Ultrasound imaging of regenerating rat sciatic nerves in situ

AbstractNanodrops comprising a perfluorocarbon liquid core can be acoustically vaporized into echogenic microbubbles for ultrasound imaging. Packaging the microbubble in its condensed liquid state provides distinct advantages, including in situ activation of the acoustic signal, longer circulation persistence, and the advent of expanded diagnostic and therapeutic applications in pathologies which exhibit compromised vasculature. One obstacle to clinical translation is the inability of the limited surfactant present on the nanodrop to encapsulate the greatly expanded microbubble interface, resulting in ephemeral microbubbles with limited utility. In this study, we examine a biomimetic approach to stabilizing an expanding gas surface by employing the lung surfactant replacement, Beractant. Lung surfactant contains a suite of lipids and surfactant proteins that provides efficient shuttling of material from bilayer folds to the monolayer surface. We therefore hypothesized that Beractant would improve stability of acoustically vaporized microbubbles. To test this hypothesis, we characterized Beractant surface dilation mechanics and revealed a novel biophysical phenomenon of rapid interfacial melting, spreading and re-solidification. We then harnessed this unique spreading capability to increase the stability and echogenicity of microbubbles produced after acoustic droplet vaporization for in vivo ultrasound imaging. Such biomimetic lung surfactant-stabilized nanodrops may be useful for applications in ultrasound imaging and therapy.Graphical Abstract

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Color-Doppler ultrasound imaging of in situ human kidney stones in a hyperbaric chamber

The Journal of the Acoustical Society of America ◽

10.1121/1.4969238 ◽

2016 ◽

Vol 140 (4) ◽

pp. 2983-2984

Author(s):

Julianna C. Simon ◽

Barbrina Dunmire ◽

Bryan W. Cunitz ◽

Oleg A. Sapozhnikov ◽

Jeffrey Thiel ◽

...

Keyword(s):

Doppler Ultrasound ◽

Ultrasound Imaging ◽

Kidney Stones ◽

Color Doppler ◽

Human Kidney ◽

Color Doppler Ultrasound ◽

Hyperbaric Chamber ◽

Doppler Ultrasound Imaging

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Magnetomotive ultrasound imaging of rat lymph nodes in situ: Assessment of imaging parameters

2013 IEEE International Ultrasonics Symposium (IUS) ◽

10.1109/ultsym.2013.0156 ◽

2013 ◽

Author(s):

Maria Evertsson ◽

Magnus Cinthio ◽

Sarah Fredriksson ◽

Pontus Kjellman ◽

Rene in 't Zandt ◽

...

Keyword(s):

Lymph Nodes ◽

Ultrasound Imaging ◽

Imaging Parameters ◽

In Situ Assessment

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Musculoskeletal ultrasound for 3D bone modeling: A preliminary study applied to lumbar vertebra

Journal of Back and Musculoskeletal Rehabilitation ◽

10.3233/bmr-200259 ◽

2021 ◽

pp. 1-14

Author(s):

A. Forbes ◽

V. Cantin ◽

Y. Develle ◽

Y. Dubé ◽

A. Bertrand-Grenier ◽

...

Keyword(s):

Ct Scan ◽

Ultrasound Imaging ◽

Lumbar Vertebrae ◽

3D Ultrasound ◽

In Situ Measurements ◽

Musculoskeletal Ultrasound ◽

Bone Modeling ◽

Data Set ◽

Preliminary Study

BACKGROUND: There is no non-invasive in vivo method to assess intervertebral kinematics. Current kinematics models are based on in vitro bone reconstructions from computed tomography (CT)-scan imaging, fluoroscopy and MRIs, which are either expensive or deleterious for human tissues. Musculoskeletal ultrasound is an accessible, easy to use and cost-effective device that allows high-resolution, real-time imaging of bone structure. OBJECTIVE: The aim of this preliminary study was to compare the concordance of 3D bone modeling of lumbar vertebrae between CT-scan and ultrasound imaging and to study the intra and inter-reliability of distances measured on 3D ultrasound bone models. METHODS: CT-scan, ultrasound, and in situ data of five lumbar vertebrae from the same human specimen were used. All vertebrae were scanned by tomography and a new musculoskeletal ultrasound procedure. Then, 3D bone modeling was created from both CT-scan and ultrasound image data set. Distances between anatomical bones landmarks were measured on the 3D models and compared to in situ measurements. We observed that all distances were included within the limit of agreement between the three methods of measurements (3D CT-scan, 3D MSU and in situ) with a good intra- and inter-reliability of 3D ultrasound measurements of 0.97 and 0.82, respectively. Based on the mean of mean differences between methods, we observed for all distances, ultrasound overestimated distances of 0.44 ± 0.63 mm compared to CT-scan, ultrasound underestimated distances of 0.39 ± 0.48 mm compared to in situ measurements and CT-scan underestimated distances of 0.93 ± 0.55 mm compared to in situ measurements. CONCLUSIONS: Three-dimensional modeling from ultrasound imaging is similar in comparison to 3D bone modeling from CT-scan imaging with a good intra and inter reliability.

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In situ conversion of rose bengal microbubbles into nanoparticles for ultrasound imaging guided sonodynamic therapy with enhanced antitumor efficacy

Biomaterials Science ◽

10.1039/c9bm02046b ◽

2020 ◽

Vol 8 (9) ◽

pp. 2526-2536 ◽

Cited By ~ 3

Author(s):

Rui Hou ◽

Xiaolong Liang ◽

Xiaoda Li ◽

Xu Zhang ◽

Xiaotu Ma ◽

...

Keyword(s):

Ultrasound Imaging ◽

Rose Bengal ◽

Drug Accumulation ◽

Antitumor Efficacy ◽

Sonodynamic Therapy

Sonosensitizer microbubbles enhance drug accumulation and the antitumor efficacy of sonodynamic therapy by ultrasound mediated micro to nano conversion.

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In situ ultrasound imaging of shear shock waves in the porcine brain - 3453 words

Journal of Biomechanics ◽

10.1016/j.jbiomech.2021.110913 ◽

2022 ◽

pp. 110913

Author(s):

Sandhya Chandrasekaran ◽

Francisco Santibanez ◽

Bharat B. Tripathi ◽

Ryan DeRuiter ◽

Ruth Vorder Bruegge ◽

...

Keyword(s):

Shock Waves ◽

Ultrasound Imaging ◽

Porcine Brain

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Missing IUCD strings: an analysis

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20184129 ◽

2018 ◽

Vol 7 (10) ◽

pp. 4061

Author(s):

Upasana Verma ◽

Mamta Gupta ◽

Vandana Saini

Keyword(s):

Health Center ◽

Ultrasound Imaging ◽

Obstetric History

Background: Missing IUCD strings, i.e. IUCD strings that are not visible at the external cervical os, are a commonly encountered problem and needs to be analysed.Methods: IUCD users coming for routine checkup (follow-up / renewal / removal) or with any complaints related to IUCD or gynecological complaints or referred from other health center and found to be having missing threads were included in the study. Complaints by woman, obstetric history, details of IUCD insertion, USG imaging reports were recorded. Removal of IUCD was done if indicated or if the woman desired so, by simple OPD procedure and if required in operation theatre.Results: Out of 324 IUCD users who were examined in OPD, missing IUCD threads were found in 69. About half of them were asymptomatic. AUB was the commonest symptom. 82.6% women had IUCD in situ on USG. Displacement was seen in 10.14%, embedment in 4.35%. Expulsion was seen in 4.35% women. Removal was done in only 29 women. Removal was done by simple OPD procedure in 24 women, as minor OT procedure in 3. Only 1 required hysteroscopic removal. Commonest cause of missing threads was found to be broken, detached or severed strings.Conclusions: As most of these women with missed IUCD strings, IUCD was found to be in situ on ultrasound imaging; hence, these women need counselling regarding continuation of using IUCD. If required, removal can be done as an OPD procedure.

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