 49 (6) ◽

pp. 467-482 ◽

Cited By ~ 26

Author(s):

F. Pierron ◽

S. A. McDonald ◽

D. Hollis ◽

J. Fu ◽

P. J. Withers ◽

...

Keyword(s):

Computed Tomography ◽

Mechanical Behaviour ◽

Digital Volume Correlation ◽

X Ray ◽

X Ray Computed

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A High-Performance Ytterbium-Based Nanoparticulate Contrast Agent for In Vivo X-Ray Computed Tomography Imaging

Angewandte Chemie ◽

10.1002/ange.201106686 ◽

2011 ◽

Vol 124 (6) ◽

pp. 1466-1471 ◽

Cited By ~ 15

Author(s):

Yanlan Liu ◽

Kelong Ai ◽

Jianhua Liu ◽

Qinghai Yuan ◽

Yangyang He ◽

...

Keyword(s):

Computed Tomography ◽

Contrast Agent ◽

High Performance ◽

Computed Tomography Imaging ◽

X Ray ◽

Tomography Imaging ◽

X Ray Computed

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Quantitative 3D strain analysis in analogue experiments simulating tectonic deformation: Integration of X-ray computed tomography and digital volume correlation techniques

Journal of Structural Geology ◽

10.1016/j.jsg.2013.07.011 ◽

2013 ◽

Vol 55 ◽

pp. 127-149 ◽

Cited By ~ 42

Author(s):

J. Adam ◽

M. Klinkmüller ◽

G. Schreurs ◽

B. Wieneke

Keyword(s):

Computed Tomography ◽

Strain Analysis ◽

Tectonic Deformation ◽

Digital Volume Correlation ◽

X Ray ◽

X Ray Computed ◽

Correlation Techniques ◽

Analogue Experiments

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Understanding the mechanisms of root-reinforcement in soils: soil shear tests using X-ray computed tomography and digital volume correlation

E3S Web of Conferences ◽

10.1051/e3sconf/20199212009 ◽

2019 ◽

Vol 92 ◽

pp. 12009

Author(s):

Daniel Bull ◽

Ian Sinclair ◽

Fabrice Pierron ◽

Tiina Roose ◽

Joel Smethurst

Keyword(s):

Computed Tomography ◽

Three Dimensions ◽

Digital Volume Correlation ◽

Strain Component ◽

Multiscale Models ◽

Root Reinforcement ◽

Length Scales ◽

X Ray ◽

Shearing Resistance ◽

X Ray Computed

Soil containing plant roots may be expected to exhibit a greater shearing resistance compared with the same ‘unreinforced’ soil, providing enhanced stability and effective erosion control, particularly for earth slopes. To be able to rely on the improved shearing resistance and stiffness of root-reinforced soils, it is important to understand and quantify the effectiveness of root reinforcement. This requires sophisticated multiscale models, building understanding at different length scales, from individual soil-root interaction through to full soil-profile or slope scale. One of the challenges with multiscale models is ensuring that they are representative of real behaviour, and this requires calibration to detailed high-quality experiments. The focus of the work presented was to capture and quantify root-reinforcement behaviour and associated soil and root deformation mechanisms during direct shear at the macroscopic to millimetre length scales. A novel shear box was developed to operate within a large-scale X-ray computed tomography (CT) scanner. Tests were interrupted to be scanned at a series of shear displacements from 0-20 mm to capture the chronology of behaviour in three-dimensions. Digital volume correlation (DVC) was applied to the CT dataset to obtain full-field 3D displacement and strain component information. The study demonstrates feasibility of the technique and presents preliminary DVC results.

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Mineralization of Sialoliths Investigated by Ex Vivo and In Vivo X-ray Computed Tomography

Microscopy and Microanalysis ◽

10.1017/s1431927618016124 ◽

2019 ◽

Vol 25 (1) ◽

pp. 151-163 ◽

Cited By ~ 1

Author(s):

Pedro Nolasco ◽

Paulo V. Coelho ◽

Carla Coelho ◽

David F. Angelo ◽

J. R. Dias ◽

...

Keyword(s):

Electron Microscopy ◽

Computed Tomography ◽

Organic Matter ◽

Ex Vivo ◽

Correct Selection ◽

Quantitative Relation ◽

Helical Computed Tomography ◽

X Ray ◽

X Ray Computed

AbstractThe fraction of organic matter present affects the fragmentation behavior of sialoliths; thus, pretherapeutic information on the degree of mineralization is relevant for a correct selection of lithotripsy procedures. This work proposes a methodology for in vivo characterization of salivary calculi in the pretherapeutic context. Sialoliths were characterized in detail by X-ray computed microtomography (μCT) in combination with atomic emission spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. Correlative analysis of the same specimens was performed by in vivo and ex vivo helical computed tomography (HCT) and ex vivo μCT. The mineral matter in the sialoliths consisted essentially of apatite (89 vol%) and whitlockite (11 vol%) with average density of 1.8 g/cm3. In hydrated conditions, the mineral mass prevailed with 53 ± 13 wt%, whereas the organic matter, with a density of 1.2 g/cm3, occupied 65 ± 10% of the sialoliths’ volume. A quantitative relation between sialoliths mineral density and X-ray attenuation is proposed for both HCT and μCT.

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