Multidrug resistance genes are associated with a 42-kb island TGI1 carrying a complex class 1 integron in Trueperella pyogenes

ABSTRACT Six strains of Salmonella enterica serovar Typhi which were resistant to ampicillin, chloramphenicol, trimethoprim-sulfamethoxazole, streptomycin, tetracycline, and gentamicin were isolated in Korea. This multidrug resistance was transferred by a conjugative plasmid of about 50 kb. The plasmid harbored a class 1 integron, which included six resistance genes, aacA4b, catB8, aadA1, dfrA1, aac(6′)-IIa, and the novel blaP2, in that order. All of the isolates showed the same-size plasmids and the same ribotyping patterns, which suggests a clonal spread of these multidrug-resistant isolates.

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Truncated tni Module Adjacent to the Complex Integron of Salmonella Genomic Island 1 in Salmonella enterica Serovar Virchow

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01015-08 ◽

2008 ◽

Vol 53 (2) ◽

pp. 824-827 ◽

Cited By ~ 8

Author(s):

Benoît Doublet ◽

Chishih Chu ◽

Cheng-Hsun Chiu ◽

Yi-Chin Fan ◽

Axel Cloeckaert

Keyword(s):

Multidrug Resistance ◽

Salmonella Enterica ◽

Genomic Island ◽

Open Reading Frame ◽

Complex Class ◽

Class 1 Integron ◽

Reading Frame ◽

Class 1 ◽

First Time

ABSTRACT Salmonella genomic island 1 was identified for the first time in Salmonella enterica serovar Virchow isolated from humans in Taiwan. The complex class 1 integron conferring multidrug resistance was shown to be inserted within open reading frame (ORF) S023 and contains for the first time a partial transpositional module. The 5-bp target duplication flanking the complex integron suggests that its insertion in ORF S023 was by transposition.

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New Determinants of Aminoglycoside Resistance and Their Association with the Class 1 Integron Gene Cassettes in Trueperella pyogenes

International Journal of Molecular Sciences ◽

10.3390/ijms21124230 ◽

2020 ◽

Vol 21 (12) ◽

pp. 4230

Author(s):

Ewelina Kwiecień ◽

Ilona Stefańska ◽

Dorota Chrobak-Chmiel ◽

Agnieszka Sałamaszyńska-Guz ◽

Magdalena Rzewuska

Keyword(s):

Resistance Genes ◽

Resistance Mechanisms ◽

Aminoglycoside Resistance ◽

Class 1 Integron ◽

Gene Cassettes ◽

Genetic Elements ◽

Trueperella Pyogenes ◽

Broth Microdilution Method ◽

Class 1 ◽

Aminoglycoside Resistance Genes

Trueperella pyogenes is an important opportunistic animal pathogen. Different antimicrobials, including aminoglycosides, are used to treat T. pyogenes infections. The aim of the present study was to evaluate aminoglycoside susceptibility and to detect aminoglycoside resistance determinants in 86 T. pyogenes isolates of different origin. Minimum inhibitory concentration of gentamicin, streptomycin, and kanamycin was determined using a standard broth microdilution method. Genetic elements associated with aminoglycoside resistance were investigated by PCR and DNA sequencing. All studied isolates were susceptible to gentamicin, but 32.6% and 11.6% of them were classified as resistant to streptomycin and kanamycin, respectively. A total of 30 (34.9%) isolates contained class 1 integrons. Class 1 integron gene cassettes carrying aminoglycoside resistance genes, aadA11 and aadA9, were found in seven and two isolates, respectively. Additionally, the aadA9 gene found in six isolates was not associated with mobile genetic elements. Moreover, other, not carried by gene cassettes, aminoglycoside resistance genes, strA-strB and aph(3’)-IIIa, were also detected. Most importantly, this is the first description of all reported genes in T. pyogenes. Nevertheless, the relevance of the resistance phenotype to genotype was not perfectly matched in 14 isolates. Therefore, further investigations are needed to fully explain aminoglycoside resistance mechanisms in T. pyogenes.

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Salmonella Genomic Island 1 Multidrug Resistance Gene Clusters in Salmonella enterica Serovar Agona Isolated in Belgium in 1992 to 2002

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.48.7.2510-2517.2004 ◽

2004 ◽

Vol 48 (7) ◽

pp. 2510-2517 ◽

Cited By ~ 48

Author(s):

Benoît Doublet ◽

Patrick Butaye ◽

Hein Imberechts ◽

David Boyd ◽

Michael R. Mulvey ◽

...

Keyword(s):

Multidrug Resistance ◽

Resistance Gene ◽

Salmonella Enterica ◽

Genomic Island ◽

Gene Clusters ◽

Complex Class ◽

Class 1 Integron ◽

Transposase Gene ◽

New Variant ◽

Class 1

ABSTRACT Salmonella genomic island 1 (SGI1) harbors a multidrug resistance (MDR) gene cluster which is a complex class 1 integron. Variant SGI1 MDR gene clusters conferring different MDR profiles have also been identified in several Salmonella enterica serovars and classified as SGI1-A to -F. A retrospective study was undertaken to characterize MDR regions from serovar Agona strains harboring SGI1 isolated from poultry in Belgium between 1992 and 2002. A total of 171 serovar Agona strains, displaying resistance to at least one antibiotic, were studied for the presence of SGI1. SGI1 was detected in 94 serovar Agona strains. The most prevalent variant was SGI1-A (85%), which harbors within the SGI1 complex class 1 integron a common region (CR1) containing orf513, a putative transposase gene, adjacent to the dfrA10 trimethoprim resistance gene. A new variant SGI1 named SGI1-G was identified in two strains. It consisted of the pse-1 gene cassette, as in SGI1-B, but with additional insertion of the orf513/dfrA10 region structure. Seven strains displaying the typical SGI1 MDR profile (Ap Cm Ff Sm Sp Su Tc) showed genetic variation at the 3′ end of SGI1. These strains harbored the insertion of the CR1 containing orf513 as in SGI1-A, -D, and -G. However, downstream the right end of CR1, they presented different 7.4- to 8.5-kb deletions of the SGI1 3′ end that extended to the chromosomal genes yieE and yieF. These results suggest a possible role of CR1 in deletion formation, as has been reported for some insertion sequences. Pulsed-field gel electrophoresis analysis showed that all the serovar Agona SGI1-carrying strains belonged to a single clone. Thus, SGI1 is largely encountered in serovar Agona strains isolated from poultry in Belgium, the most prevalent variant being SGI1-A. SGI1 MDR region undergoes recombinational events resulting in a diversity of MDR gene clusters.

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Unique Class 1 Integron and Multiple Resistance Genes Co-located on IncHI2 Plasmid Is Associated with the Emerging Multidrug Resistance of Salmonella Indiana Isolated from Chicken in China

Foodborne Pathogens and Disease ◽

10.1089/fpd.2012.1455 ◽

2013 ◽

Vol 10 (7) ◽

pp. 581-588 ◽

Cited By ~ 26

Author(s):

Jing Lai ◽

Yang Wang ◽

Jianzhong Shen ◽

Ruichao Li ◽

Jing Han ◽

...

Keyword(s):

Multidrug Resistance ◽

Resistance Genes ◽

Multiple Resistance ◽

Class 1 Integron ◽

Class 1 ◽

Multiple Resistance Genes

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Tn6450, a Novel Multidrug Resistance Transposon Characterized in a Proteus mirabilis Isolate from Chicken in China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02192-17 ◽

2018 ◽

Vol 62 (4) ◽

pp. e02192-17 ◽

Cited By ~ 7

Author(s):

Yan-Peng Chen ◽

Chang-Wei Lei ◽

Ling-Han Kong ◽

Jin-Xin Zeng ◽

Xiu-Zhong Zhang ◽

...

Keyword(s):

Multidrug Resistance ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Proteus Mirabilis ◽

Mobile Elements ◽

Fluoroquinolone Resistance ◽

Class 1 Integron ◽

Content Type ◽

Antimicrobial Resistance Genes ◽

Class 1

ABSTRACT A novel 65.8-kb multidrug resistance transposon, designated Tn6450, was characterized in a Proteus mirabilis isolate from chicken in China. Tn6450 contains 18 different antimicrobial resistance genes, including cephalosporinase gene blaDHA-1 and fluoroquinolone resistance genes qnrA1 and aac(6′)-Ib-cr. It carries a class 1/2 hybrid integron composed of intI2 and a 3′ conserved segment of the class 1 integron. Tn6450 is derived from Tn7 via acquisition of new mobile elements and resistance genes.

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PGI2 Is a Novel SGI1-Relative Multidrug-Resistant Genomic Island Characterized in Proteus mirabilis

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00019-18 ◽

2018 ◽

Vol 62 (5) ◽

Cited By ~ 10

Author(s):

Chang-Wei Lei ◽

Yan-Peng Chen ◽

Ling-Han Kong ◽

Jin-Xin Zeng ◽

Yong-Xiang Wang ◽

...

Keyword(s):

Escherichia Coli ◽

Multidrug Resistance ◽

Proteus Mirabilis ◽

Genomic Island ◽

Multidrug Resistant ◽

Complex Class ◽

Helper Plasmid ◽

Class 1 Integron ◽

Content Type ◽

Class 1

ABSTRACT A novel 61,578-bp genomic island named Proteus genomic island 2 (PGI2) was characterized in Proteus mirabilis of swine origin in China. The 23.85-kb backbone of PGI2 is related to those of Salmonella genomic island 1 and Acinetobacter genomic island 1. The multidrug resistance (MDR) region of PGI2 is a complex class 1 integron containing 14 different resistance genes. PGI2 was conjugally mobilized in trans to Escherichia coli in the presence of a conjugative IncC helper plasmid.

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Complete Nucleotide Sequence of a Conjugative Plasmid CarryingblaPER-1

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00518-15 ◽

2015 ◽

Vol 59 (6) ◽

pp. 3582-3584 ◽

Cited By ~ 7

Author(s):

Ruichao Li ◽

Marcus Ho Yin Wong ◽

Yuanjie Zhou ◽

Edward Wai-chi Chan ◽

Sheng Chen

Keyword(s):

Multidrug Resistance ◽

Nucleotide Sequence ◽

Complete Nucleotide Sequence ◽

Vibrio Parahaemolyticus ◽

Conjugative Plasmid ◽

Complex Class ◽

Class 1 Integron ◽

Content Type ◽

Transmissible Plasmid ◽

Class 1

ABSTRACTThe nucleotide sequence of a self-transmissible plasmid pVPH1 harboringblaPER-1fromVibrio parahaemolyticuswas determined. pVPH1 was 183,730 bp in size and shared a backbone similar to pAQU1 and pAQU2, differing mainly in an ∼40-kb multidrug resistance (MDR) region. A complex class 1 integron was identified together with ISCR1andblaPER-1(ISCR1-blaPER-1-gst-abct-qacEΔ1-sul1), which was shown to form a circular intermediate playing an important role in the dissemination ofblaPER-1.

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