Energetic characterization of a bioactive compound: Uridine

2018 ◽  
Vol 124 ◽  
pp. 90-97 ◽  
Author(s):  
Piotr Szterner ◽  
Luísa M.P.F. Amaral ◽  
Victor M.F. Morais ◽  
Maria D.M.C. Ribeiro da Silva ◽  
Manuel A.V. Ribeiro da Silva
Keyword(s):  
Bioactive Compound ◽  
Energetic Characterization

scholarly journals Comparative transcriptome analysis to identify candidate genes involved in 2-methoxy-1,4-naphthoquinone (MNQ) biosynthesis in Impatiens balsamina L.

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Lian Chee Foong ◽  
Jian Yi Chai ◽  
Anthony Siong Hock Ho ◽  
Brandon Pei Hui Yeo ◽  
Yang Mooi Lim ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Early Stage ◽  
Expression Profiles ◽  
Bioactive Compound ◽  
Anticancer Activities ◽  
Quinone Oxidoreductase ◽  
Impatiens Balsamina ◽  
Genes Expression ◽  
Pcr Method

Abstract Impatiens balsamina L. is a tropical ornamental and traditional medicinal herb rich in natural compounds, especially 2-methoxy-1,4-naphthoquinone (MNQ) which is a bioactive compound with tested anticancer activities. Characterization of key genes involved in the shikimate and 1,4-dihydroxy-2-naphthoate (DHNA) pathways responsible for MNQ biosynthesis and their expression profiles in I. balsamina will facilitate adoption of genetic/metabolic engineering or synthetic biology approaches to further increase production for pre-commercialization. In this study, HPLC analysis showed that MNQ was present in significantly higher quantities in the capsule pericarps throughout three developmental stages (early-, mature- and postbreaker stages) whilst its immediate precursor, 2-hydroxy-1,4-naphthoquinone (lawsone) was mainly detected in mature leaves. Transcriptomes of I. balsamina derived from leaf, flower, and three capsule developmental stages were generated, totalling 59.643 Gb of raw reads that were assembled into 94,659 unigenes (595,828 transcripts). A total of 73.96% of unigenes were functionally annotated against seven public databases and 50,786 differentially expressed genes (DEGs) were identified. Expression profiles of 20 selected genes from four major secondary metabolism pathways were studied and validated using qRT-PCR method. Majority of the DHNA pathway genes were found to be significantly upregulated in early stage capsule compared to flower and leaf, suggesting tissue-specific synthesis of MNQ. Correlation analysis identified 11 candidate unigenes related to three enzymes (NADH-quinone oxidoreductase, UDP-glycosyltransferases and S-adenosylmethionine-dependent O-methyltransferase) important in the final steps of MNQ biosynthesis based on genes expression profiles consistent with MNQ content. This study provides the first molecular insight into the dynamics of MNQ biosynthesis and accumulation across different tissues of I. balsamina and serves as a valuable resource to facilitate further manipulation to increase production of MNQ.

Structural and energetic characterization of the emissive RNA alphabet based on the isothiazolo[4,3-d]pyrimidine heterocycle core

2016 ◽  
Vol 18 (27) ◽  
pp. 18045-18053 ◽  
Author(s):  
Mohit Chawla ◽  
Albert Poater ◽  
Romina Oliva ◽  
Luigi Cavallo
Keyword(s):  
Energetic Characterization ◽  
Theoretical Characterization

We present theoretical characterization of fluorescent non-natural nucleobases, tzA, tzG, tzC, and tzU, derived from the isothiazolo[4,3-d]pyrimidine heterocycle.

Structural and energetic characterization of the tautomers 2-benzothiazolinone and 2-hydroxybenzothiazole

2014 ◽  
Vol 1078 ◽  
pp. 197-206 ◽  
Author(s):  
Ana L.R. Silva ◽  
Victor M.F. Morais ◽  
Maria D.M.C. Ribeiro da Silva
Keyword(s):  
Energetic Characterization

scholarly journals Lilium Philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis and Partial Identification of Antimicrobial Metabolites

2021 ◽  
Author(s):  
Shefali Singh ◽  
Vineeta Singh ◽  
Alaa Alhazami ◽  
B.N. Mishra ◽  
Shafiul Haque ◽  
...  
Keyword(s):  
Cell Viability ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Achromobacter Xylosoxidans ◽  
Phytochemical Analysis ◽  
Antimicrobial Potential ◽  
Compound Concentration

The members of the Liliaceae family have been regarded as an excellent source of biologically active compounds. However, the work on antimicrobial potential and characterization of the bioactive fractions of Lilium philadelphicum flower is limited and needs to be explored. The present study reports the antimicrobial potential, anti-inflammatory and anticancer potential of the bioactive fraction extracted from the flower of L. philadelphicum (Red Lily) and characterization of these bioactive compounds. The antimicrobial activity was tested against nine different Gram-positive and Gram-negative bacterial strains. The minimum inhibitory concentration (MIC) values of methanolic extract of L. philadelphicum flower against Acinetobacter bouvetii, Achromobacter xylosoxidans, Bacillus subtilis MTCC 121, Candida albicans MTCC 183, Klebsiella pneumoniae MTCC 3384, and Salmonella typhi MTCC 537 were 25, 50, 12.5, 50, 100 and 50 μg mL-1, respectively. The phytochemical analysis of the extract reveals the presence of phenols, flavonoids, tannins, terpenoids, glycosides, coumarins, and quinones. The cytotoxicity of the partially purified compound against the HepG2 cell line in MTT assay demonstrates up to 90% cell viability with a bioactive compound concentration of 50 μg/ml. However, with the increase in bioactive compound concentration up to 1000 μg/ml results into nearly 80% cell viability, just a minor decline in cell viability suggests the importance of bioactive compounds for suitable therapeutic applications. Spectroscopic studies of the bioactive compound by UV-Visible spectroscopy, FT-Infra Red spectroscopy, Gas Chromatography-Mass Spectrometry (GCMS) as well as its phytochemical analysis suggests the presence of terpenoids moiety, responsible for the antimicrobial property of L. philadelphicum flower.

scholarly journals Monitoring Unfolding of Titin I27 single- and bi-Domain with High-Pressure NMR Spectroscopy

2018 ◽  
Author(s):  
I. Herrada ◽  
P. Barthe ◽  
M. Van Heusden ◽  
K DeGuillen ◽  
L Mammri ◽  
...  
Keyword(s):  
High Pressure ◽  
Force Spectroscopy ◽  
Md Simulations ◽  
Specific Information ◽  
Folding Energy ◽  
3D Scaffold ◽  
Folding Process ◽  
Energetic Characterization ◽  
Volume Kinetics

ABSTRACTA complete description of the pathways and mechanisms of protein folding requires a detailed structural and energetic characterization of the folding energy landscape. Simulations, when corroborated by experimental data yielding global information on the folding process, can provide this level of insight. Molecular Dynamics (MD) has been associated often to force spectroscopy experiments to decipher the unfolding mechanism of titin Ig-like single- or multi-domain, the giant multi-modular protein from sarcomere, yielding information on the sequential events during titin unfolding under stretching. Here, we used high-pressure NMR to monitor the unfolding of titin I27 Ig-like single-domain and tandem. Since this method brings residue-specific information on the folding process, it can provide quasi-atomic details on this process, without the help of MD simulations. Globally, the results of our high-pressure analysis are in agreement with previous results obtained by the association of experimental measurements and MD simulation and/or protein engineering, although the intermediate folding state caused by the early detachment of the AB ß-sheet, often reported in previous works based on MD or force spectroscopy, cannot be detected. On the other hand, the A’G parallel ß-sheet of the ß-sandwich has been confirmed as the Achilles heel of the 3D scaffold: its disruption yields complete unfolding, with very similar characteristics (free energy, unfolding volume, kinetics constant rates) for the two constructs.

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