Protective effects of black currant (Ribes nigrum L.) extract on hydrogen peroxide-induced damage in lung fibroblast MRC-5 cells in relation to the antioxidant activity

AbstractGarlic is a valuable source material for medicines due to its known antitumor, hypolipidaemic, antioxidant, and immunomodulatory effects. This study compares the protective effects of conventionally grown (CG) and in vitro propagated garlic (PG) against hydrogen peroxide-induced cytotoxicity in HepG2 cells and their antioxidant activity. Garlic used in this study was obtained by planting garlic cloves or by planting the transplants of PG directly in the field. At the end of the vegetation period, CG and PG were sampled and extracts prepared for the experiment. Compared to conventionally grown garlic bulbs, PG leafy part yielded significantly higher content of polyphenols, flavonoids and alliin, and also showed equal or higher antioxidant activity, measured by the cell viability test, GSH and ROS level. Moreover, PG can be produced in less time (shorter vegetation period) and with significantly less material (cloves). Significantly higher content of alliin, polyphenols, and flavonoids and significantly higher yield of plant biomass in PG has a great potential to become a new production model with improved garlic properties as a medicine material.

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THE EFFECT OF FERTILIZATION ON ANTIOXIDANT ACTIVITY AND CHEMICAL COMPOSITION OF BLACK CURRANT (RIBES NIGRUM L.) CULTIVAR 'BEN TRON'

Acta Horticulturae ◽

10.17660/actahortic.2008.777.79 ◽

2008 ◽

pp. 517-520

Author(s):

A. Nes ◽

B. Hageberg ◽

N. Opstad ◽

F. Måge

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Ribes Nigrum ◽

Black Currant

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TCQA, A Natural Caffeoylquinic Acid Derivative Attenuates H2O2-Induced Neuronal Apoptosis by Suppressing Phosphorylation of MAPKs Signaling Pathway

Planta Medica ◽

10.1055/a-1683-6361 ◽

2021 ◽

Author(s):

Yue Yang ◽

Yufang Ding ◽

Huan Gao ◽

Xiaowen Jiang ◽

Qingchun Zhao

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Acid Derivative ◽

Neuronal Apoptosis ◽

Quinic Acid ◽

Protective Effects ◽

Caffeoylquinic Acid ◽

Mitogen Activated Protein ◽

Caffeoyl Quinic Acid

Abstract1,3,5-Tri-O-caffeoyl quinic acid is a caffeoylquinic acid derivative isolated from the roots of Arctium lappa L. Our previous studies have revealed that the ethyl acetate extract of the roots of A. lappa L. and the caffeoylquinic acids contained in it possess antioxidant properties, especially 1,3,5-tri-O-caffeoyl quinic acid. The present study aimed to investigate the protective effects of 1,3,5-tri-O-caffeoyl quinic acid against hydrogen peroxide-induced oxidative stress and explore the underlying mechanism. We found that 1,3,5-tri-O-caffeoyl quinic acid prevented the decline of cell viability and excessive release of lactate dehydrogenase induced by hydrogen peroxide. In addition, Hoechst 33 342 staining and Annexin V-PI double staining showed that 1,3,5-tri-O-caffeoyl quinic acid inhibited hydrogen peroxide-induced neuronal cell apoptosis. 1,3,5-Tri-O-caffeoyl quinic acid reduced the excessive production of intracellular reactive oxygen species, decreased the malondialdehyde content, and improved the activity of superoxide dismutase. Furthermore, 1,3,5-tri-O-caffeoyl quinic acid restored the loss of mitochondrial membrane potential in SH-SY5Y cells induced by hydrogen peroxide. 1,3,5-Tri-O-caffeoyl quinic acid downregulated the overexpression of proapoptotic proteins, including Bax, cytochrome c, cleaved caspase-9, and cleaved caspase-3 as well as promoted the expression of the antiapoptotic protein Bcl-2. Moreover, the phosphorylation of mitogen-activated protein kinases induced by hydrogen peroxide was inhibited by 1,3,5-tri-O-caffeoyl quinic acid. Pretreatment with 1,3,5-tri-O-caffeoyl quinic acid also promoted the activation of phosphorylated Akt. Taken together, these findings suggest that 1,3,5-tri-O-caffeoyl quinic acid exerts protective effects against hydrogen peroxide-induced neuronal apoptosis. In addition, inhibition of the mitogen-activated protein kinase signaling pathway and the activation of Akt are implicated in the antioxidant activity of 1,3,5-tri-O-caffeoyl quinic acid, giving new insight in searching for a compound with antioxidant activity for the treatment of oxidative stress-associated neurological diseases.

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Comparison of Phytochemical, Iron Chelating, and Free Radical Scavenging Activity of Fresh Ribes Nigrum (Black Currant) and Nutraceutical C24/7

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2019/v25i430080 ◽

2019 ◽

pp. 1-11

Author(s):

Nweze Chibuzor Carole ◽

Rasaq Nurudeen Olajide ◽

Akawu Grace

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Good Health ◽

Free Radical Scavenging ◽

Free Radical Scavenging Activity ◽

Scavenging Activity ◽

Ribes Nigrum ◽

Black Currant

Background: Black currant (Ribes nigrum L. Grossulariceae) promotes good health. A lot of researches have been carried out on common fruits but little attention is given to indigenous fruits such as Ribes nigrum which promotes good health with its high content of phytochemicals that influences its antioxidant activity in neutralizing free radicals as well as its iron chelating property. Aim of this Study: To compare the phytochemical composition, iron-chelating and the free radical scavenging activity of fresh Ribes nigrum and Nutraceutical C24/7. Place and Duration of Study: Department of Biochemistry and Molecular Biology, Nasarawa State University, Keffi, Nasarawa State, Nigeria between June 2017 and January 2019. Methodology: Fleshy parts of fresh Ribes nigrum fruit was homogenized using an electrical blender and was macerated with70% ethanol (Sigma Aldrich, 99.8%) for 72 hours and then filtered. The filtrate was condensed using a rotary evaporator and the extract was stored at 4°C. Preliminary phytochemical screening was carried out on the extract and the nutraceutical C24/7 caplets using standard procedures and the identified phytochemicals: saponins, tannins, alkaloids and steroids were quantitatively estimated. Iron chelating and total antioxidant activity assay was conducted using the DPPH radical scavenging and spectrophotometric methods respectively on the extract and the nutraceutical C24/7. Values were analyzed using One way analysis of Variance (ANOVA).Values of P<0.05 were considered significant. Results and Conclusion: The findings of this study show that the hydro-ethanol extract of Ribes nigrum contains a large amount of flavonoids, saponins tannins, alkaloids and steroids compounds and exhibits iron chelating and high antioxidant (free radical scavenging) activity compared to nutraceutical-C24/7 caplets.

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