scholarly journals Application of Derivative Spectrophotometry for Simultaneous Determination of Quinapril and Hydrochlorothiazide in the Combination Tablets

2004 ◽  
Vol 87 (4) ◽  
pp. 847-851 ◽  
Author(s):  
Dorota Kowalczuk ◽  
Hanna Hopkała
Keyword(s):  
Standard Deviation ◽  
Simultaneous Determination ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Derivative Spectrophotometry ◽  
Order Derivative ◽  
Zero Crossing ◽  
Relative Standard ◽  
Separation Of Components

Abstract A new second-order-derivative spectrophotometric method using zero-crossing technique measures quinapril (QUI) and hydrochlorothiazide (HYD) in 2-component mixtures. The procedure does not require prior separation of components from the sample. QUI was determined at a wavelength of 211.6 nm (zero-crossing wavelength point of HYD). Similarly, HYD was measured at 270.8 nm (zero-crossing wavelength point of QUI). Calibration graphs were constructed over the concentration range of 4.0 to 24.0 μ/mL for QUI and 2.5 to 15.0 μg/mL for HYD. Detection and quantitation limits were 0.85 and 2.5 μg/mL for QUI and 0.12 and 0.4 μg/mL for HYD, respectively. The accuracy (recovery 100.5–102%), precision (relative standard deviation less than 3.5% for QUI and 1.5% for HYD), selectivity, and sensitivity of the elaborated methods were satisfactory. The proposed method was applied successfully for the determination of both drugs in QUI-HYD tablets.

scholarly journals Simultaneous Determination of N-Butylscopolamine and Oxazepam in Pharmaceutical Formulations by First-Order Digital Derivative Spectrophotometry

2005 ◽  
Vol 88 (4) ◽  
pp. 1173-1178 ◽  
Author(s):  
Maria Inés Toral ◽  
Marcelo A Muñoz ◽  
Sandra L Orellana
Keyword(s):  
Standard Deviation ◽  
Simultaneous Determination ◽  
Relative Standard Deviation ◽  
Pharmaceutical Formulations ◽  
Derivative Spectrophotometry ◽  
Simple Method ◽  
Zero Crossing ◽  
First Order ◽  
Relative Standard

Abstract A simple method has been developed for the simultaneous determination of N-butylscopolamine bromide and oxazepam in pharmaceutical formulations using first-order digital derivative spectrophotometry. Acetonitrile was selected as the solvent in which both compounds showed well-defined bands. Both analytes showed good stability in this solvent when solutions of the analytes were exposed to light and temperatures between 20° and 80°C. The simultaneous determination of both drugs was performed by the zero-crossing method at 226.0 and 257.0 nm for N-butylscopolamine and oxazepam, respectively. The linear range of determination was found to be 2.5 × 10−7 to 8.0 × 10−5 mol/L for N-butylscopolamine and 7.1 × 10−8 to 8.0 × 10−5 mol/L for oxazepam. A very good level of repeatability (relative standard deviation) of 0.2% was observed for N-butylscopolamine and oxazepam. The ingredients commonly found in pharmaceutical formulations do not interfere. The proposed method was applied to the determination of these drugs in pharmaceutical formulations (capsules).

scholarly journals Derivative spectrophotometric determination of acetamiprid in the presence of 6-chloronicotinic acid

2012 ◽  
Vol 77 (7) ◽  
pp. 911-917 ◽  
Author(s):  
Valéria Guzsvány ◽  
Sanja Lazic ◽  
Natasa Vidakovic ◽  
Zsigmond Papp
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Detection Limits ◽  
Model Systems ◽  
Order Derivative ◽  
Zero Crossing ◽  
First Order ◽  
Relative Standard

A simple first-order derivative spectrophotometric method was developed for the simultaneous determination of acetamiprid and 6-chloronicotinic acid (6-CNA) at pH 7.0. By using the zero-crossing approach, acetamiprid was determined at 269.0 nm and 6-CNA at 216.0 nm with the detection limits of 7.19x10-7 and 8.25x10-7 mol dm-3, respectively and relative standard deviations not exceeding 1.2% in the case of model systems.

scholarly journals Development and validation of spectrophotometric method for phenylephrine hydrochloride estimation in nasal drops formulations

2008 ◽  
Vol 27 (2) ◽  
pp. 149 ◽  
Author(s):  
Ivana Savić ◽  
Goran Nikolić ◽  
Vladimir Banković
Keyword(s):  
Standard Deviation ◽  
Sodium Hydroxide ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Phenylephrine Hydrochloride ◽  
Relative Standard ◽  
Development And Validation

Simple, accurate and reproducible UV-spectrophotometric method was developed and validated for the estimation of phenylephrine hydrochloride in pharmaceutical nasal drops formulations. Phenylephrine hydrochloride was estimated at 291 nm in 1 mol⋅dm-3 sodium hydroxide (pH 13.5). Beer’s law was obeyed in the concentration range of 10–100 μg⋅cm−3 (r2 = 0.9990) in the sodium hydroxide medium. The apparent molar absorptivity was found to be 1.63×103 dm3⋅mol−1⋅cm−1. The method was tested and validated for various parameters according to the ICH (International Conference on Harmonization) guidelines. The detection and quantitation limits were found to be 0.892 and 2.969 μg⋅cm−3, respectively. The proposed method was successfully applied for the determination of phenylephrine hydrochloride in pharmaceutical nasal drops formulations. The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation < 1 %), while being simple, cheap and less time consuming, and hence can be suitably applied for the estimation of phenylephrine hydrochloride in different dosage forms.

scholarly journals IMPORTANCE OF THE OXIDATION REACTION OF SODIUM METAPERIODATE FOR SPECTROPHOTOMETRIC ASSAY OF RESERPINE

2004 ◽  
Vol 72 (2) ◽  
pp. 121-129
Author(s):  
D. K. Singh ◽  
Archana Sahu
Keyword(s):  
Acetic Acid ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Oxidation Reaction ◽  
Spectrophotometric Assay ◽  
Sodium Metaperiodate ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

A new simple, accurate and reasonably sensitive spectrophotometric method is described for the determination of reserpine based on the oxidation with sodium metaperiodate in the presence of acetic acid and coupling with 3-methyl-2-benzo thiazolinone hydazone hydrochloride (MBTH) to form highly blue coloured product showing maximum absorbance at 630 nm. Beer's law is obeyed in the range 0.2–1.6 µg ml−1. The relative standard deviation was found to be less than 1%. The proposed method can be applied for the estimation of reserpine in tablets with no evidence of interference from excipients.

scholarly journals Spectrophotometric Method for Determination of Paraquat in Food and Biological Samples

1997 ◽  
Vol 80 (2) ◽  
pp. 388-391 ◽  
Author(s):  
Ritu Kesari ◽  
Manish Rai ◽  
Vinay Kumar Gupta
Keyword(s):  
Standard Deviation ◽  
Metal Ions ◽  
Relative Standard Deviation ◽  
Alkaline Medium ◽  
Spectrophotometric Method ◽  
Biological Samples ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

Abstract A sensitive spectrophotometric method was developed for determination of paraquat, a widely used herbicide. Paraquat was reduced with glucose in an alkaline medium, and the blue radical ion obtained was measured at 600 nm. Beer’s law was obeyed at 0.1–1.2 ppm paraquat. The molar absorptivity was 1.26 × 105 L mol-1 cm-1. The standard deviation and relative standard deviation were ± 0.007 and 2.0%, respectively, for 5 μg paraquat/10 mL analyzed over 7 days. The method was free from interference by other commonly used pesticides and metal ions. The method may be used to the determine paraquat in plants, fruits, grains, water, blood, and urine.

scholarly journals Simultaneous Determination of Free Carnitine and Total Choline by Liquid Chromatography/Mass Spectrometry in Infant Formula and Health-Care Products: Single-Laboratory Validation

2008 ◽  
Vol 91 (4) ◽  
pp. 777-785 ◽  
Author(s):  
Pierre Andrieux ◽  
Tamara Kilinc ◽  
Christian Perrin ◽  
Esther Campos-Giménez
Keyword(s):  
Health Care ◽  
Standard Deviation ◽  
Simultaneous Determination ◽  
Relative Standard Deviation ◽  
Infant Formula ◽  
Free Carnitine ◽  
Official Method ◽  
Relative Standard ◽  
Single Laboratory

Abstract A single-laboratory validation study was conducted for a liquid chromatographic/mass spectrometric (LC/MS) method for the simultaneous determination of the free carnitine and total choline in milk-based infant formula and health-care products. The sample preparation used for both carnitine and choline was adapted from AOAC Official Method 999.14, with an acidic and enzymatic hydrolysis of esterified forms of choline. Carnitine and choline were quantified by ion-pair chromatography with single-quadrupole MS detection, using their respective deuterated internal standards. The repeatability relative standard deviation was 2.5 and 2.1, respectively, for carnitine and choline. The intermediate reproducibility relative standard deviation was &lt;4.7 and 2.4, respectively, for carnitine and choline. The ranges of the average product-specific recoveries were 9298 and 94103, respectively, for carnitine and choline. Choline concentration determined in infant formula reference material SRM 1846 was in agreement with the reference value. The proposed method was compared with the enzymatic methods for a range of products; good correlation (r = 0.99) was obtained, although a significant bias was observed for both analytes. The method, with a short chromatographic run time (7 min), is convenient for routine analysis to enhance analytical throughput and is a good alternative to enzymatic assays.

Kinetic Spectrophotometric Method for the Determination of Selenium (IV) by its Catalytic Effect on the Reduction of Arsenazo III with Sodium Hypophosphite in Micellar Media

2012 ◽  
Vol 554-556 ◽  
pp. 926-933
Author(s):  
Zhi Rong Zhou ◽  
Li Zhen Zhang
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Catalytic Effect ◽  
Fixed Time ◽  
Sodium Hypophosphite ◽  
Micellar Media ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A simple and sensitive kinetic spectrophotometric method for the determination of trace selenium (IV) is described, based on its catalytic effect on the reduction arsenazo III (AsA III) with sodium hypophosphite (NaH2PO2) in a solution of 0.02 mol/L sulfuric acid and in the presence of cationic micellar media. The reaction rate is monitored spectrophotometrically by measuring the decrease in absorbance of AsA III at 550 nm with a fixed-time method. The decrease in the absorbance of AsA III is proportional to the concentration of Se(IV) in the range 0.16–1.0 µg/L after a fixed time of 4–10 min from the initiation of the reaction. The limit of detection is 0.049 µg/L Se(IV). The influence of th e factors such as acidity, concentration of reactants, type and concentration of surfactants, reactive time, temperature and co-existing ions on the reaction is discussed. The optimum reaction conditions of reaction are established and some kinetic parameters are determined; the apparent activation energy of catalytic reaction is 59.51 kJ/mol. The relative standard deviation for eleven replicate determination of 0.02, and 0.01µg/25mL Se(IV) was 2.0 % and 2.4 %, respectively. Combined with sulphydryl dextrane gel (SDG) separation and enriching, the method has been successfully applied to the determination of Se (IV) in foodstuff and human hair samples with the relative standard deviation of 2.1 %–5.8 % and the recovery of 97.0 %–103.3 %, the results are in good agreement with those provided by ICP-AES method.

The Determination of Nitrate in Tobacco by UV-Spectrometry / Die Bestimmung von Nitrat in Tabak durch UV-Spektrometrie

1966 ◽  
Vol 3 (7) ◽  
Author(s):  
H. Barkemeyer
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Uv Spectrometry ◽  
Relative Standard

AbstractA new spectrophotometric method, suitable for routine determination of nitrate in tobacco, is described. Quantities as low as 0.05 % of nitrate in tobacco are determinable, the results can be reproduced very well (RSD (relative standard deviation) = 1.43 % for 0.7 % of nitrate in tobacco). 15-20 determinations per day and per person can be performed. The figures obtained by this method are identical with those obtained by other methods. Interferences by other compounds have not been observed. Commercial cigarettes show contents of nitrate in tobacco in the range between 2 % and 0.04 %.

scholarly journals UV Spectrophotometric Method Development and Validation of Fimasartan Drug and Its Tablet Formulation

1970 ◽  
Vol 7 (5) ◽  
pp. 25-29
Author(s):  
Kaushik S Agrawal ◽  
Lokesh R Gandhi ◽  
Nitin S. Bhajipale S Bhajipale3
Keyword(s):  
Standard Deviation ◽  
Quantitative Determination ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Method Development ◽  
Cost Effective ◽  
Tablet Formulation ◽  
Relative Standard ◽  
Bulk Drug

A novel, safe and sensitive method of Spectrophotometric estimation in UV - region has been developed for the assay of Fimasartan in its tablet formulation. The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV spectrophotometric method for the estimation of Fimasartan bulk and pharmaceutical formulation. The method have been developed and validated for the assay of Fimasartan using Methanol as diluent. Absorption maximum (λmax) of the drug was found to be 240nm. The quantitative determination of the drug was carried out at 240nm. The method was shown linear in the mentioned concentrations having correlation coefficient R2 of 0.999. The recovery values for Fimasartan ranged from 98.74% to 99.23%.The Percent Relative Standard Deviation of interday and intraday was 0.85% and 0.75% respectively. All the parameters of the analysis were chosen according to the International Conference on Harmonisation guideline and validated statistically using Relative Standard Deviation and Percent Relative Standard Deviation. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.   KEY WORDS: 

Spectrophotometric Determination of Boron in Dates of Some Cultivars Grown in Saudi Arabia

1993 ◽  
Vol 76 (3) ◽  
pp. 601-603 ◽  
Author(s):  
A A Al-Warthan ◽  
S S Al-Showiman ◽  
S A Al-Tamrah ◽  
A A BaOsman
Keyword(s):  
Saudi Arabia ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

Abstract The formation of a red complex between boron and the quinalizarin reagent was investigated and used as the basis for a simple and sensitive spectrophotometric method for boron in date cultivars. At 620 nm, the absorbance was linear (r= 0.999) over the 0.25-2.5 μg/mL concentration range. The molar absorptivity was found to be 2.23 x 103mol-1cm-1 and the relative standard deviation for 10 replicates (1.0 μg/mL) was 0.97%.

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