165 Elucidating the METTL3-m6A epitranscriptome in epidermal development and carcinogenesis

Skin tissues, in particular the epidermis, are severely affected by zinc deficiency. However, the zinc-mediated mechanisms that maintain the cells that form the epidermis have not been established. Here, we report that the zinc transporter ZIP10 is highly expressed in the outer root sheath of hair follicles and plays critical roles in epidermal development. We found that ZIP10 marked epidermal progenitor cell subsets and that ablating Zip10 caused significant epidermal hypoplasia accompanied by down-regulation of the transactivation of p63, a master regulator of epidermal progenitor cell proliferation and differentiation. Both ZIP10 and p63 are significantly increased during epidermal development, in which ZIP10-mediated zinc influx promotes p63 transactivation. Collectively, these results indicate that ZIP10 plays important roles in epidermal development via, at least in part, the ZIP10–zinc–p63 signaling axis, thereby highlighting the physiological significance of zinc regulation in the maintenance of skin epidermis.

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The Membrane-anchored Serine Protease Prostasin (CAP1/PRSS8) Supports Epidermal Development and Postnatal Homeostasis Independent of Its Enzymatic Activity

Journal of Biological Chemistry ◽

10.1074/jbc.m113.541318 ◽

2014 ◽

Vol 289 (21) ◽

pp. 14740-14749 ◽

Cited By ~ 23

Author(s):

Diane E. Peters ◽

Roman Szabo ◽

Stine Friis ◽

Natalia A. Shylo ◽

Katiuchia Uzzun Sales ◽

...

Keyword(s):

Enzymatic Activity ◽

Serine Protease ◽

Epidermal Development

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Genetic analysis of Ras genes in epidermal development and tumorigenesis

Small GTPases ◽

10.4161/sgtp.26905 ◽

2013 ◽

Vol 4 (4) ◽

pp. 236-241 ◽

Cited By ~ 6

Author(s):

Matthias Drosten ◽

Carmen G Lechuga ◽

Mariano Barbacid

Keyword(s):

Genetic Analysis ◽

Ras Genes ◽

Epidermal Development

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The unique and cooperative roles of the Grainy head-like transcription factors in epidermal development reflect unexpected target gene specificity

Developmental Biology ◽

10.1016/j.ydbio.2010.11.011 ◽

2011 ◽

Vol 349 (2) ◽

pp. 512-522 ◽

Cited By ~ 62

Author(s):

Yeliz Boglev ◽

Tomasz Wilanowski ◽

Jacinta Caddy ◽

Vishwas Parekh ◽

Alana Auden ◽

...

Keyword(s):

Transcription Factors ◽

Target Gene ◽

Epidermal Development

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Master regulatory role of p63 in epidermal development and disease

Cellular and Molecular Life Sciences ◽

10.1007/s00018-017-2701-z ◽

2017 ◽

Vol 75 (7) ◽

pp. 1179-1190 ◽

Cited By ~ 47

Author(s):

Eduardo Soares ◽

Huiqing Zhou

Keyword(s):

Regulatory Role ◽

Epidermal Development

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Regulation of proneural gene expression and cell fate during neuroblast segregation in the Drosophila embryo

Development ◽

10.1242/dev.114.4.939 ◽

1992 ◽

Vol 114 (4) ◽

pp. 939-946 ◽

Cited By ~ 33

Author(s):

J.B. Skeath ◽

S.B. Carroll

Keyword(s):

Gene Expression ◽

Cell Fate ◽

Single Cells ◽

Regulatory Protein ◽

Drosophila Embryo ◽

Proneural Gene ◽

Developmental Pathway ◽

Neurogenic Genes ◽

Temporal And Spatial ◽

Epidermal Development

The Drosophila embryonic central nervous system develops from sets of progenitor neuroblasts which segregate from the neuroectoderm during early embryogenesis. Cells within this region can follow either the neural or epidermal developmental pathway, a decision guided by two opposing classes of genes. The proneural genes, including the members of the achaete-scute complex (AS-C), promote neurogenesis, while the neurogenic genes prevent neurogenesis and facilitate epidermal development. To understand the role that proneural gene expression and regulation play in the choice between neurogenesis and epidermogenesis, we examined the temporal and spatial expression pattern of the achaete (ac) regulatory protein in normal and neurogenic mutant embryos. The ac protein is first expressed in a repeating pattern of four ectodermal cell clusters per hemisegment. Even though 5–7 cells initially express ac in each cluster, only one, the neuroblast, continues to express ac. The repression of ac in the remaining cells of the cluster requires zygotic neurogenic gene function. In embryos lacking any one of five genes, the restriction of ac expression to single cells does not occur; instead, all cells of each cluster continue to express ac, enlarge, delaminate and become neuroblasts. It appears that one key function of the neurogenic genes is to silence proneural gene expression within the nonsegregating cells of the initial ectodermal clusters, thereby permitting epidermal development.

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Autonomous and nonautonomous Notch functions for embryonic muscle and epidermis development in Drosophila

Development ◽

10.1242/dev.122.2.617 ◽

1996 ◽

Vol 122 (2) ◽

pp. 617-626 ◽

Cited By ~ 10

Author(s):

R. Baker ◽

G. Schubiger

Keyword(s):

Muscle Development ◽

N Gene ◽

Somatic Muscle ◽

Notch Protein ◽

Proper Number ◽

Cellular Domain ◽

A Cell ◽

Embryonic Muscle ◽

Drosophila Embryos ◽

Epidermal Development

The Notch (N) gene encodes a cell signaling protein that mediates neuronal and epidermal determination in Drosophila embryos. N also regulates several aspects of myogenic development; embryos lacking N function have too many muscle founder cells and fail to properly differentiate somatic muscle. To identify cell-autonomous requirements for Notch function during muscle development, we expressed a Notch minigene in the mesoderm, but not in the ectoderm, of amorphic N-embryos. In these embryos, muscle founder hypertrophy is rescued, indicating that Notch is autonomously required by mesoderm cells to regulate the proper number of muscle founders. However, somatic muscle differentiation is only partially normalized, suggesting that Notch is also required in the ectoderm for proper muscle development. Additionally, mesodermal expression of Notch partially rescues epidermal development in overlying neurogenic ectoderm. This is unexpected, since previous studies suggest that Notch is autonomously required by proneural ectoderm cells for epidermal development. Mesodermal expression of a truncated Notch protein lacking the extracellular domain does not rescue ventral epidermis, suggesting that the extra-cellular domain of Notch can non-autonomously rescue epidermal development across germ layers.

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Genome-wide characterization of NtHD-ZIP IV: different roles in abiotic stress response and glandular Trichome induction

BMC Plant Biology ◽

10.1186/s12870-019-2023-4 ◽

2019 ◽

Vol 19 (1) ◽

Author(s):

Hongying Zhang ◽

Xudong Ma ◽

Wenjiao Li ◽

Dexin Niu ◽

Zhaojun Wang ◽

...

Keyword(s):

Abiotic Stress ◽

Stress Response ◽

Stress Responses ◽

Leucine Zipper ◽

Regulatory Gene ◽

Glandular Trichomes ◽

Glandular Trichome ◽

Tissue Expression ◽

Abiotic Stress Response ◽

Epidermal Development

Abstract Background The plant-specific homeodomain-leucine zipper class IV (HD-ZIP IV) gene family has been involved in the regulation of epidermal development. Results Fifteen genes coding for HD-ZIP IV proteins were identified (NtHD-ZIP-IV-1 to NtHD-ZIP-IV-15) based on the genome of N. tabacum. Four major domains (HD, ZIP, SAD and START) were present in these proteins. Tissue expression pattern analysis indicated that NtHD-ZIP-IV-1, − 2, − 3, − 10, and − 12 may be associated with trichome development; NtHD-ZIP-IV-8 was expressed only in cotyledons; NtHD-ZIP-IV-9 only in the leaf and stem epidermis; NtHD-ZIP-IV-11 only in leaves; and NtHD-ZIP-IV-15 only in the root and stem epidermis. We found that jasmonates may induce the generation of glandular trichomes, and that NtHD-ZIP-IV-1, − 2, − 5, and − 7 were response to MeJA treatment. Dynamic expression under abiotic stress and after application of phytohormones indicated that most NtHD-ZIP IV genes were induced by heat, cold, salt and drought. Furthermore, most of these genes were induced by gibberellic acid, 6-benzylaminopurine, and salicylic acid, but were inhibited by abscisic acid. NtHD-ZIP IV genes were sensitive to heat, but insensitive to osmotic stress. Conclusion NtHD-ZIP IV genes are implicated in a complex regulatory gene network controlling epidermal development and abiotic stress responses. The present study provides evidence to elucidate the gene functions of NtHD-ZIP IVs during epidermal development and stress response.

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