Flow-induced vibration widely exists in fluid power machinery, and there is a great relation between the vibration and engineering construction. In this paper, prototype valve experiment and three-dimensional simulation has been done on the pre-opening sleeve valve. High-frequency minute dynamic pressure sensors are adopted in the valve in experiment. Vibration data with dynamic pressure in different conditions are collected and measured to find out the pulsation characteristics of the valve disc during the experiment. Meanwhile, three-dimensional numerical simulation and analysis on the internal flow field of the valve has carried out, in order to discover the factors of vibration induced by fluid flow. The result shows that the main cause of flow-induced vibration varies in different conditions. At the condition of large opening and large pressure difference between valve inlet and outlet, it is mainly due to vortex-induced vibration. Several ways such as improving damping, avoiding the resonance frequency and changing the type of the valve profile can be taken to weaken the vortex-induced vibration.