Variation of the microbial community in thermophilic anaerobic digestion of pig manure mixed with different ratios of rice straw

2016 ◽  
Vol 122 (3) ◽  
pp. 334-340 ◽  
Author(s):  
Sheng Zhou ◽  
Marcell Nikolausz ◽  
Jining Zhang ◽  
Shohei Riya ◽  
Akihiko Terada ◽  
...  
2018 ◽  
Vol 76 ◽  
pp. 350-356 ◽  
Author(s):  
Shohei Riya ◽  
Kazuhiro Suzuki ◽  
Lingyu Meng ◽  
Sheng Zhou ◽  
Akihiko Terada ◽  
...  

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Sheng Zhou ◽  
Jining Zhang ◽  
Guoyan Zou ◽  
Shohei Riya ◽  
Masaaki Hosomi

To evaluate the feasibility of swine manure treatment by a proposed Dry Thermophilic Anaerobic Digestion (DT-AD) system, we evaluated the methane yield of swine manure treated using a DT-AD method with rice straw under different C/N ratios and solid retention time (SRT) and calculated the mass and energy balances when the DT-AD system is used for swine manure treatment from a model farm with 1000 pigs and the digested residue is used for forage rice production. A traditional swine manure treatment Oxidation Ditch system was used as the study control. The results suggest that methane yield using the proposed DT-AD system increased with a higher C/N ratio and shorter SRT. Correspondently, for the DT-AD system running with SRT of 80 days, the net energy yields for all treatments were negative, due to low biogas production and high heat loss of digestion tank. However, the biogas yield increased when the SRT was shortened to 40 days, and the generated energy was greater than consumed energy when C/N ratio was 20:1 and 30:1. The results suggest that with the correct optimization of C/N ratio and SRT, the proposed DT-AD system, followed by using digestate for forage rice production, can attain energy self-sufficiency.


2008 ◽  
Vol 57 (8) ◽  
pp. 1199-1205 ◽  
Author(s):  
T. Kobayashi ◽  
Y. Y. Li ◽  
H. Harada

The microbial community structures in the thermophilic anaerobic digestion (TAD) of waste activated sludge (WAS) and WAS were analyzed with molecular biological techniques including real-time polymerase chain reaction (PCR) and cloning. The microbial community of TAD had less diversity than that of WAS, and the sequences obtained in WAS were not present in TAD by the cloning analysis. In the TAD bacterial clone library, 97.5% of total clones were affiliated with the phylum Firmicutes and 73.1% with the genus Coprothermobacter. Real-time PCR and cloning analysis revealed that the number of Methanosarcina thermophila, which is an acetoclastic methanogen, is larger than that of Methanoculleus thermophilus, which is a hydrogenotrophic methanogen, in terms of the numbers of copies of 16S ribosomal DNA (rDNA).


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