Characteristics of human bone marrow mscs are sensitive to mononuclear cell separation technique and oxygen tension

Cytotherapy ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. S197
Author(s):  
K.T. Wright ◽  
H. McCarthy ◽  
N. Forsyth ◽  
S. Roberts
Keyword(s):  
Bone Marrow ◽  
Oxygen Tension ◽  
Mononuclear Cell ◽  
Cell Separation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Separation Technique ◽  
Bone Marrow Mscs ◽  
Human Bone Marrow Mscs

Human Bone Marrow MSCs form Cartilage and Mineralized Tissue on Chitosan/Polycaprolactone (CS/PCL) Combined Nanofibrous Scaffolds

2017 ◽  
Vol 17 (3) ◽  
pp. 1771-1778 ◽  
Author(s):  
Sarah Abuelreich ◽  
Muthurangan Manikandan ◽  
Abdullah Aldahmash ◽  
Musaad Alfayez ◽  
Mohammed Fayez Al Rez ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mscs ◽  
Mineralized Tissue ◽  
Nanofibrous Scaffolds ◽  
Human Bone Marrow Mscs

A comprehensive characterization study of human bone marrow mscs with an emphasis on molecular and ultrastructural properties

2011 ◽  
Vol 226 (5) ◽  
pp. 1367-1382 ◽  
Author(s):  
Erdal Karaöz ◽  
Alparslan Okçu ◽  
Gülçin Gacar ◽  
Özlem Sağlam ◽  
Sinan Yürüker ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mscs ◽  
Characterization Study ◽  
Human Bone Marrow Mscs ◽  
Comprehensive Characterization

scholarly journals The osteogenic differentiation of human bone marrow MSCs on HUVEC-derived ECM and β-TCP scaffold

Biomaterials ◽  
2012 ◽  
Vol 33 (29) ◽  
pp. 6998-7007 ◽  
Author(s):  
Yunqing Kang ◽  
Sungwoo Kim ◽  
Julius Bishop ◽  
Ali Khademhosseini ◽  
Yunzhi Yang
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mscs ◽  
Human Bone Marrow Mscs

scholarly journals Exosomal miRNA-21-5p derived from bone marrow mesenchymal stem cells promote osteosarcoma cell proliferation and invasion by targeting PIK3R1

2020 ◽  
Author(s):  
Jin Qi ◽  
Ruihao Zhang ◽  
Zuolong Wu ◽  
Yayi Xia
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteosarcoma Cell ◽  
Bone Marrow Mscs ◽  
Osteosarcoma Cells ◽  
Human Bone Marrow Mscs ◽  
Proliferation And Invasion

Abstract Mesenchymal stem cells (MSCs) are a class of pluripotent cells that can release a large number of exosomes which act as paracrine mediators in tumor associated microenvironment. However, the role of MSC-derived exosomes in pathogenesis and progression of cancer cells especially osteosarcoma has not yet been thoroughly clarified until now. In this study, we established a co-culture model for human bone marrow derived MSCs with osteosarcoma U2OS and MG63 cells, then extraction of exosomes from induced MSCs and study the role of MSC-derived exosomes in the progression of osteosarcoma cell. It was the aim of this study to address potential cell biological effects between MSCs and osteosarcoma cell. we found that MSC-derived exosomes can significantly promote osteosarcoma cells proliferation and invasion. we also found that miR-21-5p were significantly overexpressed in human bone marrow MSCs and MSC-derived exosomes compared with that of human fetal osteoblastic cell line hFOB1.19 by using quantitative realtime polymerase chain reaction (qRT-PCR). Proliferation and invasion of osteosarcoma cells U2OS and MG63 were significantly enhanced by MSC-derived exosomes that were transfected with miR-21-5p. Bioinformatics analysis and dual‐luciferase reporter gene assays validated the targeted relationship between exosomal miR‐21-5p and PIK3R1. Furthermore, we demonstrated that miR-21-5p-abundant exosomes derived human bone marrow MSCs could activate PI3K/Akt/mTOR pathway by suppressing PIK3R1 expression in osteosarcoma cells U2OS and MG63. In conclusion, Our findings provide new insight into the interaction between MSCs and osteosarcoma cells in tumor associated microenvironment. Notably, the use of a miR-21-5p inhibitor has an excellent restraining effect on osteosarcoma proliferation and invasion, which provides therapeutic potential for osteosarcoma in future clinical medicine.

scholarly journals Methylome Analysis of Human Bone Marrow MSCs Reveals Extensive Age- and Culture-Induced Changes at Distal Regulatory Elements

2017 ◽  
Vol 9 (3) ◽  
pp. 999-1015 ◽  
Author(s):  
Kalyan K. Pasumarthy ◽  
Naresh Doni Jayavelu ◽  
Lotta Kilpinen ◽  
Colin Andrus ◽  
Stephanie L. Battle ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Regulatory Elements ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mscs ◽  
Methylome Analysis ◽  
Human Bone Marrow Mscs ◽  
Induced Changes ◽  
Distal Regulatory Elements

scholarly journals Transient Canonical Wnt Stimulation Enriches Human Bone Marrow Mononuclear Cell Isolates for Osteoprogenitors

Stem Cells ◽  
2015 ◽  
Vol 34 (2) ◽  
pp. 418-430 ◽  
Author(s):  
Agnieszka A. Janeczek ◽  
Rahul S. Tare ◽  
Edoardo Scarpa ◽  
Ines Moreno-Jimenez ◽  
Caroline A. Rowland ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mononuclear Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mononuclear Cell ◽  
Canonical Wnt

scholarly journals Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Katsunori Shiraishi ◽  
Naosuke Kamei ◽  
Shunsuke Takeuchi ◽  
Shinobu Yanada ◽  
Hisashi Mera ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mrna Expression ◽  
Cartilage Repair ◽  
Expression Profiles ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mscs ◽  
Cartilage Differentiation ◽  
And Cartilage

Quality evaluation of mesenchymal stem cells (MSCs) based on efficacy would be helpful for their clinical application. In this study, we aimed to find the factors of human bone marrow MSCs relating to cartilage repair. The expression profiles of humoral factors, messenger RNAs (mRNAs), and microRNAs (miRNAs) were analyzed in human bone marrow MSCs from five different donors. We investigated the correlations of these expression profiles with the capacity of the MSCs for proliferation, chondrogenic differentiation, and cartilage repair in vivo. The mRNA expression of MYBL1 was positively correlated with proliferation and cartilage differentiation. By contrast, the mRNA expression of RCAN2 and the protein expression of TIMP-1 and VEGF were negatively correlated with proliferation and cartilage differentiation. However, MSCs from all five donors had the capacity to promote cartilage repair in vivo regardless of their capacity for proliferation and cartilage differentiation. The mRNA expression of HLA-DRB1 was positively correlated with cartilage repair in vivo. Meanwhile, the mRNA expression of TMEM155 and expression of miR-486-3p, miR-148b, miR-93, and miR-320B were negatively correlated with cartilage repair. The expression analysis of these factors might help to predict the ability of bone marrow MSCs to promote cartilage repair.

Sign in / Sign up

Export Citation Format

Share Document