scholarly journals The enrichment of chlorogenic acid from Eucommia ulmoides leaves extract by mesoporous carbons

2018 ◽  
Vol 1087-1088 ◽  
pp. 6-13 ◽  
Author(s):  
Guotong Qin ◽  
Jing Ma ◽  
Wei Wei ◽  
Jaja Li ◽  
Fangqing Yue
2021 ◽  
Vol 16 (10) ◽  
pp. 1934578X2110461
Author(s):  
Hua Jiang ◽  
Jun Li ◽  
Ning Zhang ◽  
Hai-Yang He ◽  
Jia-Min An ◽  
...  

Chlorogenic acid has been proved to have cardiovascular protection, antibacterial, antiviral, hemostatic, and hypolipidemia effects. Modern scientific research on the bioactivity of chlorogenic acid has been extended to the fields of food, medicine, health care and daily-use chemical industry. The aim of this research was to optimize the extraction conditions for chlorogenic acid from Eucommia ulmoides (Eucommiaceae) leaves. The significant variables were screened and optimized by a combination of Plackett-Burman test and Box-Behnken design. Optimum extraction parameters with ethanol concentration of 50%, solvent pH value of 3, and particle size of 60 mesh were determined according to variance analysis and contour plots. Under these conditions, the yield of chlorogenic acid was up to 4.36 mg/g, which was basically consistent with the theoretical prediction value of 4.50 mg/g. This study also proved the potential antioxidant activity of E. ulmoides leaves. The optimal extract of E. ulmoides leaves rich in chlorogenic acid showed the highest antioxidant activity in the FRAP method, which was 219.8 μM Trolox equivalents (TE) per g extract weight (EW) (μM TE/g EW). The DPPH method gave a similar value (168 μM TE/g EW) to the ABTS method (152 μM TE/g EW). The established extraction process was efficient in the recovery of chlorogenic acid from E. ulmoides leaves, encouraging its valorization as a cheap and sustainable alternative for the isolation of chlorogenic acid.


2021 ◽  
Vol 54 (8) ◽  
pp. 571-580
Author(s):  
Mayowa Oyebanji ◽  
Xuan Dong ◽  
Qianqian Zhang ◽  
Guohua Yao ◽  
Qing Huang

2010 ◽  
Vol 37 (5) ◽  
pp. 447-454 ◽  
Author(s):  
Xiaomin Chen ◽  
Xiaoxue Sang ◽  
Shaohe Li ◽  
Shujun Zhang ◽  
Linhan Bai

2012 ◽  
Vol 550-553 ◽  
pp. 1270-1273 ◽  
Author(s):  
Hua Shang ◽  
Di Liu ◽  
Hui Jun Ji

The flavonoids and chlorogenic acid were separated and purified from Eulmoids ulmoids leaves, and their antioxidation activity in vivo and in vitro was investigated by animal test and free radical scavenging test. The result shows that Eucommia ulmoides leaves flavones and chlorogenic acid can significantly reduce the content of malondialchehyche (MDA) (p<0.05), but only Eucommia ulmoides leaves chlorogenic acid can significantly enhance the activity of superoxide dismutase (SOD) (p<0.05) and glutathione peroxidase (GSH-PX) (p<0.05), which means that Eucommia ulmoides leaves chlorogenic acid has more significant antioxidation activity in vivo; both Eucommia ulmoides Leaves flavones and chlorogenic acid have good capacity on scavenging hydroxyl radical (HO•), superoxide anion radical (O2-•) and 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH•), and show good doses-response relationship, but Eucommia ulmoides leaves chlorogenic acid shows highest scavenging rate on every radical, which means that Eucommia ulmoides leaves chlorogenic acid has more significant antioxidation activity in vitro.


2009 ◽  
Vol 61 (4) ◽  
pp. 811-817 ◽  
Author(s):  
Jianbo Xiao ◽  
Xinlin Wei ◽  
Yuanfeng Wang

A reversed-phase liquid chromatographic method was developed for the quantitative determination of three major hypotensive compounds, namely geniposidic acid, chlorogenic acid, and geniposide in the bark of Eucommia ulmoides. Soxhlet extraction of GPA, GPS, and CA from E. ulmoides was optimized according to the Taguchi experimental design. Maximum global yields were obtained using the following conditions: extraction temperature, 80?C; extraction time, 1 h; number of extractions, three; solvent volume, 16 ml/g of sample; and 50% ethanol concentration in water. Optimal conditions of separation and detection were achieved on a Diamonsil ODS C18 column (150 mm ? 4.6 mm, 5 ?m) with a linear gradient of methanol and 0.04% aqueous phosphoric acid (v/v) at a flow rate of 1.0 ml/min and detection wavelength of 240 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 3% for intra- and inter-day assays, and the recovery percentage of the method was 95-104%, with a relative standard deviation (R.S.D.) of less than 5%. The current assay method was used for quantitative determination of geniposidic acid, chlorogenic acid, and geniposide in five samples of E. ulmoides with different age. The results indicate that the developed method could be readily utilized as a quality control method in working with E. ulmoides.


2021 ◽  
Author(s):  
Changjiang Qian ◽  
Rongrong Zhang ◽  
Jia Li ◽  
Zongsheng Huang ◽  
Xun Liu ◽  
...  

Abstract To find out the genuine characteristics of Eucommia ulmoides produced in Guizhou.The habitat, functional characters and the content of medicinal components of Eucommia ulmoides in Guizhou were studied by using the method of sample survey combined with typical survey, related laboratory experiments and quantitative analysis. The results showed that the yield of Eucommia ulmoides plantation in Guizhou was divided into low altitude, low middle and high temperature rain slope latitude mixing, short sunshine type(A type), medium altitude, low longitude and latitude, high temperature rain, positive oblique steep slope, medium sunshine type (B type), Middle altitude, low longitude and latitude, moderate high temperature rain, Shady side and Sunny side have gentle deflection steep slope, medium sunshine type (C type), High altitude, low longitude and latitude, low temperature moderate rain, positive gentle slope, long sunshine type (D type); Different types of Eucommia ulmoides plantation, Different habitat quality, B type is intensity karst rocky desertification habitat, A type is potential karst rocky desertification habitat,The C and D types are light and moderate rocky desertification areas respectively, The species diversity of shrub layer in Eucommia ulmoides plantation was higher in D type and B type, A type and C type followed; There was no significant difference in root carbon content and leaf nitrogen content in 4 types of Eucommia ulmoides plantation, Among the four types of A、B、C、D, there were significant or extremely significant differences in other indexes of plant functional traits; Both genipinic acid and aucubin had the highest content of root bark, followed by trunk bark and lowest leaves, Chlorogenic acid is the opposite, The content of geniposide was higher in trunk bark and lower in root bark and leaves; Genipinic acid is higher in D type, Aucubin is higher in A and D type, Chlorogenic acid has higher leaves content in B type, Geniposide was the highest in trunk bark of D type; The element enrichment coefficient K and Mn leaves are the largest, the largest in trunk bark is Ca and Zn, Fe root bark is the largest; Effects of soil potassium, phosphorus, pH value and bulk weight on the functional traits of Eucommia ulmoides were significant. The contents of medicinal components in root bark, trunk bark and leaves was influenced by species diversity of shrub layer, The contents of geniposide in root bark, aucubin in root bark and trunk bark, genipinic acid in bark and chlorogenic acid in leaves were particularly affected by soil physical and chemical indexes and metal element contents, The functional traits of Eucommia ulmoides can affect the content of medicinal components in root bark, trunk bark and leaves, Especially on the root bark, trunk bark and leaves in the content of aucubin content; The content of medicinal components of Eucommia ulmoides was high and stable. The above research results have important theoretical reference significance for the cultivation of Eucommia ulmoides and the cultivation of target medicinal components and the comprehensive exploitation and utilization of resources.


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