The Antioxidation Activity In Vivo and In Vitro of Flavonoids and Chlorogenic Acid Isolated from Eucommia ulmoides Leaves

2012 ◽  
Vol 550-553 ◽  
pp. 1270-1273 ◽  
Author(s):  
Hua Shang ◽  
Di Liu ◽  
Hui Jun Ji
Keyword(s):  
Chlorogenic Acid ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Eucommia Ulmoides ◽  
Response Relationship ◽  
Antioxidation Activity

The flavonoids and chlorogenic acid were separated and purified from Eulmoids ulmoids leaves, and their antioxidation activity in vivo and in vitro was investigated by animal test and free radical scavenging test. The result shows that Eucommia ulmoides leaves flavones and chlorogenic acid can significantly reduce the content of malondialchehyche (MDA) (p<0.05), but only Eucommia ulmoides leaves chlorogenic acid can significantly enhance the activity of superoxide dismutase (SOD) (p<0.05) and glutathione peroxidase (GSH-PX) (p<0.05), which means that Eucommia ulmoides leaves chlorogenic acid has more significant antioxidation activity in vivo; both Eucommia ulmoides Leaves flavones and chlorogenic acid have good capacity on scavenging hydroxyl radical (HO•), superoxide anion radical (O2-•) and 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH•), and show good doses-response relationship, but Eucommia ulmoides leaves chlorogenic acid shows highest scavenging rate on every radical, which means that Eucommia ulmoides leaves chlorogenic acid has more significant antioxidation activity in vitro.

scholarly journals Exploration of Antioxidant and Anticancer Activity of Stephania japonica Leaves Extract

2021 ◽  
pp. 256-272
Author(s):  
Md. Dobirul Islam ◽  
Ariful Islam ◽  
Naoshia Tasnin ◽  
Syeda Farida Akter ◽  
Md. Salim Uddin
Keyword(s):  
Free Radical ◽  
Cell Growth ◽  
Anticancer Activity ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cell Growth Inhibition

Aims: The demand for antioxidants from the natural source has drawn promising attention to outturn desired pharmacological effect by subsidizing the adverse effect for treating cancer. This study evaluated the antioxidant activity of Stephania japonica leaves extracts to explore the anticancer activity.    Methods: Antioxidant potential of crude extracts were evaluated using various methods which include total antioxidant activity, ferric reducing antioxidant assay, DPPH free radical, ABTS free radical, nitric oxide and superoxide anion radical scavenging assay. Anticancer activity was determined in vitro by MTT assay and in vivo on mice against Ehrlich ascites carcinoma (EAC) cell. Results: Phytoconstituents with free radical scavenging capacity were quantified in terms of inhibitory concentration (IC50) with the values of 17.00±3.22 µg/mL, 33.30 ± 5.45 µg/mL, 43.70±5.26 µg/mL and 52.30±1.07 µg/mL in DPPH, ABTS, superoxide anion radical and nitric oxide free radical scavenging assay, respectively as the highest quencher, acetone extract of S. japonica leaves (ASJL). ASJL and methanol extract (MSJL) showed low lethal dose (LD50) values of 21.76 and 26.63, respectively indicating higher toxicity.  In vitro anti-proliferative activity (MTT assay), ASJL and MSJL were exhibited 15.44±2.96 to 80.94±2.87 and 11.76±3.74 to 74.25±1.49 percent of cell growth inhibition, respectively at the concentration of 10.28 µg/mL to 833.33 µg/mL. In in vivo test, ASJL and MSJL at the dose of 100 mg/kg/day and 200 mg/kg/day (i.p.) showed cell growth inhibition of 58.25±4.24% to 79.09±2.45% and 46.26±2.68% to 61.74±4.41%, respectively on EAC cell tumor-bearing mice. The life span of intraperitoneal induced EAC cell bearing mice was increased to 29.05% and 57.02% on the treatment of ASJL with 100 and 200 mg/kg/day, respectively. Conclusions: The free radical scavenger of S. japonica leaves extract was stimulated the host immunity and inhibited the EAC cell growth through initiating the apoptosis cell death program. Therefore, S. japonica leaves might be utilized as a potent anticancer natural source.

scholarly journals Antioxidant and radical scavenging activities of some norcantharidin and bridged perhydroisoindole derivatives

2013 ◽  
Vol 78 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Aysegul Peksel ◽  
Cumali Celik ◽  
Nuket Ocal ◽  
Refiye Yanardag
Keyword(s):  
Free Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Antioxidant Power

A series of norcantharidin and bridged perhydroisoindole derivatives were evaluated for their antioxidant and radical scavenging activities. Different in vitro methodologies such as total reducing power, 1,1-diphenyl-2- picrylhydrazil (DPPH?) free radical scavenging, superoxide anion radical scavenging and metal chelating activities were used. Among 11 compounds tested, 7 compounds showed potent reducing power activity and 8 compounds showed potent superoxide anion radical scavenging activity. All the tested compounds exhibited potent free radical scavenging ability. The results showed that the synthesized compounds had effective antioxidant power.

scholarly journals In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 309
Author(s):  
Olukayode O. Aremu ◽  
Adebola O. Oyedeji ◽  
Opeoluwa O. Oyedeji ◽  
Benedicta N. Nkeh-Chungag ◽  
Constance R. Sewani Rusike
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Methyl Ester ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypertensive Rats ◽  
Total Antioxidant

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

Evaluation of antioxidant activity of Melothria maderaspatana in vitro

2010 ◽  
Vol 5 (2) ◽  
pp. 224-230 ◽  
Author(s):  
Boobalan Raja ◽  
Kodukkur Pugalendi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Melothria Maderaspatana

AbstractIn this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentrations of leaf extract exhibited free radical scavenging and antioxidant power, and the preventive effects were in a dose-dependent manner. The antioxidant activities of the above were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and α-tocopherol. The results obtained in the present study indicate that the M. maderaspatana extract could be considered a potential source of natural antioxidant.

In vitro free radical scavenging and in vivo antioxidant potential of mulberry (Morus indica L.) leaves

2014 ◽  
Vol 4 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Bondada Andallu ◽  
Mahalakshmi Shankaran ◽  
Rajeshwari Ullagaddi ◽  
Shobha Iyer
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antioxidant Potential

scholarly journals In vitro and in vivo evaluation of pharmacological potentials of Campsis radicans L

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Mirazul Islam ◽  
Tabassum Jannat ◽  
Md. Ruhul Kuddus ◽  
Mohammad Abdur Rashid ◽  
Mohammad Rashedul Haque
Keyword(s):  
Castor Oil ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Thrombolytic Activity ◽  
Tail Immersion ◽  
Soluble Fractions

Abstract Background Campsis radicans L. is a flowering plant in Bangladesh, traditionally used for the treatment of several human diseases. In this study, in vitro antioxidant, thrombolytic, membrane stabilizing and in vivo analgesic, hypoglycemic, anti-diarrheal and CNS antidepressant activities of organic soluble fractions of crude methanol extract of C. radicans leaf were investigated using appropriate experimental models. Methods The leaves of C. radicans were collected, authenticated, dried and extracted with methanol at room temperature for 30 days. The concentrated methanol extract was partitioned to petroleum-ether (PESF), dichloromethane (DMSF) and ethyl acetate (EASF) soluble fractions. The antioxidant activity of these fractions was determined by DPPH free radical scavenging method. Total phenolic content was determined by the Folin-Ciocalteau’s spectrophotometric method. The thrombolytic activity was assessed by measuring clot lysis ability whereas the membrane stabilizing activity was evaluated by heat- and hypotonic solution-induced hemolysis assay. Tail immersion procedure and acetic acid- induced writhing model were used to measure the analgesic activity of C. radicans. The hypoglycemic, anti-diarrheal and CNS antidepressant activities were determined by oral glucose tolerance test, castor oil-induced diarrheal model and thiopental-sodium induced sleeping time test in mice, respectively. Results All the organic soluble fractions of C. radicans contained phenolic compounds varying from 6.38 to 60.13 mg of GAE/gm of extractive, while in DPPH assay, EASF showed the highest free radical scavenging activity with IC50 is 4.69 μg/ml. The PESF exhibited highest thrombolytic activity (57.14% clot lysis) and the DMSF showed maximum 53.95% inhibition of heat-induced hemolysis of human RBCs. In both tail immersion and acetic acid induced writhing models, the PESF, DMSF, EASF at the doses of 200 and 400 mg/kg body weight, induced a significant (P < 0.001) decrease in the painful sensation in mice. Substantial (P < 0.05) anti-hyperglycemic activity of test samples was found in mice loaded with glucose at the same doses mentioned earlier. Castor oil induced diarrheal test of the plant extractives has shown significant effect in comparison to control group. In CNS antidepressant activity assay, the test samples were able to reduce the duration of sleep in mice caused by thiopental administration. Conclusion All these findings revealed that C. radicans possess significant antioxidant, thrombolytic, membrane stabilizing, analgesic, hypoglycemic, anti-diarrheal and CNS antidepressant activities.

scholarly journals In vitro and in vivo assessment of free radical scavenging and antioxidant activities of Veronica persica Poir

2018 ◽  
Vol 64 (8) ◽  
pp. 57 ◽  
Author(s):  
Javad Sharifi-Rad ◽  
Mehdi Sharifi-Rad ◽  
Bahare Salehi ◽  
Marcello Iriti ◽  
Amir Roointan ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vivo Assessment

scholarly journals Phytochemical Profile, Free Radical Scavenging and Anti-Inflammatory Properties of Acalypha Indica Root Extract: Evidence from In Vitro and In Vivo Studies

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6251
Author(s):  
Ravi Sahukari ◽  
Jyothi Punabaka ◽  
Shanmugam Bhasha ◽  
Venkata Subbaiah Ganjikunta ◽  
Shanmugam Kondeti Ramudu ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vivo Studies ◽  
Root Extract ◽  
Dependent Manner ◽  
Anti Inflammatory ◽  
Acalypha Indica

In our in vitro and in vivo studies, we used Acalypha indica root methanolic extract (AIRME), and investigated their free radical scavenging/antioxidant and anti-inflammatory properties. Primarily, phytochemical analysis showed rich content of phenols (70.92 mg of gallic acid/g) and flavonoids (16.01 mg of rutin/g) in AIRME. We then performed HR-LC-MS and GC-MS analyses, and identified 101 and 14 phytochemical compounds, respectively. Among them, ramipril glucuronide (1.563%), antimycin A (1.324%), swietenine (1.134%), quinone (1.152%), oxprenolol (1.118%), choline (0.847%), bumetanide (0.847%) and fenofibrate (0.711%) are the predominant phytomolecules. Evidence from in vitro studies revealed that AIRME scavenges DPPH and hydroxyl radicals in a concentration dependent manner (10–50 μg/mL). Similarly, hydrogen peroxide and lipid peroxidation were also remarkably inhibited by AIRME as concentration increases (20–100 μg/mL). In vitro antioxidant activity of AIRME was comparable to ascorbic acid treatment. For in vivo studies, carrageenan (1%, sub-plantar) was injected to rats to induce localized inflammation. Acute inflammation was represented by paw-edema, and significantly elevated (p < 0.05) WBC, platelets and C-reactive protein (CRP). However, AIRME pretreatment (150/300 mg/kg bodyweight) significantly (p < 0.05) decreased edema volume. This was accompanied by a significant (p < 0.05) reduction of WBC, platelets and CRP with both doses of AIRME. The decreased activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase in paw tissue were restored (p < 0.05 / p < 0.01) with AIRME in a dose-dependent manner. Furthermore, AIRME attenuated carrageenan-induced neutrophil infiltrations and vascular dilation in paw tissue. For the first time, our findings demonstrated the potent antioxidant and anti-inflammatory properties of AIRME, which could be considered to develop novel anti-inflammatory drugs.

Sign in / Sign up

Export Citation Format

Share Document