Upgrading and detoxification of aqueous extracts from dry olive mill residues by white-rot fungi

Anaerobic digestion of olive-mill wastewater (OMW) was carried out in a continuously fed mode bioreactor. The bioreactor was operated at different hydraulic retention times (HRTs), using OMW, either raw or pre-treated with white-rot fungi. Two different kinds of feed were tested in this process, one which was thermally treated and subjected to sedimentation, in order to remove the solids contained and the other without any physicochemical treatment (raw OMW). Thermally pretreated OMW did not allow a stable operation even at an HRT of 30d. Further pretreatment of the OMW with a white-rot fungus for removal of the contained phenolics, allowed a stable operation at an HRT of 30 d. On the other hand, simple dilution of the raw wastewater, without any solids removal, lead to a stable operation at an HRT of 30d and was accompanied by higher production of biogas. The presence of the solids in the OMW proved to be a determining factor for the stability of the process and could be attributed to a possible adsorption on the solids of hydrophobic compounds, such as long-chain fatty acids that are toxic to methanogens.

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Organic matter evolution and partial detoxification in two-phase olive mill waste colonized by white-rot fungi

International Biodeterioration & Biodegradation ◽

10.1016/j.ibiod.2007.02.001 ◽

2007 ◽

Vol 60 (2) ◽

pp. 116-125 ◽

Cited By ~ 39

Author(s):

Inmaculada Sampedro ◽

Sara Marinari ◽

Alessandro D’Annibale ◽

Stefano Grego ◽

Juan A. Ocampo ◽

...

Keyword(s):

Organic Matter ◽

White Rot Fungi ◽

White Rot ◽

Two Phase ◽

Olive Mill Waste ◽

Mill Waste ◽

Olive Mill

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Screening of white rot fungi for the treatment of olive mill waste-waters

Journal of Chemical Technology & Biotechnology ◽

10.1002/jctb.280570208 ◽

2007 ◽

Vol 57 (2) ◽

pp. 141-146 ◽

Cited By ~ 59

Author(s):

Sami Sayadi ◽

Radhouane Ellouz

Keyword(s):

White Rot Fungi ◽

White Rot ◽

Waste Waters ◽

Olive Mill Waste ◽

Mill Waste ◽

Olive Mill

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Immobilized Inocula of White-Rot Fungi Accelerate both Detoxification and Organic Matter Transformation in Two-Phase Dry Olive-Mill Residue

Journal of Agricultural and Food Chemistry ◽

10.1021/jf900243k ◽

2009 ◽

Vol 57 (12) ◽

pp. 5452-5460 ◽

Cited By ~ 14

Author(s):

Inmaculada Sampedro ◽

Tomas Cajthaml ◽

Sara Marinari ◽

Silvia Rita Stazi ◽

Stefano Grego ◽

...

Keyword(s):

Organic Matter ◽

White Rot Fungi ◽

White Rot ◽

Two Phase ◽

Organic Matter Transformation ◽

Olive Mill

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Potential role of Bacillus endospores in soil amended by olive mill wastewater

Water Science & Technology ◽

10.2166/wst.2010.082 ◽

2010 ◽

Vol 61 (11) ◽

pp. 2873-2879 ◽

Cited By ~ 4

Author(s):

Gino Naclerio ◽

Antonio Falasca ◽

Emma Petrella ◽

Valentina Nerone ◽

Federica Cocco ◽

...

Keyword(s):

Potential Role ◽

Laccase Activity ◽

White Rot Fungi ◽

Olive Mill Wastewater ◽

White Rot ◽

Bacillus Species ◽

Italian Law ◽

Bacillus Spores ◽

Olive Mill

The main aim of this work was to know how spread is laccase activity in spores of Bacillus species isolated from a soil where Italian law allows olive mill wastewater (OMW) spreading, and to investigate the potential role of such autochthonous soil microorganisms in degradation of OMW phenols, and prevention of groundwater pollution. Laccase activity was detected for the first time in spores of wild-type Bacilluspumilus, B. cereus sensu lato, and B. amyloliquefaciens strains. Because B. pumilus, B. cereus sensu lato, and B. amyloliquefaciens, together with B. subtilis account for a total of 93% of Bacillus isolates at the study site, the nearly totality of Bacillus spores reveals laccase activity. Thus, taking also into consideration that Bacillus spores are more abundant (about 100-fold) than white-rot fungi (that possess a well known extracellular, radical-based ligninolytic enzyme system capable of degrading OMW phenols) in the studied soil, these spores may contribute to in-situ degradation of OMW phenols. This role is further emphasized by dilution of crude OMW during infiltration of rainwater through soil that allows to minimize the antibacterial activity of phenols. The widespread presence of Bacillus spores in soils indicates a potential detoxifying role of these spores in a broader context.

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