Targeting gene expression during the early bone healing period in the mandible: A base for bone tissue engineering

2015 ◽  
Vol 43 (8) ◽  
pp. 1452-1460 ◽  
Author(s):  
Benedicta E. Beck-Broichsitter ◽  
Anneke N. Werk ◽  
Ralf Smeets ◽  
Alexander Gröbe ◽  
Max Heiland ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Healing Period

scholarly journals 3D-Bioprinting Strategies Based on In Situ Bone-Healing Mechanism for Vascularized Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 287
Author(s):  
Ye Lin Park ◽  
Kiwon Park ◽  
Jae Min Cha
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Critical Size ◽  
Current Knowledge ◽  
3D Bioprinting ◽  
The Past ◽  
Regeneration Processes

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

scholarly journals Effects of bone tissue engineering triad components on vascularization process: comparative gene expression and histological evaluation in an ectopic bone-forming model

2016 ◽  
Vol 30 (6) ◽  
pp. 1122-1131 ◽  
Author(s):  
Jelena G. Najdanović ◽  
Vladimir J. Cvetković ◽  
Sanja Stojanović ◽  
Marija Đ. Vukelić-Nikolić ◽  
Maja M. Čakić-Milošević ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Histological Evaluation ◽  
Ectopic Bone

Combination of bone tissue engineering and BMP-2 gene transfection promotes bone healing in osteoporotic rats

2008 ◽  
Vol 32 (9) ◽  
pp. 1150-1157 ◽  
Author(s):  
Youchao Tang ◽  
Wei Tang ◽  
Yunfeng Lin ◽  
Jie Long ◽  
Hang Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Gene Transfection

scholarly journals A review of biomaterials in bone defect healing, remaining shortcomings and future opportunities for bone tissue engineering

2018 ◽  
Vol 7 (3) ◽  
pp. 232-243 ◽  
Author(s):  
T. Winkler ◽  
F. A. Sass ◽  
G. N. Duda ◽  
K. Schmidt-Bleek
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Defect ◽  
Bone Healing ◽  
Lessons Learned ◽  
Form And Function ◽  
Defect Healing ◽  
Bone Defect Healing

Despite its intrinsic ability to regenerate form and function after injury, bone tissue can be challenged by a multitude of pathological conditions. While innovative approaches have helped to unravel the cascades of bone healing, this knowledge has so far not improved the clinical outcomes of bone defect treatment. Recent findings have allowed us to gain in-depth knowledge about the physiological conditions and biological principles of bone regeneration. Now it is time to transfer the lessons learned from bone healing to the challenging scenarios in defects and employ innovative technologies to enable biomaterial-based strategies for bone defect healing. This review aims to provide an overview on endogenous cascades of bone material formation and how these are transferred to new perspectives in biomaterial-driven approaches in bone regeneration. Cite this article: T. Winkler, F. A. Sass, G. N. Duda, K. Schmidt-Bleek. A review of biomaterials in bone defect healing, remaining shortcomings and future opportunities for bone tissue engineering: The unsolved challenge. Bone Joint Res 2018;7:232–243. DOI: 10.1302/2046-3758.73.BJR-2017-0270.R1.

scholarly journals Fibrin as a Multipurpose Physiological Platform for Bone Tissue Engineering and Targeted Delivery of Bioactive Compounds

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 556 ◽  
Author(s):  
Bruno Bujoli ◽  
Jean-Claude Scimeca ◽  
Elise Verron
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Targeted Delivery ◽  
Blood Clot ◽  
Critical Role ◽  
Natural Polymers ◽  
Gold Standard Method ◽  
Bioactive Agents

Although bone graft is still considered as the gold standard method, bone tissue engineering offers promising alternatives designed to mimic the extracellular matrix (ECM) and to guide bone regeneration process. In this attempt, due to their similarity to the ECM and their low toxicity/immunogenicity properties, growing attention is paid to natural polymers. In particular, considering the early critical role of fracture hematoma for bone healing, fibrin, which constitutes blood clot, is a candidate of choice. Indeed, in addition to its physiological roles in bone healing cascade, fibrin biochemical characteristics make it suitable to be used as a multipurpose platform for bioactive agents’ delivery. Thus, taking advantage of these key assets, researchers and clinicians have the opportunity to develop composite systems that might further improve bone tissue reconstruction, and more generally prevent/treat skeletal disorders.

Mechanical Stimulation Mediates Gene Expression in MC3T3 Osteoblastic Cells Differently in 2D and 3D Environments

2010 ◽  
Vol 132 (4) ◽  
Author(s):  
Matthew J. Barron ◽  
Chung-Jui Tsai ◽  
Seth W. Donahue
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Fluid Flow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
3D Culture ◽  
Osteoblastic Cells ◽  
3D Cultures ◽  
Static Conditions ◽  
2D And 3D

Successful bone tissue engineering requires the understanding of cellular activity in three-dimensional (3D) architectures and how it compares to two-dimensional (2D) architecture. We developed a perfusion culture system that utilizes fluid flow to mechanically load a cell-seeded 3D scaffold. This study compared the gene expression of osteoblastic cells in 2D and 3D cultures, and the effects of mechanical loading on gene expression in 2D and 3D cultures. MC3T3-E1 osteoblastlike cells were seeded onto 2D glass slides and 3D calcium phosphate scaffolds and cultured statically or mechanically loaded with fluid flow. Gene expression of OPN and FGF-2 was upregulated at 24 h and 48 h in 3D compared with 2D static cultures, while collagen 1 gene expression was downregulated. In addition, while flow increased OPN in 2D culture at 48 h, it decreased both OPN and FGF-2 in 3D culture. In conclusion, gene expression is different between 2D and 3D osteoblast cultures under static conditions. Additionally, osteoblasts respond to shear stress differently in 2D and 3D cultures. Our results highlight the importance of 3D mechanotransduction studies for bone tissue engineering applications.

scholarly journals Design, Materials, and Mechanobiology of Biodegradable Scaffolds for Bone Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-21 ◽  
Author(s):  
Marco A. Velasco ◽  
Carlos A. Narváez-Tovar ◽  
Diego A. Garzón-Alvarado
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Computational Models ◽  
Manufacturing Processes ◽  
Scaffold Design ◽  
Biodegradable Scaffolds ◽  
Design Materials

A review about design, manufacture, and mechanobiology of biodegradable scaffolds for bone tissue engineering is given. First, fundamental aspects about bone tissue engineering and considerations related to scaffold design are established. Second, issues related to scaffold biomaterials and manufacturing processes are discussed. Finally, mechanobiology of bone tissue and computational models developed for simulating how bone healing occurs inside a scaffold are described.

Born Again Bone: Tissue Engineering for Bone Repair

Physiology ◽  
2001 ◽  
Vol 16 (5) ◽  
pp. 208-213 ◽  
Author(s):  
Martin Braddock ◽  
Parul Houston ◽  
Callum Campbell ◽  
Patrick Ashcroft
Keyword(s):  
Tissue Engineering ◽  
Growth Factor ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Bone Repair ◽  
Traumatic Injury ◽  
Gene Therapies ◽  
Born Again

Destruction of bone tissue due to disease and inefficient bone healing after traumatic injury may be addressed by tissue engineering techniques. Growth factor, cytokine protein, and gene therapies will be developed, which, in conjunction with suitable carriers, will regenerate missing bone or help in cases of defective healing.

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