Application of high order derivative spectrophotometry to resolve the spectra overlap between BG and MB for the simultaneous determination of them: Ruthenium nanoparticle loaded activated carbon as adsorbent

Abstract High performance liquid chromatography (HPLC) and second-order derivative spectrophotometry have been used for simultaneous determination of pravastatin (PS) and fenofibrate (FF) in pharmaceutical formulations. HPLC separation was performed on a phenyl HYPERSIL C18 column (125 mm × 4.6 mm i.d., 5 μm particle diameter) in the isocratic mode using a mobile phase acetonitrile/0.1 % diethyl amine (50:50, V/V, pH 4.5) pumped at a flow rate of 1.0 mL min-1. Measurement was made at 240 nm. Both drugs were well resolved on the stationary phase, with retention times of 2.15 and 5.79 min for PS and FF, respectively. Calibration curves were linear (R = 0.999 for PS and 0.996 for FF) in the concentration range of 5-50 and 20-200 µg mL-1 for PS and FF, respectively. Pravastatin and fenofibrate were quantitated in combined preparations also using the second-order derivative response at 237.6 and 295.1 nm for PS and FF, respectively. Calibration curves were linear, with the correlation coefficient R = 0.999 for pravastatin and fenofibrate, in the concentration range of 5-20 and 3-20 µg mL-1 for PS and FF, respectively. Both methods were fully validated and compared, the results confirmed that they were highly suitable for their intended purpose.

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Application of Derivative Spectrophotometry for Simultaneous Determination of Quinapril and Hydrochlorothiazide in the Combination Tablets

Journal of AOAC International ◽

10.1093/jaoac/87.4.847 ◽

2004 ◽

Vol 87 (4) ◽

pp. 847-851 ◽

Cited By ~ 2

Author(s):

Dorota Kowalczuk ◽

Hanna Hopkała

Keyword(s):

Standard Deviation ◽

Simultaneous Determination ◽

Relative Standard Deviation ◽

Spectrophotometric Method ◽

Derivative Spectrophotometry ◽

Order Derivative ◽

Zero Crossing ◽

Relative Standard ◽

Separation Of Components

Abstract A new second-order-derivative spectrophotometric method using zero-crossing technique measures quinapril (QUI) and hydrochlorothiazide (HYD) in 2-component mixtures. The procedure does not require prior separation of components from the sample. QUI was determined at a wavelength of 211.6 nm (zero-crossing wavelength point of HYD). Similarly, HYD was measured at 270.8 nm (zero-crossing wavelength point of QUI). Calibration graphs were constructed over the concentration range of 4.0 to 24.0 μ/mL for QUI and 2.5 to 15.0 μg/mL for HYD. Detection and quantitation limits were 0.85 and 2.5 μg/mL for QUI and 0.12 and 0.4 μg/mL for HYD, respectively. The accuracy (recovery 100.5–102%), precision (relative standard deviation less than 3.5% for QUI and 1.5% for HYD), selectivity, and sensitivity of the elaborated methods were satisfactory. The proposed method was applied successfully for the determination of both drugs in QUI-HYD tablets.

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Simultaneous Determination of the Textile Dyes in Industrial Effluents by First-Order Derivative Spectrophotometry

Analytical Sciences ◽

10.2116/analsci.25.487 ◽

2009 ◽

Vol 25 (4) ◽

pp. 487-492 ◽

Cited By ~ 5

Author(s):

Vitor C. ALMEIDA ◽

Alexandro M. M. VARGAS ◽

Juliana C. GARCIA ◽

Ervim LENZI ◽

Cláudio C. OLIVEIRA ◽

...

Keyword(s):

Simultaneous Determination ◽

Industrial Effluents ◽

Textile Dyes ◽

Derivative Spectrophotometry ◽

Order Derivative ◽

First Order

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Rapid simultaneous determination of ruthenium and osmium in aqueous solutions of their tetroxides by second-order derivative spectrophotometry

Analytica Chimica Acta ◽

10.1016/s0003-2670(97)00251-1 ◽

1997 ◽

Vol 349 (1-3) ◽

pp. 53-57 ◽

Cited By ~ 7

Author(s):

M. Balcerzak ◽

E. Świȩcicka

Keyword(s):

Aqueous Solutions ◽

Simultaneous Determination ◽

Second Order ◽

Derivative Spectrophotometry ◽

Order Derivative

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Simultaneous determination of benazepril hydrochloride and hydrochlorothiazide in tablets by second-order derivative spectrophotometry

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/s0731-7085(99)00134-x ◽

1999 ◽

Vol 21 (2) ◽

pp. 257-265 ◽

Cited By ~ 40

Author(s):

I.E Panderi

Keyword(s):

Simultaneous Determination ◽

Second Order ◽

Derivative Spectrophotometry ◽

Order Derivative

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Simultaneous Determination of Nickel and Copper by H-Point Standard Addition MethodFirst-Order Derivative Spectrophotometry in Plant Samples After Separation and Preconcentration on Modified Natural Clinoptilolite as a New Sorbent

Journal of AOAC International ◽

10.1093/jaoac/91.3.637 ◽

2008 ◽

Vol 91 (3) ◽

pp. 637-645 ◽

Cited By ~ 5

Author(s):

Rasool Roohparvar ◽

Mohammad Ali Taher ◽

Alireza Mohadesi

Keyword(s):

Detection Limit ◽

Simultaneous Determination ◽

Solid Phase ◽

Standard Addition ◽

Derivative Spectrophotometry ◽

Order Derivative ◽

Plant Samples ◽

Determination Of Nickel ◽

Separation And Preconcentration

Abstract For the simultaneous determination of nickel(II) and copper(II) in plant samples, a rapid and accurate method was developed. In this method, solid-phase extraction (SPE) and first-order derivative spectrophotometry (FDS) are combined, and the result is coupled with the H-point standard addition method (HPSAM). Compared with normal spectrophotometry, derivative spectrophotometry offers the advantages of increased selectivity and sensitivity. As there is no need for carrying out any pretreatment of the sample, the spectrophotometry method is easy, but because of a high detection limit, it is not so practical. In order to decrease the detection limit, it is suggested to combine spectrophotometry with a preconcentration method such as SPE. In the present work, after separation and preconcentration of Ni(II) and Cu(II) on modified clinoptilolite zeolite that is loaded with 2-[1-(2-hydroxy-5-sulforphenyl)-3-phenyl-5-formazano]-benzoic acid monosodium salt (zincon) as a selective chromogenic reagent, FDS-HPSAM, which is a simple and selective spectrophotometric method, has been applied for simultaneous determination of these ions.With optimum conditions, the detection limit in original solutions is 0.7 and 0.5 ng/mL, respectively, for nickel and copper. The linear concentration ranges in the proposed method for nickel and copper ions in original solutions are 1.1 to 3.0 103 and 0.9 to 2.0 103 ng/mL, respectively. The recommended procedure is applied to successful determination of Cu(II) and Ni(II) in standard and real samples.

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