Short communication: Finetuning molecular markers for efficient selection of vitamin A-rich tropical maize lines in a molecular breeding scheme

2021 ◽  
Vol 97 ◽  
pp. 103149
Author(s):  
Deborah Babalola ◽  
Abebe Menkir ◽  
Oluyinka Ilesanmi ◽  
Queen Obi ◽  
Melaku Gedil
Bragantia ◽  
2014 ◽  
Vol 73 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Ítalo Stefanine Correia Granato ◽  
Felipe Pereira Bermudez ◽  
Gabriel Gonçalves dos Reis ◽  
Julio César Dovale ◽  
Glauco Vieira Miranda ◽  
...  

Nitrogen (N) limitation in maize crops is related to the fact that the efficiency of nitrogen fertilization in maize does not exceed 50%, primarily due to volatilization, denitrification and soil leaching. Therefore, the development of new nitrogen use efficient (NUE) cultivars is necessary. The aim of the present study was to develop indices for the accurate selection of NUE maize genotypes for use in conditions of both high and low N availability. The experiment was conducted in a greenhouse (20º45'14"S; 42º52'53"W) at the Federal University of Viçosa during October 2010. A total of 39 experimental hybrid combinations and 14 maize lines differing in NUE were evaluated under two N availability conditions. We determined the relative importance of the studied characters using principal component analysis, factor analysis and by developing efficient selection indices. We conclude that indirect and early selection of tropical maize genotypes can be performed using the indices I HN = 0.022 SDM + 0.35 RSDM + 0.35 RL A + 0.35 NUE for high N availability environments and I LN = -0.06 RSDM + 0.35 RSA A + 0.35 RL A + 0.39 SDM for low N availability environments.


2021 ◽  
Vol 32 (Issue 2) ◽  
pp. 24-30
Author(s):  
Guindón M.F. ◽  
F. Cazzola ◽  
C.J. Bermejo ◽  
M.A. Espósito ◽  
I. Gatti ◽  
...  

Conventional breeding can be complemented by different strategies that increase the efficiency of the methodologies and the current rate of increase in yields in order to meet demand. The use of molecular markers with the aim of developing linkage maps of the species, the use of Blup (Best Linear Unbiased Prediction) for an efficient selection of progenitors to hybridize, the use of in vitro culture to artificially increase the number of F1 plants or the use of digital phenotyping for efficient digital characterization that can be performed during the periodic and routine regeneration of accessions in germplasm collections. Key words: Molecular markers, Blup, in vitro culture, digital phenotyping.


2016 ◽  
Vol 9 (1) ◽  
pp. 80-95
Author(s):  
Agus Sudibyo ◽  
Sardjono Sardjono

Crude palm oil (CPO)is the richest natural plant source of carotenoids in terms of retinol (pro-vitamin A) equivalent, whereas palm oil mill effluent (POME) is generated from palm oil industry that contains oil and carotenes that used to be treated before discharge. Carotenoids are importance in animals and humans for the purpose of the enhancement of immune response, conversion of vitamin A and scavenging of oxygen radicals. This component has different nutritional  functions and benefits to humaan health. The growing interest in the other natural sources of beta-carotene and growing awareness to prevent pollution has stimulated the industrial use of CPO and POME as a raw material for carotenoids extraction. Various technologies of extraction and separation have been developed in order to recover of carotenoids.This article reports on various technologies that have been developed in order to recover of carotenoids from being destroyed in commercial refining of palm oil and effects of some various treatments on the extraction end separation for carotenoid from palm oil and carotenoids concentration. Principally, there are different technologies, and there is one some future which is the use of solvent. Solvent plays important role  in the most technologiest, however the problem of solvents which are used is that they posses potentiaal fire health and environmental hazards. Hence selection of the  most safe, environmentally friendly and cost effective solvent is important to design of alternative extraction methods.Chemical molecular product design is one of the methods that are becoming more popular nowadays for finding solvent with the desired properties prior to experimental testing.ABSTRAKMinyak sawit kasar merupakan sumber karotenoid terkaya yang berasal dari tanaman sawit sebagai senyawa yang sama dengan retinol atau pro-vitamin A; sedangkan limbah pengolahan minyak sawit dihasilkan dari industri pengolahan minyak sawit yang berisi minyak dan karotene yang perlu diberi perlakuan terlebih dahulu sebelum dibuang. Karotenoid merupakan bahan penting yang diperlukan pada hewan dan manusia guna memperkuat tanggapan terhadap kekebalan, konversi ke vitamin A dan penangkapan gugus oksigen radikal. Dengan berkembangnya ketertarikan dalam mencari beta-karotene yang bersumber dari alam lain dan meningkatnya kesadaran untuk mencegah adanya pencemaran lingkungan, maka mendorong suatu industri untuk menggunakan CPO dan POME sebagai bahan baku untuk diekstrak karotenoidnya. Berbagai macam teknologi guna mengekstrak dan memisahkan karotenoid telah dikembangkan untuk mendapatkan kembali karotenoidnya. Makalah ini melaporkan dan membahas berbagai jenis teknologi yang telah dikembangkan guna mendapatkan kembali senyawa karotenoid dari kerusakan di dalam proses pemurnian minyak sawit secara komersial dan pengaruh beberapa perlakuan terhadap ekstrasi dan pemisahan karotenoid dari minyak sawit dan konsentrasi karotenoidnya. Pada prinsipnya, berbagai teknologi yang digunakan untuk mengekstrak dan memisahkan karotenoid terdapat perbedaan, dan terdapat salah satu teknologi yang digunakan untuk esktrasi dan pemisahan karotenoid adalah menggunakan bahan pelarut. Pelarut yang digunakan mempunyai peranan yang penting dalam teknologi ekstrasi; namun pelarut yang digunakan untuk mengekstrak tersebut mempunyai persoalan karena berpotensi mengganggu kesehatan dan membahayakan cemaran lingkungan. Oleh karena itu, pemilihan jenis teknologi yang aman, ramah terhadap lingkungan dan biaya yang efektif untuk penggunaan pelarut merupakan hal penting sebelum dilakukan desain metode/teknologi alternatif untuk esktrasi karotenoid. Pola produk molekuler kimia merupakan salah satu metode yang saat ini menjadi lebih populer untuk mencari pelarut dengan sifat-sifat yang dikehendaki sebelum diujicobakan. Kata kunci :    karotenoid, ekstrasi, pemisahan, teknologi, minyak sawit kasar, limbah industri pengolahan sawit.


2021 ◽  
Vol 9 (2) ◽  
pp. 416
Author(s):  
Charles Dumolin ◽  
Charlotte Peeters ◽  
Evelien De Canck ◽  
Nico Boon ◽  
Peter Vandamme

Culturomics-based bacterial diversity studies benefit from the implementation of MALDI-TOF MS to remove genomically redundant isolates from isolate collections. We previously introduced SPeDE, a novel tool designed to dereplicate spectral datasets at an infraspecific level into operational isolation units (OIUs) based on unique spectral features. However, biological and technical variation may result in methodology-induced differences in MALDI-TOF mass spectra and hence provoke the detection of genomically redundant OIUs. In the present study, we used three datasets to analyze to which extent hierarchical clustering and network analysis allowed to eliminate redundant OIUs obtained through biological and technical sample variation and to describe the diversity within a set of spectra obtained from 134 unknown soil isolates. Overall, network analysis based on unique spectral features in MALDI-TOF mass spectra enabled a superior selection of genomically diverse OIUs compared to hierarchical clustering analysis and provided a better understanding of the inter-OIU relationships.


2003 ◽  
Vol 83 (4) ◽  
pp. 695-712 ◽  
Author(s):  
Ronaldo F. Hashimoto ◽  
Edward.R. Dougherty ◽  
Marcel Brun ◽  
Zheng-Zheng Zhou ◽  
Michael L. Bittner ◽  
...  

2011 ◽  
Vol 76 (1) ◽  
pp. 88-94 ◽  
Author(s):  
Jamie S. Sanderlin ◽  
Nicole Lazar ◽  
Michael J. Conroy ◽  
Jaxk Reeves

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