FL-CTL assay: Fluorolysometric determination of cell-mediated cytotoxicity using green fluorescent protein and red fluorescent protein expressing target cells

2005 ◽  
Vol 300 (1-2) ◽  
pp. 100-114 ◽  
Author(s):  
Kong Chen ◽  
Liansheng Chen ◽  
Peilin Zhao ◽  
Luis Marrero ◽  
Elizabeth Keoshkerian ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Target Cells ◽  
Red Fluorescent Protein ◽  
Green Fluorescent

Excited-State Structure Determination of the Green Fluorescent Protein Chromophore

2005 ◽  
Vol 127 (32) ◽  
pp. 11214-11215 ◽  
Author(s):  
Anwar Usman ◽  
Omar F. Mohammed ◽  
Erik T. J. Nibbering ◽  
Jian Dong ◽  
Kyril M. Solntsev ◽  
...  
Keyword(s):  
Excited State ◽  
Green Fluorescent Protein ◽  
Structure Determination ◽  
Fluorescent Protein ◽  
State Structure ◽  
Green Fluorescent

scholarly journals GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein

1997 ◽  
Vol 327 (3) ◽  
pp. 637-642 ◽  
Author(s):  
B. Paru OATEY ◽  
David H. J. VAN WEERING ◽  
P. Stephen DOBSON ◽  
W. Gwyn GOULD ◽  
Jeremy M. TAVARÉ
Keyword(s):  
Plasma Membrane ◽  
Green Fluorescent Protein ◽  
Glucose Transporter ◽  
Fluorescent Protein ◽  
Time Lapse ◽  
Target Cells ◽  
Green Fluorescent ◽  
Vesicle Movement ◽  
Vesicular Compartment ◽  
Intracellular Structure

Insulin stimulates glucose uptake into its target cells by a process which involves the translocation of the GLUT4 isoform of glucose transporter from an intracellular vesicular compartment(s) to the plasma membrane. The step(s) at which insulin acts in the vesicle trafficking pathway (e.g. vesicle movement or fusion with the plasma membrane) is not known. We expressed a green-fluorescent protein-GLUT4 (GFP-GLUT4) chimaera in 3T3 L1 adipocytes. The chimaera was expressed in vesicles located throughout the cytoplasm and also close to the plasma membrane. Insulin promoted a substantial translocation of GFP-GLUT4 to the plasma membrane. Time-lapse confocal microscopy demonstrated that the majority of GFP-GLUT4-containing vesicles in the basal state were relatively static, as if tethered (or attached) to an intracellular structure. A proportion (approx. 5%) of the vesicles spontaneously lost their tether, and were observed to move rapidly within the cell. Other vesicles appear to be tethered only on one edge and were observed in a rapid stretching motion. The data support a model in which GLUT4-containing vesicles are tightly tethered to an intracellular structure(s), and indicate that a primary site of insulin action must be to release these vesicles, allowing them to then translocate to and fuse with the plasma membrane.

scholarly journals Illuminating the Formation of Lumens

2007 ◽  
Vol 15 (3) ◽  
pp. 3-5
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Animal Model ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Solid Core ◽  
Red Fluorescent Protein ◽  
Neuronal Process ◽  
Two Photon ◽  
Green Fluorescent

How do lumens form? Two mechanisms that come readily to mind are a wrapping model, similar to the wrapping of the myelin sheath around a neuronal process, and a solid core of cells followed by apoptosis of the central cells. Another obvious mechanism that was suggested over 100 years ago is the fusion of intracellular vacuoles. Whereas several recent studies have supported this latter mechanism, it has not yet been proven. Now, the appropriate animal model (zebrafish), the modern techniques (transgenic chimeras), dyes (green fluorescent protein and monomeric red fluorescent protein) that can be linked to proteins to label vacuoles, and two-photon imaging in real time finally have provided the strongest support yet. In an article by Makoto Kamei, Brian Saunders, Kayla Bayless, Louis Dye, George Davis, and Brant Weinstein the assembly of endothelial tubes from intracellular vacuoles was observed in vitro and in vivo.

Development of a fluorescence-linked immunosorbent assay for detection of avermectins using a fluorescent single-domain antibody

2015 ◽  
Vol 7 (9) ◽  
pp. 3728-3734 ◽  
Author(s):  
Min Chen ◽  
Shuangyang Ding ◽  
Kai Wen ◽  
Sanlei Xie ◽  
Qi Wang ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Immunosorbent Assay ◽  
Fluorescent Protein ◽  
Single Domain ◽  
Schematic Diagram ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Green Fluorescent

A schematic diagram of the FLISA based on a fluobody for the determination of AVMs. Note: FLISA, fluorescence-linked immunosorbent assay; GFP, green fluorescent protein; fluobody, fluorescent single-domain antibody; ABM, abamectin; OVA, ovalbumin; and AVMs, avermectins.

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