Characterization of Odontoblast-like Cell Phenotype and Reparative Dentin Formation In Vivo: A Comprehensive Literature Review

2019 ◽  
Vol 45 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Dimitrios Tziafas
Keyword(s):  
Literature Review ◽  
Cell Phenotype ◽  
Comprehensive Literature Review ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

scholarly journals Lithium Chloride Exerts Differential Effects on Dentinogenesis and Osteogenesis in Primary Pulp Cultures

2021 ◽  
Vol 2 ◽  
Author(s):  
Anushree Vijaykumar ◽  
Mina Mina
Keyword(s):  
Dental Pulp ◽  
Osteoblast Differentiation ◽  
Prolonged Treatment ◽  
Odontoblast Differentiation ◽  
Positive Effects ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

Wnt/β-catenin signaling is known to play essential roles in odontoblast differentiation and reparative dentin formation. Various Wnt activators including LiCl have been increasingly studied for their effectiveness to induce repair of the dentin-pulp complex. LiCl is a simple salt thought to activate Wnt/β-catenin signaling by inhibiting GSK3β. Previous in vitro and in vivo studies showed that LiCl increased odontoblast differentiation and enhanced reparative dentin formation. However, the underlying molecular and cellular mechanisms by which LiCl regulates odontoblast and osteoblast differentiation during reparative dentinogenesis are not well-understood. Our in vitro studies show that exposure of early dental pulp progenitors to LiCl increased the survival and the pool of αSMA+ progenitors, leading to enhanced odontoblast and osteoblast differentiation. The positive effects of LiCl in the differentiation of osteoblasts and odontoblasts from αSMA+ progenitors are mediated by Wnt/β-catenin signaling. Our results also showed that continuous and late exposure of dental pulp cells to LiCl increased the expression of odontoblast markers through Wnt/β-catenin signaling, and the number of odontoblasts expressing DMP1-Cherry and DSPP-Cerulean transgenes. However, unlike the early treatment, both continuous and late treatments decreased the expression of Bsp and the expression of BSP-GFPtpz transgene. These observations suggest that prolonged treatment with LiCl in more mature cells of the dental pulp has an inhibitory effect on osteoblast differentiation. The inhibitory effects of LiCl on osteogenesis and Bsp were not mediated through Wnt/β-catenin signaling. These observations suggest that the effects of LiCl, and GSK3β antagonists on reparative dentinogenesis involve multiple pathways and are not specific to Wnt/β-catenin signaling.

scholarly journals Facilitating Reparative Dentin Formation Using Apigenin Local Delivery in the Exposed Pulp Cavity

2021 ◽  
Vol 12 ◽  
Author(s):  
Yam Prasad Aryal ◽  
Chang-Yeol Yeon ◽  
Tae-Young Kim ◽  
Eui-Seon Lee ◽  
Shijin Sung ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Traumatic Injury ◽  
Local Delivery ◽  
Protease Production ◽  
Mice Model ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

Apigenin, a natural product belonging to the flavone class, affects various cell physiologies, such as cell signaling, inflammation, proliferation, migration, and protease production. In this study, apigenin was applied to mouse molar pulp after mechanically pulpal exposure to examine the detailed function of apigenin in regulating pulpal inflammation and tertiary dentin formation. In vitro cell cultivation using human dental pulp stem cells (hDPSCs) and in vivo mice model experiments were employed to examine the effect of apigenin in the pulp and dentin regeneration. In vitro cultivation of hDPSCs with apigenin treatment upregulated bone morphogenetic protein (BMP)- and osteogenesis-related signaling molecules such as BMP2, BMP4, BMP7, bone sialoprotein (BSP), runt-related transcription factor 2 (RUNX2), and osteocalcin (OCN) after 14 days. After apigenin local delivery in the mice pulpal cavity, histology and cellular physiology, such as the modulation of inflammation and differentiation, were examined using histology and immunostainings. Apigenin-treated specimens showed period-altered immunolocalization patterns of tumor necrosis factor (TNF)-α, myeloperoxidase (MPO), NESTIN, and transforming growth factor (TGF)-β1 at 3 and 5 days. Moreover, the apigenin-treated group showed a facilitated dentin-bridge formation with few irregular tubules after 42 days from pulpal cavity preparation. Micro-CT images confirmed obvious dentin-bridge structures in the apigenin-treated specimens compared with the control. Apigenin facilitated the reparative dentin formation through the modulation of inflammation and the activation of signaling regulations. Therefore, apigenin would be a potential therapeutic agent for regenerating dentin in exposed pulp caused by dental caries and traumatic injury.

In Vivo Effects of Amelogenins on Reparative Dentin Formation

2012 ◽  
pp. 174-190 ◽  
Author(s):  
Yassine Harichane ◽  
Sasha Dimitrova-Nakov ◽  
Anne Poliard ◽  
Arthur Veis ◽  
Pamela DenBesten ◽  
...  
Keyword(s):  
Reparative Dentin ◽  
Dentin Formation ◽  
In Vivo Effects ◽  
Reparative Dentin Formation

scholarly journals Dentin Phosphophoryn-Derived Peptide Promotes Odontoblast Differentiation In Vitro and Dentin Regeneration In Vivo

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 874
Author(s):  
Bayarchimeg Altankhishig ◽  
Mohammad Ali Akbor Polan ◽  
Youjing Qiu ◽  
Md Riasat Hasan ◽  
Takashi Saito
Keyword(s):  
Calcium Hydroxide ◽  
Alizarin Red ◽  
Odontoblast Differentiation ◽  
Reparative Dentin ◽  
Pulp Inflammation ◽  
Dentin Formation ◽  
Reparative Dentin Formation ◽  
Dentin Regeneration

The purpose of the present study was to investigate the effect of a peptide (i.e., SESDNNSSSRGDASYNSDES) derived from dentin phosphophoryn (DPP) with arginine-glycine-aspartic acid (RGD) motifs on odontoblast differentiation in vitro and to compare it with calcium hydroxide—a material used conventionally for vital pulp therapy—in terms of reparative dentin formation and pulp inflammation in vivo. Alkaline phosphatase activity assay and alizarin red S staining were performed to evaluate odontoblast-differentiation in cell culturing experiments. To observe the reparative dentin formation and pulp inflammation animal experiment was performed and examined by histological methods. The difference between the experimental group and the control group was analyzed statistically using a one-way ANOVA test. The results revealed that the DPP-derived RGD-containing peptide triggered odontoblast differentiation and mineralization in vitro. In rats undergoing direct pulp capping, the DPP-derived RGD-containing peptide was found to induce intensively formed reparative dentin with high compactness at week 4. On histological and morphometrical examinations, a smaller degree of pulpitis was observed in the specimens treated with the peptide than in those treated with calcium hydroxide. This study suggests that the DPP-derived RGD-containing peptide is a biocompatible, biodegradable and bioactive material for dentin regeneration.

scholarly journals Clinical, molecular, and epidemiological characterization of the SARS-CoV-2 virus and the Coronavirus Disease 2019 (COVID-19), a comprehensive literature review

2020 ◽  
Vol 98 (1) ◽  
pp. 115094 ◽  
Author(s):  
Esteban Ortiz-Prado ◽  
Katherine Simbaña-Rivera ◽  
Lenin Gómez- Barreno ◽  
Mario Rubio-Neira ◽  
Linda P. Guaman ◽  
...  
Keyword(s):  
Literature Review ◽  
Comprehensive Literature Review ◽  
Epidemiological Characterization

scholarly journals Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent

2019 ◽  
Vol 12 (4) ◽  
pp. 182-186
Author(s):  
Mozammal Hossain ◽  
Mahmood Sajedeen ◽  
Yukio Nakamura
Keyword(s):  
Calcium Hydroxide ◽  
Calcium Silicate ◽  
Normal Function ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Exposure Site ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentine formed in the calcium silicate-treated teeth was significantly higher (p<0.005) than in the calcium hydroxide-treated specimens. In conclusion that the calcium silicate indices pulpal wound healing and reparative formation in the exposed teeth without affecting the normal function of the remaining pulp.

Dentonin, a MEPE Fragment, Initiates Pulp-healing Response to Injury

2007 ◽  
Vol 86 (8) ◽  
pp. 780-785 ◽  
Author(s):  
N. Six ◽  
D. Septier ◽  
C. Chaussain-Miller ◽  
R. Blacher ◽  
P. DenBesten ◽  
...  
Keyword(s):  
Synthetic Peptide ◽  
Matrix Proteins ◽  
Dental Tissues ◽  
Agarose Beads ◽  
Reparative Dentin ◽  
Pulp Cells ◽  
Dentin Sialoprotein ◽  
Dentin Formation ◽  
Rat Molars ◽  
Reparative Dentin Formation

Phosphorylated extracellular matrix proteins, including matrix extracellular phosphoprotein (MEPE), are involved in the formation and mineralization of dental tissues. In this study, we evaluated the potential of Dentonin, a synthetic peptide derived from MEPE, to promote the formation of reparative dentin. Agarose beads, either soaked with Dentonin or unloaded, were implanted into the pulps of rat molars, and examined 8, 15, and 30 days after treatment. At day 8, Dentonin promoted the proliferation of pulp cells, as visualized by PCNA-labeling. RP59-positive osteoblast progenitors were located around the Dentonin-soaked beads. PCNA- and RP59-labeling were decreased at day 15, while osteopontin, weakly labeled at day 8, was increased at 15 days, but dentin sialoprotein was undetectable at any time. At 8 days, precocious reparative dentin formation occurred in pulps containing Dentonin-soaked beads, with formation slowing after 15 days. These results suggest that Dentonin affects primarily the initial cascade of events leading to pulp healing.

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