Robust on-line sampling and analysis during long-term perfusion cultivation of mammalian cells

We develop a sampling-based motion planning algorithm that combines long-term temporal logic goals with short-term reactive requirements. The mission specification has two parts: (1) a global specification given as a linear temporal logic (LTL) formula over a set of static service requests that occur at the regions of a known environment, and (2) a local specification that requires servicing a set of dynamic requests that can be sensed locally during the execution. The proposed computational framework consists of two main ingredients: (a) an off-line sampling-based algorithm for the construction of a global transition system that contains a path satisfying the LTL formula; and (b) an on-line sampling-based algorithm to generate paths that service the local requests, while making sure that the satisfaction of the global specification is not affected. The off-line algorithm has four main features. First, it is incremental, in the sense that the procedure for finding a satisfying path at each iteration scales only with the number of new samples generated at that iteration. Second, the underlying graph is sparse, which implies low complexity for the overall method. Third, it is probabilistically complete. Fourth, under some mild assumptions, it has the best possible complexity bound. The on-line algorithm leverages ideas from LTL monitoring and potential functions to ensure progress towards the satisfaction of the global specification while servicing locally sensed requests. Examples and experimental trials illustrating the usefulness and the performance of the framework are included.

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Aspects of the Evolution of the Romanian Tourists’ Preferences Concerning the Domestic Tourist Destinations

Valahian Journal of Economic Studies ◽

10.2478/vjes-2019-0002 ◽

2019 ◽

Vol 10 (1) ◽

pp. 21-28

Author(s):

Aniela Bălăcescu ◽

Radu Șerban Zaharia

Keyword(s):

Logistic Model ◽

Local Level ◽

Medium Term ◽

Tourist Destinations ◽

Tourism Management ◽

Production And Consumption ◽

On Line ◽

The One ◽

Long Term Studies

Abstract Tourist services represent a category of services in which the inseparability of production and consumption, the inability to be storable, the immateriality, and last but not least non-durability, induces in tourism management a number of peculiarities and difficulties. Under these circumstances the development of medium-term strategies involves long-term studies regarding on the one hand the developments and characteristics of the demand, and on the other hand the tourist potential analysis at regional and local level. Although in the past 20 years there has been tremendous growth of on-line booking made by household users, the tour operators agencies as well as those with sales activity continue to offer the specific services for a large number of tourists, that number, in the case of domestic tourism, increased by 1.6 times in case of the tour operators and by 4.44 times in case of the agencies with sales activity. At the same time, there have been changes in the preferences of tourists regarding their holiday destinations in Romania. Started on these considerations, paper based on a logistic model, examines the evolution of the probabilities and scores corresponding to the way the Romanian tourists spend their holidays on the types of tourism agencies, actions and tourist areas in Romania.

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A Study of Standardization of the Aptitude Tests Using Item Parameters Obtained by On-line Sampling Methods

Journal of Curriculum and Evaluation ◽

10.29221/jce.1999.2.1.231 ◽

1999 ◽

Vol 2 (1) ◽

pp. 231-249

Author(s):

Myung-hee Yun ◽

Seong-bin Kang

Keyword(s):

Sampling Methods ◽

Aptitude Tests ◽

Line Sampling ◽

Item Parameters ◽

On Line

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Designing a CRM Framework for online & offline sports footwear retailers

"Carácter" Revista Cientifica de la Universidad Del Pacifico ISSN 2602-8476 ◽

10.35936/caracter.v3i1.41 ◽

2015 ◽

Vol 3 (1) ◽

Author(s):

Rodrigo Cueva ◽

Guillem Rufian ◽

Maria Gabriela Valdes

Keyword(s):

Environmental Changes ◽

Customer Relationship ◽

Business Strategies ◽

Economic Sectors ◽

Running Shoes ◽

Brick And Mortar ◽

Key Factor ◽

On Line ◽

The Impact

The use of Customer Relationship Managers to foster customers loyalty has become one of the most common business strategies in the past years. However, CRM solutions do not fill the abundance of happily ever-after relationships that business needs, and each client’s perception is different in the buying process. Therefore, the experience must be precise, in order to extend the loyalty period of a customer as much as possible. One of the economic sectors in which CRM’s have improved this experience is retailing, where the personalized attention to the customer is a key factor. However, brick and mortar experiences are not enough to be aware in how environmental changes could affect the industry trends in the long term. A base unified theoretical framework must be taken into consideration, in order to develop an adaptable model for constructing or implementing CRMs into companies. Thanks to this approximation, the information is complemented, and the outcome will increment the quality in any Marketing/Sales initiative. The goal of this article is to explore the different factors grouped by three main domains within the impact of service quality, from a consumer’s perspective, in both on-line and off-line retailing sector. Secondly, we plan to go a step further and extract base guidelines about previous analysis for designing CRM’s solutions focused on the loyalty of the customers for a specific retailing sector and its product: Sports Running Shoes.

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The suitability and application of a GFP-actin fusion protein for long-term imaging of the organization and dynamics of the cytoskeleton in mammalian cells

European Journal of Cell Biology ◽

10.1016/s0171-9335(98)80075-7 ◽

1998 ◽

Vol 77 (2) ◽

pp. 81-90 ◽

Cited By ~ 63

Author(s):

Axel Choidas ◽

Andreas Jungbluth ◽

Antonio Sechi ◽

John Murphy ◽

Axel Ullrich ◽

...

Keyword(s):

Fusion Protein ◽

Mammalian Cells

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Water vapor total column measurements using the Elodie Archive at Observatoire de Haute Provence from 1994 to 2004

Atmospheric Measurement Techniques Discussions ◽

10.5194/amtd-2-1075-2009 ◽

2009 ◽

Vol 2 (2) ◽

pp. 1075-1097

Author(s):

A. Sarkissian ◽

J. Slusser

Keyword(s):

Water Vapor ◽

Water Vapor Absorption ◽

The Earth ◽

Vapor Absorption ◽

On Line ◽

High Resolution Spectrometer ◽

Long Term Trend ◽

Resolution Spectrometer ◽

Total Column

Abstract. Water vapor total column measurements at Observatoire de Haute Provence (5°42' E, +43°55' N), south of France, were obtained using observations of astronomical objects made between July 1994 and December 2004 on the 193-cm telescope with the high-resolution spectrometer Elodie. Spectra of stars, nebulae, and other astronomical objects were taken regularly during 10 years. More than 18 000 spectra from 400 nm to 680 nm are available on-line in the Elodie Archive. This archive, usually explored by astronomers, contains information to study the atmosphere of the Earth. Water vapor absorption lines appear in the visible in delimited bands that astronomers often avoid for their spectral analysis. We used the Elodie Archive with two objectives: firstly, to retrieve seasonal variability and long-term trend of atmospheric water vapor, and secondly, to remove signatures in spectra for further astronomical or geophysical use. The tools presented here are developed following, when possible, formats and standards recommended by the International Virtual Observatory Alliance.

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Pollution Loads Discharged from Combined Sewer Overflows: Theoretical Approach and Long Term Numerical Assessment

Hydrology Research ◽

10.2166/nh.1992.0003 ◽

1992 ◽

Vol 23 (1) ◽

pp. 27-48 ◽

Cited By ~ 2

Author(s):

Paolo Mignosa ◽

Alessandro Paoletti

Keyword(s):

Theoretical Approach ◽

Rainfall Regime ◽

Combined Sewer Overflows ◽

Event Simulation ◽

Numerical Assessment ◽

Combined Sewer ◽

On Line ◽

Sewer Overflows ◽

Two Parameters

The paper describes a theoretical analysis and a numerical assessment of pollutant loads discharged from Combined Sewer Overflows (CSOs) - with or without stormwater tanks – into the environment. The theoretical approach was based on certain simple assumptions, reasonably valid if the time scale of the problem involved is long enough (month/ year), in that single-event simulation is not interesting at all. Two main parameters related to the rainfall regime were found to be significant: the total volume of water discharged from the structure and the effective mixing factor between sanitary sewage and storm runoff. A numerical assessment of these two parameters was then made, on an annual basis, by means of a long-term rainfall series recorded in Milan, Italy. Both the “simple” CSO structure and the CSO coupled with stormwater tanks (on-line or off-line) were considered. The resulting graphs make it possible to evaluate the total annual load discharged from CSOs into the environment and the potential reduction obtained by adding a storage capacity to the overflow. This estimation could be of interest for persistent pollutants (phosphorus, heavy metals) discharged into low-recirculation bodies (lakes, estuaries, lagoons, closed seas).

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27 On-line national survey to explore the changes in practice of paediatric long-term ventilation

10.1136/bmjresp-2021-bssconf.24 ◽

2021 ◽

Author(s):

Heather Elphick ◽

Nicki Barker ◽

Aditi Singh ◽

Catherine Jesson ◽

Omendra Narayan

Keyword(s):

National Survey ◽

On Line ◽

Long Term Ventilation

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Practical Remote Workflow Solutions for Complex Digital Projects: Opportunities in a Pandemic

Collections ◽

10.1177/15501906211052720 ◽

2021 ◽

pp. 155019062110527

Author(s):

J.A. Pryse

Keyword(s):

Large Scale ◽

The Political ◽

Workflow Model ◽

Practical Application ◽

Pilot Phase ◽

Digital Videos ◽

On Line ◽

Systematic Solution ◽

Long Term Preservation

The spread of COVID-19 has created numerous challenges in the field of archive management. Limited in-house office space, furloughs of personnel, and inconsistency, has highlighted the potential for the Carl Albert Congressional Research and Studies Center Archives (Center) to develop and implement improved accessibility measures to thousands of linear feet of material. Addition ally, the Center has found unique opportunities to collaborate with multiple academic institutions to propose large-scale digitization program exhibitions using the Center’s remote workflow model. One of the largest, most complex collections the Center has worked with during this time is the Political Commercial Collection (the Collection), which holds 119,000 film, audio, and videotape recordings of commercials aired between 1936 and present. It is the largest collection of political commercials in the world. The Center has developed a working pilot digitization project that has currently resulted in access to 16,000 digital videos for public researchers and over 10,000 available for on-line streaming during the pilot phase between April 16, 2020, and December 1, 2020. This paper presents the practical application of the Center’s simplified “Linear Reciprocity Workflow Model” to provide a systematic solution for digital and long-term preservation of complex collections. The Center has proven that limited personnel and reduced resources need not interrupt continued access to archival repositories.

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