Isolation of a calcium-binding peptide from tilapia scale protein hydrolysate and its calcium bioavailability in rats

2014 ◽  
Vol 6 ◽  
pp. 575-584 ◽  
Author(s):  
Da Chen ◽  
Xinmin Mu ◽  
Hai Huang ◽  
Ruiyan Nie ◽  
Zunying Liu ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Protein Hydrolysate ◽  
Binding Peptide ◽  
Calcium Bioavailability ◽  
Tilapia Scale

Purification and characterisation of a glutamic acid-containing peptide with calcium-binding capacity from whey protein hydrolysate

2015 ◽  
Vol 82 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Shun-Li Huang ◽  
Li-Na Zhao ◽  
Xixi Cai ◽  
Shao-Yun Wang ◽  
Yi-Fan Huang ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Whey Protein ◽  
Calcium Binding ◽  
Protein Hydrolysate ◽  
Binding Capacity ◽  
Reversed Phase ◽  
Exchange Chromatography ◽  
Binding Peptide ◽  
Whey Protein Hydrolysate

The bioavailability of dietary ionised calcium is affected by intestinal basic environment. Calcium-binding peptides can form complexes with calcium to improve its absorption and bioavailability. The aim of this study was focused on isolation and characterisation of a calcium-binding peptide from whey protein hydrolysates. Whey protein was hydrolysed using Flavourzyme and Protamex with substrate to enzyme ratio of 25 : 1 (w/w) at 49 °C for 7 h. The calcium-binding peptide was isolated by DEAE anion-exchange chromatography, Sephadex G-25 gel filtration and reversed phase high-performance liquid chromatography (RP-HPLC). A purified peptide of molecular mass 204 Da with strong calcium binding ability was identified on chromatography/electrospray ionisation (LC/ESI) tandem mass spectrum to be Glu-Gly (EG) after analysis and alignment in database. The calcium binding capacity of EG reached 67·81 μg/mg, and the amount increased by 95% compared with whey protein hydrolysate complex. The UV and infrared spectrometer analysis demonstrated that the principal sites of calcium-binding corresponded to the carboxyl groups and carbonyl groups of glutamic acid. In addition, the amino group and peptide amino are also the related groups in the interaction between EG and calcium ion. Meanwhile, the sequestered calcium percentage experiment has proved that EG-Ca is significantly more stable than CaCl2 in human gastrointestinal tract in vitro. The findings suggest that the purified dipeptide has the potential to be used as ion-binding ingredient in dietary supplements.

Isolation of a Calcium-binding Peptide from Chlorella Protein Hydrolysates

2010 ◽  
Vol 15 (4) ◽  
pp. 282-286 ◽  
Author(s):  
So-Jeong Jeon ◽  
Ji-Hye Lee ◽  
Kyung-Bin Song
Keyword(s):  
Calcium Binding ◽  
Protein Hydrolysates ◽  
Binding Peptide

scholarly journals Functional Calcium Binding Peptides from Pacific Cod (Gadus macrocephalus) Bone: Calcium Bioavailability Enhancing Activity and Anti-Osteoporosis Effects in the Ovariectomy-Induced Osteoporosis Rat Model

Nutrients ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1325 ◽  
Author(s):  
Kai Zhang ◽  
Bafang Li ◽  
Qianru Chen ◽  
Zhaohui Zhang ◽  
Xue Zhao ◽  
...  
Keyword(s):  
Serum Calcium ◽  
Rat Model ◽  
Calcium Binding ◽  
Calcium Absorption ◽  
Intestinal Calcium Absorption ◽  
Pacific Cod ◽  
Pacific Cod Gadus Macrocephalus ◽  
Calcium Bioavailability ◽  
Gadus Macrocephalus ◽  
Binding Peptides

Calcium binding peptides from Pacific cod (Gadus macrocephalus) bone have attracted attention due to their potential effects on bone health. In this study, calcium binding peptides (CBP) were prepared from Pacific cod bone by trypsin and neutral protease. Ultraviolet spectra, circular dichroism (CD), and Fourier transform infrared spectroscopy (FTIR) revealed that carboxyl and amino groups in CBP could bind to Ca2+, and form the peptide-calcium complex (CBP-Ca). Single-pass intestinal perfusion (SPIP) experiments indicated that the intestinal calcium absorption was significantly enhanced (p < 0.01) in CBP-Ca treated Wistar rats. The anti-osteoporosis activity of CBP-Ca was investigated in the ovariectomized (OVX) Wistar rat model. The administration of CBP-Ca significantly (p < 0.01) improved the calcium bioavailability, trabecular bone structure, bone biomechanical properties, bone mineral density, and bone mineralization degree. CBP-Ca notably (p < 0.01) increased serum calcium, however, it remarkably (p < 0.01) reduced the levels of osteocalcin (OCN), bone alkaline phosphatase (BALP), tartrate-resistant acid phosphatase isoform 5b (TRAP5b), and C-telopeptide of type I collagen (CTX-1) in serum. Results suggested that the cod bone derived CBP could bind with calcium, improve the intestinal calcium absorption, calcium bioavailability, and serum calcium, then reduce the bone turnover rate, and thus ameliorate osteoporosis.

scholarly journals Calcium Binding Peptide Motifs from Calmodulin Confer Divalent Ion Selectivity to Elastin-Like Polypeptides

2013 ◽  
Vol 14 (7) ◽  
pp. 2347-2353 ◽  
Author(s):  
Wafa Hassouneh ◽  
Michelle L. Nunalee ◽  
M. Coleman Shelton ◽  
Ashutosh Chilkoti
Keyword(s):  
Calcium Binding ◽  
Ion Selectivity ◽  
Binding Peptide ◽  
Peptide Motifs
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