Turmeric (Curcuma longa) attenuates food allergy symptoms by regulating type 1/type 2 helper T cells (Th1/Th2) balance in a mouse model of food allergy

The involvement of counteractiveCD8+T-cell subsets during tumor-specific immune responses was analyzed in a syngeneic murine plasmacytoma model.CD8+Tc cells against the immunogenic IL-10-producing BALB/c plasmacytoma ADJ-PC-5 can be easily induced by immunization of BALB/c mice with X-irradiated ADJ-PC-5 tumor cellsin vivoandin vitro. However, the failure of recipient mice to mount a protective Tc response against the tumor during early stages of a real or simulated tumor growth is not due to immunological ignorance, but depends on the induction of tumor-specific tolerance, involving a population of tumorinducedCD8+T cells that are able to inhibit the generation of tumor-specific Tc cells in a primary ADJ-PC-5-specific MLTC, using IFN-γas a suppressive factor. Whereas most longterm cultivated CD8+ADJ-PC-5-specific Tc lines produce type-1 cytokines on stimulation, at least two of them, which were derived from a primary MLTC, display a type-2 cytokine spectrum. Furthermore, the primaryin vitroTc response against ADJ-PC-5 cells shows characteristics of a Tc2 response. The Tc response is strictly depending on tumor-derived IL-10.CD8+Tc cells that are induced in a primary MLTC do not produce IFN-γ, and the tumor-specific Tc response is enhanced by IL-4 but suppressed by IFN-γor IL-12. In contrast, ADJ-PC- 5-specificCD8+Tc cells from immunized mice are IFN-γproducing Tc1 cells. Since the primaryin vitroTc response against the tumor is suppressed even by the smallest numbers of irradiated ADJ-PC-5-specific Tc1 cells via IFN-γthese Tc1 cells behave similar to the suppressiveCD8+T cells that are induced during early stages of ADJ-PC-5 tumorigenesis.

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Strenuous exercise decreases the percentage of type 1 T cells in the circulation

Journal of Applied Physiology ◽

10.1152/jappl.2001.91.4.1708 ◽

2001 ◽

Vol 91 (4) ◽

pp. 1708-1712 ◽

Cited By ~ 91

Author(s):

Adam Steensberg ◽

Anders Dyhr Toft ◽

Helle Bruunsgaard ◽

Marie Sandmand ◽

Jens Halkjær-Kristensen ◽

...

Keyword(s):

T Cells ◽

Peak Plasma ◽

Maximal Oxygen Consumption ◽

Interferon Γ ◽

Treadmill Running ◽

Strenuous Exercise ◽

Plasma Epinephrine ◽

Lymphocyte Number

Prolonged strenuous exercise is followed by a temporary functional immune impairment. Low numbers of CD4+T helper (Th) and CD8+ T cytotoxic (Tc) cells are found in the circulation. These cells can be divided according to their cytokine profile into type 1 (Th1 and Tc1), which produce interferon-γ and interleukin (IL)-2, and type 2 (Th2 and Tc2) cells, which produce IL-4. The question addressed in the present study was whether exercise affected the relative balance between the circulating levels of these cytokine-producing T cells. Nine male runners performed treadmill running for 2.5 h at 75% of maximal oxygen consumption. The intracellular expression of cytokines was detected following stimulation with ionomycin and phorbol 12-myristate 13-acetate in blood obtained before, during, and after exercise. The percentage of type 1 T cells in the circulation was suppressed at the end of exercise and 2 h after exercise, whereas no changes were found in the percentage of type 2 T cells. Plasma epinephrine correlated negatively with the percentage of circulating CD8+ T cells producing IL-2, whereas peak IL-6 correlated with the percentage of CD8+ IL-4-producing T cells in the circulation. Peak plasma IL-6 correlated with plasma cortisol postrunning. In conclusion, the postexercise decrease in T lymphocyte number is accompanied by a more pronounced decrease in type 1 T cells, which may be linked to high plasma epinephrine. Furthermore, IL-6 may stimulate type 2 T cells, thereby maintaining a relatively unaltered percentage of these cells in the circulation compared with total circulating lymphocyte number.

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Tracking Immunological Responses of Islet Antigen-Specific T Cells in the Nonobese Diabetic (NOD) Mouse Model of Type 1 Diabetes

Methods in Molecular Biology - Type-1 Diabetes ◽

10.1007/7651_2015_293 ◽

2015 ◽

pp. 127-134 ◽

Cited By ~ 1

Author(s):

Terri C. Thayer ◽

F. Susan Wong

Keyword(s):

T Cells ◽

Type 1 Diabetes ◽

Mouse Model ◽

Nod Mouse ◽

Immunological Responses ◽

Nonobese Diabetic ◽

Islet Antigen

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Enhancement by vasoactive intestinal peptide of γ‐interferon production by antigen‐stimulated type 1 helper T cells

The FASEB Journal ◽

10.1096/fasebj.13.2.347 ◽

1999 ◽

Vol 13 (2) ◽

pp. 347-353 ◽

Cited By ~ 17

Author(s):

NABILA JABRANE-FERRAT ◽

DEBRA BLOOM ◽

ADRIAN WU ◽

LI LI ◽

DAVID LO ◽

...

Keyword(s):

T Cells ◽

Vasoactive Intestinal Peptide ◽

Helper T Cells ◽

Interferon Production ◽

Γ Interferon

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Pretreatment of donor mice with granulocyte colony-stimulating factor polarizes donor T lymphocytes toward type-2 cytokine production and reduces severity of experimental graft-versus-host disease

Blood ◽

10.1182/blood.v86.12.4422.bloodjournal86124422 ◽

1995 ◽

Vol 86 (12) ◽

pp. 4422-4429 ◽

Cited By ~ 393

Author(s):

L Pan ◽

J Jr Delmonte ◽

CK Jalonen ◽

JL Ferrara

Keyword(s):

Bone Marrow ◽

T Cells ◽

Acute Gvhd ◽

Cytokine Production ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Graft Versus Host ◽

Stimulating Factor

The incidence and severity of acute graft-versus-host disease (GVHD) after allogeneic transplantation using peripheral blood progenitor cells mobilized by granulocyte colony-stimulating factor (G-CSF) appear to be no worse than those after bone marrow transplantation, despite the presence of large numbers of T cells in the donor infusion. Experimental studies have shown that type-1 T cells (secreting interleukin-2 [IL-2] and interferon-gamma) mediate acute GVHD, whereas type-2 T cells (secreting IL-4 and IL-10) can prevent acute GVHD. We tested the hypothesis that G-CSF modulates T-cell function toward a type-2 response and thus reduces the severity of acute GVHD. B6 mice were injected with G-CSF or diluent for 4 days, and their splenic T cells were stimulated in vitro with alloantigen or mitogen in the absence of G-CSF. T cells from G-CSF-treated mice showed a significant increase in IL-4 production, with a simultaneous decrease in IL-2 and interferon-gamma production in response to both stimuli. We also examined the effect of G-CSF pretreatment of donors in a GVHD model (B6- ->B6D2F1). Survival was significantly improved in recipients of G-CSF- treated donors. Concanavalin-A-induced cytokine production at day 13 after transplantation also showed an increase in IL-4 along with a decrease in IL-2 and IFN-gamma production by splenocytes from recipients of G-CSF-treated bone marrow and T cells. These data show that pretreatment of donors with G-CSF polarizes donor T cells toward the production of type-2 cytokines, which is associated with reduced type-1 cytokine production and reduced severity of acute GVHD.

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