Olive mill wastewater biodegradation potential of white-rot fungi – Mode of action of fungal culture extracts and effects of ligninolytic enzymes

2015 ◽  
Vol 189 ◽  
pp. 121-130 ◽  
Author(s):  
Spyridon Ntougias ◽  
Petr Baldrian ◽  
Constantinos Ehaliotis ◽  
Frantisek Nerud ◽  
Věra Merhautová ◽  
...  
Keyword(s):  
Mode Of Action ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Olive Mill Wastewater ◽  
White Rot ◽  
Fungal Culture ◽  
Olive Mill

scholarly journals Anaerobic digestion of olive mill wastewater

2013 ◽  
Vol 11 (3) ◽  
pp. 364-372
Keyword(s):  
Anaerobic Digestion ◽  
White Rot Fungi ◽  
Olive Mill Wastewater ◽  
The Other ◽  
White Rot ◽  
White Rot Fungus ◽  
Stable Operation ◽  
The Stability ◽  
Solids Removal ◽  
Olive Mill

Anaerobic digestion of olive-mill wastewater (OMW) was carried out in a continuously fed mode bioreactor. The bioreactor was operated at different hydraulic retention times (HRTs), using OMW, either raw or pre-treated with white-rot fungi. Two different kinds of feed were tested in this process, one which was thermally treated and subjected to sedimentation, in order to remove the solids contained and the other without any physicochemical treatment (raw OMW). Thermally pretreated OMW did not allow a stable operation even at an HRT of 30d. Further pretreatment of the OMW with a white-rot fungus for removal of the contained phenolics, allowed a stable operation at an HRT of 30 d. On the other hand, simple dilution of the raw wastewater, without any solids removal, lead to a stable operation at an HRT of 30d and was accompanied by higher production of biogas. The presence of the solids in the OMW proved to be a determining factor for the stability of the process and could be attributed to a possible adsorption on the solids of hydrophobic compounds, such as long-chain fatty acids that are toxic to methanogens.

Potential role of Bacillus endospores in soil amended by olive mill wastewater

2010 ◽  
Vol 61 (11) ◽  
pp. 2873-2879 ◽  
Author(s):  
Gino Naclerio ◽  
Antonio Falasca ◽  
Emma Petrella ◽  
Valentina Nerone ◽  
Federica Cocco ◽  
...  
Keyword(s):  
Potential Role ◽  
Laccase Activity ◽  
White Rot Fungi ◽  
Olive Mill Wastewater ◽  
White Rot ◽  
Bacillus Species ◽  
Italian Law ◽  
Bacillus Spores ◽  
Olive Mill

The main aim of this work was to know how spread is laccase activity in spores of Bacillus species isolated from a soil where Italian law allows olive mill wastewater (OMW) spreading, and to investigate the potential role of such autochthonous soil microorganisms in degradation of OMW phenols, and prevention of groundwater pollution. Laccase activity was detected for the first time in spores of wild-type Bacilluspumilus, B. cereus sensu lato, and B. amyloliquefaciens strains. Because B. pumilus, B. cereus sensu lato, and B. amyloliquefaciens, together with B. subtilis account for a total of 93% of Bacillus isolates at the study site, the nearly totality of Bacillus spores reveals laccase activity. Thus, taking also into consideration that Bacillus spores are more abundant (about 100-fold) than white-rot fungi (that possess a well known extracellular, radical-based ligninolytic enzyme system capable of degrading OMW phenols) in the studied soil, these spores may contribute to in-situ degradation of OMW phenols. This role is further emphasized by dilution of crude OMW during infiltration of rainwater through soil that allows to minimize the antibacterial activity of phenols. The widespread presence of Bacillus spores in soils indicates a potential detoxifying role of these spores in a broader context.

scholarly journals Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 19
Author(s):  
Anastasia Zerva ◽  
Nikolaos Tsafantakis ◽  
Evangelos Topakas
Keyword(s):  
Biomass Conversion ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Inhibitory Effect ◽  
Biologically Active ◽  
White Rot ◽  
Oxidative Enzymes ◽  
Industrial Residues ◽  
Pure Compounds ◽  
Wild Strains

White-rot basidiomycetes are the only microorganisms with the ability to produce both hydrolytic (cellulases and hemicellulases) and oxidative (ligninolytic) enzymes for degrading cellulose/hemicellulose and lignin. In addition, they produce biologically active natural products with important application in cosmetic formulations, either as pure compounds or as standardized extracts. In the present work, three wild strains of Basidiomycetes fungi (Pleurotus citrinopileatus, Abortiporus biennis and Ganoderma resinaceum) from Greek habitats were grown in agro-industrial residues (oil mill wastewater, and corn cob) and evaluated for their anti-tyrosinase and antioxidant activity and for the production of biotechnologically relevant enzymes. P. citrinopileatus showed the most interesting tyrosinase inhibitory activity, while A. biennis showed the highest DPPH(2,2-diphenyl-1-picryl-hydrazyl) scavenging potential. Corn cobs were the most appropriate carbon source for maximizing the inhibitory effect of fungal biomasses on both activities, while the use of oil mill wastewater selectively increased the anti-tyrosinase potential of P. citrinopileatus culture filtrate. All strains were found to be preferential lignin degraders, similarly to most white-rot fungi. Bioinformatic analyses were performed on the proteome of the strains P. citrinopileatus and A. biennis, focusing on CAZymes with biotechnological relevance, and the results were compared with the enzyme activities of culture supernatants. Overall, all three strains showed strong production of oxidative enzymes for biomass conversion applications.

Distribution of ligninolytic enzymes in selected white-rot fungi

1996 ◽  
Vol 41 (3) ◽  
pp. 264-266 ◽  
Author(s):  
F. Nerud ◽  
Z. Mišurcová
Keyword(s):  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot

Mycoremediation of Lignocelluloses

Biotechnology ◽  
2019 ◽  
pp. 1086-1108
Author(s):  
Saritha Vara
Keyword(s):  
Spatial Distribution ◽  
Animal Feed ◽  
Space Exploration ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Mechanisms Of Action ◽  
White Rot ◽  
Lignocellulolytic Enzymes ◽  
Biotechnological Applications ◽  
Potential Use

The most abundant aromatic biopolymer on earth Lignin is extremely recalcitrant to degradation. It creates a barrier to solutions or enzymes by linking to both hemicellulose and cellulose preventing the penetration of lignocellulolytic enzymes into the interior lignocellulosic structure. Global attention has been gained by fungi owing to the potential use of their versatile enzymes for agriculture, medicines, industries and bioremediation. The combination of extracellular ligninolytic enzymes, mediators, organic acids and accessory enzymes make some of the basidiomycete white-rot fungi to be able to degrade lignin efficiently. This review describes remediation of lignocelluloses by fungi, properties of fungi, their spatial distribution and the mechanisms of action which render them attractive candidates in biotechnological applications like biopulping, animal feed, genetic engineering and space exploration.

Upgrading and detoxification of aqueous extracts from dry olive mill residues by white-rot fungi

2010 ◽  
Vol 150 ◽  
pp. 225-225
Author(s):  
I. Sampedro ◽  
F. Federici ◽  
J.A. Ocampo ◽  
I. Garcia Romera ◽  
A. D’Annibale ◽  
...  
Keyword(s):  
White Rot Fungi ◽  
White Rot ◽  
Aqueous Extracts ◽  
Mill Residues ◽  
Olive Mill

scholarly journals Induction of Extracellular Hydroxyl Radical Production by White-Rot Fungi through Quinone Redox Cycling

2009 ◽  
Vol 75 (12) ◽  
pp. 3944-3953 ◽  
Author(s):  
Víctor Gómez-Toribio ◽  
Ana B. García-Martín ◽  
María J. Martínez ◽  
Ángel T. Martínez ◽  
Francisco Guillén
Keyword(s):  
Hydroxyl Radical ◽  
Fenton Reaction ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot ◽  
Oh Radicals ◽  
Production Rates ◽  
Pycnoporus Cinnabarinus ◽  
Simple Strategy ◽  
The One

ABSTRACT A simple strategy for the induction of extracellular hydroxyl radical (OH) production by white-rot fungi is presented. It involves the incubation of mycelium with quinones and Fe3+-EDTA. Succinctly, it is based on the establishment of a quinone redox cycle catalyzed by cell-bound dehydrogenase activities and the ligninolytic enzymes (laccase and peroxidases). The semiquinone intermediate produced by the ligninolytic enzymes drives OH production by a Fenton reaction (H2O2 + Fe2+ → OH + OH− + Fe3+). H2O2 production, Fe3+ reduction, and OH generation were initially demonstrated with two Pleurotus eryngii mycelia (one producing laccase and versatile peroxidase and the other producing just laccase) and four quinones, 1,4-benzoquinone (BQ), 2-methoxy-1,4-benzoquinone (MBQ), 2,6-dimethoxy-1,4-benzoquinone (DBQ), and 2-methyl-1,4-naphthoquinone (menadione [MD]). In all cases, OH radicals were linearly produced, with the highest rate obtained with MD, followed by DBQ, MBQ, and BQ. These rates correlated with both H2O2 levels and Fe3+ reduction rates observed with the four quinones. Between the two P. eryngii mycelia used, the best results were obtained with the one producing only laccase, showing higher OH production rates with added purified enzyme. The strategy was then validated in Bjerkandera adusta, Phanerochaete chrysosporium, Phlebia radiata, Pycnoporus cinnabarinus, and Trametes versicolor, also showing good correlation between OH production rates and the kinds and levels of the ligninolytic enzymes expressed by these fungi. We propose this strategy as a useful tool to study the effects of OH radicals on lignin and organopollutant degradation, as well as to improve the bioremediation potential of white-rot fungi.

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