A simple filter paper-based method for transporting and storing Enterocytozoon hepatopenaei DNA from infected Litopenaeus vannamei tissues

2020 ◽  
Vol 169 ◽  
pp. 107305
Author(s):  
K. Karthikeyan ◽  
Ravi Saranya ◽  
Raja Bharath ◽  
R. Vidya ◽  
Toshiaki Itami ◽  
...  
Keyword(s):  
Filter Paper ◽  
Litopenaeus Vannamei ◽  
Enterocytozoon Hepatopenaei ◽  
Simple Filter

Investigation of parasites in the digestive tract of white leg shrimp (Litopenaeus vannamei) cultured at coastal farms in the Mekong Delta

2021 ◽  
Vol 13 (Aquaculture) ◽  
pp. 79-85
Author(s):  
Thi Hoang Oanh Dang ◽  
Thi Nhu Thuy Nguyen ◽  
Ngoc Ut Vu
Keyword(s):  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Litopenaeus Vannamei ◽  
Developmental Stages ◽  
Slow Growth ◽  
Mekong Delta ◽  
Pcr Analysis ◽  
Histopathological Analysis ◽  
Enterocytozoon Hepatopenaei

A total of 291 white leg shrimp samples were collected from 70 cultured ponds in Soc Trang, Bac Lieu and Ca Mau provinces in the Mekong Delta and subjected to endoparasitic detection in the digestive tract. Collected shrimps displayed unhealthy behaviors such as stop or less feeding and lethagic swimming. Pathological signs in the gastrointestinal tract include (1) empty midgut and stomach together with pale and atrophy hepatopancreas; (2) empty, little or discontinued food in the midgut; (3) slow growth and variation in sizes; and (4) white feces. The results from fresh and Giemsa stained smears methods revealed that 96.5% of sampled shrimps were infected by gregarine parasite at different developmental stages. A prevalence infection of 24.7% was recorded with Vermiform present in the hepatopancreas by fresh smear and histology. Histopathological analysis noted that 7.9% of collected shrimp samples had Enterocytozoon hepatopenaei (EHP) spores in hepatopancreas and midgut and confirmed by PCR analysis.

A simple filter-paper technique allows detection of mucosal cytokine levelsin vivo in ulcerative colitis

1996 ◽  
Vol 41 (9) ◽  
pp. 1775-1779 ◽  
Author(s):  
Jakob Hendel ◽  
Ole H. Nielsen ◽  
Søren Madsen ◽  
Jørn Brynskov
Keyword(s):  
Ulcerative Colitis ◽  
Filter Paper ◽  
Paper Technique ◽  
Simple Filter

Emergence of Enterocytozoon hepatopenaei (EHP) in farmed Penaeus (Litopenaeus) vannamei in India

Aquaculture ◽  
2016 ◽  
Vol 454 ◽  
pp. 272-280 ◽  
Author(s):  
K.V. Rajendran ◽  
Saloni Shivam ◽  
P. Ezhil Praveena ◽  
J. Joseph Sahaya Rajan ◽  
T. Sathish Kumar ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Enterocytozoon Hepatopenaei

Simple Filter Paper Method for Home Monitoring of Blood Glucose, Lactate, and 3-Hydroxybutyrate

1981 ◽  
Vol 18 (4) ◽  
pp. 243-247 ◽  
Author(s):  
Jacqueline M Burrin ◽  
R Worth ◽  
Susan Law ◽  
K G M M Alberti
Keyword(s):  
Blood Glucose ◽  
Boric Acid ◽  
Filter Paper ◽  
Home Monitoring ◽  
Capillary Blood ◽  
Blood Samples ◽  
Diabetic State ◽  
Control Of Diabetes ◽  
Paper Method ◽  
Simple Filter

Previous methods for assessing the control of diabetes at home have concentrated on the measurement of glucose concentrations in urine and, more recently, blood. These levels reflect only one aspect of deranged metabolism in the disease. A technique is described whereby glucose, lactate, and 3-hydroxybutyrate can be measured in capillary blood samples dried on boric acid impregnated filter paper. The technique is sufficiently sensitive to measure 0·05 mmol/l glucose, 0·02 mmol/l lactate, and 0·004 mmol/l 3-hydroxybutyrate with precisions of 4·0%, 4·5%, and 8·0%, respectively. This method could prove useful in the broader metabolic assessment of the diabetic state.

A filter-paper method for determining the moisture characteristics of soil

1967 ◽  
Vol 7 (25) ◽  
pp. 162 ◽  
Author(s):  
RG Fawcett ◽  
N Collis-George
Keyword(s):  
Filter Paper ◽  
Filter Paper Method ◽  
Wide Range ◽  
Field Experimentation ◽  
Moisture Potential ◽  
Paper Method ◽  
Membrane Methods ◽  
Simple Filter

A simple filter paper method for estimating a wide range of moisture potentials has been tested for fifteen soils ranging in texture from sands to heavy clays. The method has given estimates of moisture potential from -0.1 to -900 bars with an accuracy that should be acceptable for many types of field experimentation. Relationships between 15-bar percentages determined by both the filter paper and pressure membrane methods, and biologically determined permanent wilting percentages for wheat, are discussed.

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